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Glycoconjugated fluorescent labeling reagents

  • US 5,877,310 A
  • Filed: 04/25/1997
  • Issued: 03/02/1999
  • Est. Priority Date: 04/25/1997
  • Status: Expired due to Fees
First Claim
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1. A method for imparting fluorescent properties to a target material, the method comprising the steps of incubating:

  • (i) a target material having at least one functional group selected from the group consisting of carboxylic acid, alkyl halide, aldehyde, ketone, amino, or sulphydryl or having at least one reactive group that can covalently bond with said at least one functional group, and;

    (ii) an amount of a glycoconjugate fluorescent compound having the formula;

    ##STR3## wherein dye is a fluorophone;

    sugar is a sugar or modified carbohydrate rendering the glycoconjugate soluble in aqueous media; and

    Reactive group is a functional group selected from the group consisting of succinimidyl ester, carboxylic acid, isothiocyanate, haloacetamide, maleimide, alkyl halide, azido, hydrazido, aldehyde, ketone, amino sulphydryl, provided said reactive group can covalently bond with said at least one functional group;

    for a period of time sufficient to permit said at least one functional or reactive group of said fluorescent compound to covalently bond to said at least one reactive or functional group of said target material.

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