Reagent dispenser and related test kit for biological specimens

• US 5,879,635 A
• Filed: 03/31/1997
• Issued: 03/09/1999
• Est. Priority Date: 03/31/1997
• Status: Expired due to Term

• Current Assignee: Hygiena Llc (EW GROUP GmbH)
• Original Assignee: Frederic L. Nason
• Inventors: Nason, Frederic L.
• Primary Examiner(s): Thornton, Krisanne
• Application Number:
  US08/829,248
• Time in Patent Office: 708 Days
• Field of Search: 422/61, 422/58, 422/81, 422/100, 422/102, 422/104, 436/174, 436/180, 206/209
• US Class Current: 422/521
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/0272 Dropper bottles
• CPC Class Codes: B65D 81/3211 coaxially and provided with means facilitating admixture
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A reagent dispenser is provided for dispensing multiple reagents particularly for use in analyzing biological specimens and the like. The dispenser includes a pair of reagent chambers with selected reagents therein, and a dual nib for hermetically sealing the reagent chambers. A portion of the dispenser is deformable to break or otherwise to displace the nib in a manner permitting the two reagents to flow together and mix within one of the reagent chambers. The deformable portion or the dispenser can then be squeezed to express the mixed reagents for delivery to contact a specimen to be analyzed. In a preferred form, the dispenser is cap assembly for removable mounting onto an open-ended tubular housing to cooperatively define a specimen chamber within the housing for receiving the specimen on a swab or the like.

Claims

1. A reagent dispenser for dispensing multiple reagents, comprising:

a cap assembly defining a first chamber accommodating a first reagent, a second chamber accommodating a second reagent, and an outlet port communicating at least one of said first and second chambers to the exterior of said cap assembly; and

closure means in said cap assembly for normally separating said chambers and for normally sealing said outlet port;

said cap assembly being sufficiently deformable to open said closure means to permit flow of the first and second reagents through said outlet port to the exterior of said cap assembly.

2. The reagent dispenser of claim 1 wherein said cap assembly is manually deformable to open said closure means.

3. The reagent dispenser of claim 1 wherein said closure means is mounted within said cap assembly.

4. The reagent dispenser of claim 1 wherein said cap assembly is formed from a resilient plastic material.

5. The reagent dispenser of claim 4 wherein said cap assembly is formed from a resilient plastic material that is at least partially transparent.

6. The reagent dispenser of claim 1 further including a passage connecting said first chamber to said second chamber, said closure means being sufficiently deformable to pen said passage.

7. The reagent dispenser of claim 6 wherein said closure means includes a frangible ring for normally sealing and closing said passage, and an elongated nib projecting from said frangible ring into one of said first and second chambers, said cap assembly being sufficiently deformable to bend said nib relative to said frangible ring to rupture said ring and thereby open said passage.

8. The reagent dispenser of claim 7 wherein said nib projects from said frangible ring into said first chamber.

9. The reagent dispenser of claim 7 wherein said closure means further includes a stem pin formed as an extension of said nib, said stem pin having a tip seated within said outlet port for normally sealing said outlet port, said cap assembly being sufficiently deformable to dislodge said stem pin from said outlet port and thereby open said outlet port.

10. The reagent dispenser of claim 6 wherein said closure means includes a frangible ring for normally sealing and closing said outlet port, an elongated nib projecting from said frangible ring, and a stem pin formed as an extension of said nib and having a tip seated within said passage for normally sealing and closing said passage, said cap assembly being sufficiently deformable to bend said nib relative to said frangible ring to rupture said ring and thereby open said outlet port, and further to dislodge said stem pin from said passage and thereby open said passage.

11. The reagent dispenser of claim 6 wherein said closure means includes a seal land press-fit into said passage for normally sealing and closing said passage, and an elongated nib projecting from said seal land into one of said first and second chambers, said cap assembly being sufficiently deformable to bend said nib relative to said seal land to dislodge said seal land from said passage and thereby open said passage.

12. The reagent dispenser of claim 11 wherein said closure means further includes a stem pin formed as an extension of said nib, said stem pin having a tip seated within said outlet port for normally closing and sealing said outlet port, said cap assembly being sufficiently deformable to dislodge said stem pin from said outlet port and thereby open said outlet port.

13. The reagent dispenser of claim 6 wherein said closure means includes a first seal land press-fit into said passage for normally sealing and closing said passage, and a second seal land press-fit into said outlet port for normally sealing and closing said outlet port, said cap assembly being sufficiently deformable relative to said closure means to dislodge said first and second seal lands respectively from said passage and said outlet port to respectively open said passage and said outlet port.

14. The reagent dispenser of claim 6 wherein said closure means comprises a first frangible member for normally closing said passage, a second frangible member for normally closing said outlet port, and nib means connected to said first and second frangible members, said cap assembly being sufficiently deformable to displace said nib means to rupture said first and second frangible members.

15. The reagent dispenser of claim 1 wherein said cap assembly includes a deformable squeeze bulb having at least a portion of said closure means therein.

16. The dual chemistry dispenser of claim 1 wherein said cap assembly includes a deformable corrugated segment having at least a portion of said closure means therein.

17. The reagent dispenser of claim 1 wherein said closure means includes a hollow nib extending from said first chamber and through said second chamber and said outlet port to the exterior of said cap assembly, said hollow nib defining an inflow path for fluid flow into said first chamber, said cap assembly being sufficiently deformable to vacuum draw a fluid through the inflow path into said first chamber.

18. The reagent dispenser of claim 1 further including a hollow tubular housing having an open rear end, said cap assembly being removably mounted on said open rear end of said housing to cooperate with said housing to define a specimen chamber.

19. The reagent dispenser of claim 18 wherein said housing has a dropper tip at a front end thereof.

20. The reagent dispenser of claim 18 wherein said housing has a porous filter plug disposed therein, said filter plug being impregnated with a third reagent.

21. A reagent test kit, comprising:

a cap assembly defining a first chamber for receiving a first reagent, a second chamber for receiving a second reagent, a passage connecting said first chamber to said second chamber, and an outlet port communicating said second chamber to the exterior of said cap assembly;

closure means for normally closing and sealing said passage to maintain said first and second chambers sealed from each other, and for normally closing and sealing said outlet port; and

a hollow housing having an open rear end and adapted for assembly with said cap assembly for cooperation therewith to define a specimen chamber, said second chamber of said cap assembly being positioned with said outlet port for flow communication with said specimen chamber when said cap assembly and said housing are assembled together and when said outlet port is opened by said closure means;

at least one of said cap assembly and said housing being sufficiently deformable to displace said closure means relative to said cap assembly to open said passage and permit mixing of the first and second reagents, and to open said outlet port for flow of mixed reagents from said second chamber to said specimen chamber.

22. The reagent test kit of claim 21 wherein said closure means is mounted within said cap assembly.

23. The reagent test kit of claim 21 wherein said cap assembly is formed from a resilient plastic material.

24. The reagent test kit of claim 23 wherein said cap assembly is formed from a resilient plastic material that is at least partially transparent.

25. The reagent test kit of claim 21 wherein said closure means includes a frangible ring for normally sealing and closing said passage, and an elongated nib projecting from said frangible ring into one of said first and second chambers, said cap assembly being sufficiently deformable to bend said nib relative to said frangible ring to rupture said ring and thereby open said passage.

26. The reagent test kit of claim 25 wherein said nib projects from said frangible ring into said first chamber.

27. The reagent test kit of claim 25 including a porous plug mounted within said second chamber, said porous plug being adapted to be impregnated with the second reagent.

28. The reagent test kit of claim 25 wherein said cap assembly further includes a seal member having said outlet port formed therein for passage of the mixed reagents from said second chamber to said specimen chamber, said closure means further including a stem pin formed as an extension of said nib, said stem pin having a tip seated for normally closing and sealing said outlet port, said cap assembly being sufficiently deformable to displace said stem pin relative to said outlet port and thereby open said outlet port.

29. The reagent test kit of claim 21 wherein said cap assembly further includes a seal member having said outlet port formed therein for passage of the mixed reagents from said second chamber to said specimen chamber, said closure means comprising includes a frangible ring for normally sealing and closing said outlet port, an elongated nib projecting from said frangible ring, and a stem pin formed as an extension of said nib and having a tip seated within said passage for normally sealing and closing said passage, said cap assembly being sufficiently deformable to bend said nib relative to said frangible ring to rupture said ring and thereby open said outlet port, and further to dislodge said stem pin from said passage and thereby open said passage.

30. The reagent test kit of claim 21 wherein said closure means includes a seal land press-fit into said passage for normally sealing and closing said passage, and an elongated nib projecting from said seal land into one of said first and second chambers, said cap assembly being sufficiently deformable to bend said nib relative to said seal land to dislodge said seal land from said passage and thereby open said passage.

31. The reagent test kit of claim 21 wherein said cap assembly further includes a seal member having said outlet port formed therein for passage of the mixed reagents from said second chamber to said specimen chamber, said closure means comprising a first seal land press-fit into said passage for normally sealing and closing said passage, and a second seal land press-fit into said outlet port for normally sealing and closing said outlet port, said cap assembly being sufficiently deformable relative to said closure means to dislodge said first and second seal lands respectively from said passage and said outlet port to respectively open said passage and said outlet port.

32. The reagent test kit of claim 21 wherein said cap assembly includes a deformable squeeze bulb having at least a portion of said closure means therein.

33. The reagent test kit of claim 21 wherein said cap assembly includes a deformable corrugated segment having at least a portion of said closure means therein.

34. The reagent test kit of claim 21 wherein said housing has a dropper tip at a front end thereof.

35. The reagent test kit of claim 21 wherein said housing has a porous filter plug disposed therein, said filter plug being impregnated with a third reagent.

36. A multichamber dispenser, comprising:

a cap assembly defining a first chamber for receiving a first agent, a second chamber for receiving a second . agent, and a passage interconnecting said first and second chambers; and

closure means for normally closing and sealing said passage to maintain said first and second chambers sealed from each other, said closure means defining a fluid inflow path extending from the exterior of said cap assembly and through said second chamber into said first chamber;

said cap assembly being sufficiently deformable to permit vacuum drawing of the first agent through said fluid inflow path into said first chamber, and cap assembly further being sufficiently deformable to displace said closure means relative to said cap assembly to open said passage and permit mixing of the first agent with the second agent in said second chamber, said cap assembly further includes a seal member having an outlet port formed therein for passage of the mixed first and second agents from said second chamber to the exterior of the cap assembly, said closure means extending through said outlet port for normally closing and sealing said second chamber from the exterior of said cap assembly, said cap assembly being sufficiently deformable to displace said closure means relative to said seal member to open said outlet port.

37. The multichamber dispenser of claim 36 wherein said closure means projects a predetermined distance into said first chamber to permit vacuum drawing of a metered amount of the first agent.

38. The multichamber dispenser of claim 37 further including a metered quantity of the second agent in said second chamber.

39. A reagent dispenser for dispensing multiple reagents, comprising:

a cap assembly defining a first chamber for receiving a first reagent, a second chamber for receiving a second reagent, a passage interconnecting said first and second chambers, and means defining an outlet port leading from one of said first and second chambers to the exterior of the dispenser; and

closure means for normally closing and sealing said passage to maintain said first and second chambers sealed from each other, and for normally closing and sealing said outlet port;

said cap assembly being sufficiently deformable to displace said closure means relative to said cap assembly to open said passage and permit mixing of the first and second reagents, and further to open said outlet port to permit the mixed first and second reagents to be expressed through said outlet port to the exterior of the dispenser.

40. The reagent dispenser of claim 39 wherein said cap assembly is sufficiently deformable to express the first reagent from said first chamber for flow through said passage to contact said second reagent within said second chamber.

41. A handheld dispenser for storing first and second reagents and actuatable by finger pressure to dispense the reagents, said dispenser comprising:

a hollow housing enclosing an internal volume, said housing including a peripheral wall with at least a portion thereof deformable by finger pressure from a normal nondeformed position to a deformed position, said housing further defining an outlet port communicating with said internal volume;

a closure member sealing the outlet port when said deformable housing portion is in the normal position and opening said outlet port when said deformable housing portion is in the deformed position; and

a partition separating said internal volume into first and second chambers when said deformable housing portion is in the normal position and communicating said first and second chambers when said deformable housing portion is in the deformed position.

42. The dispenser of claim 41 wherein said closure member and said partition are connected to each other.

43. The dispenser of claim 41 wherein said closure member and said partition are mounted within said housing.

44. The dispenser of claim 42 wherein said partition is mounted within said housing.

45. The dispenser of claim 41 wherein said housing is formed from a resilient plastic material.

46. The dispenser of claim 45 wherein said housing is at least partially transparent.

47. The dispenser of claim 41 wherein said partition includes a frangible ring for normally separating said internal volume into said first and second chambers, and an elongated nib projecting from said frangible ring into one of said first and second chambers, said housing being sufficiently deformable to bend said nib relative to said frangible ring to rupture said ring and thereby communicate said first and second chambers.

48. The dispenser of claim 47 wherein said closure member comprises a stem pin formed as an extension of said nib, said stem pin including means for closing the outlet port when said deformable housing portion is in the normal position, said housing being sufficiently deformable to displace said stem pin relative to said outlet port to open the outlet port.