Method for treating an aqueous protein solution to kill microorganisms therein without causing coagulation and composition thereof

• US 5,882,718 A
• Filed: 03/06/1997
• Issued: 03/16/1999
• Est. Priority Date: 09/20/1994
• Status: Expired due to Term

• Current Assignee: Danish Crown Amba, Novozymes A/S (Novozymes)
• Original Assignee: Novo Nordisk A/S
• Inventors: Pommer, Klaus, Christensen, Egon
• Primary Examiner(s): HEGGESTAD, HELEN F
• Application Number:
  US08/809,540
• Time in Patent Office: 740 Days
• Field of Search: 426/641, 426/646, 426/634, 426/520, 426/46, 426/44, 426/56
• US Class Current: 426/641
• CPC Class Codes: A23B 4/22 Microorganisms; Enzymes; Antibiotics
• CPC Class Codes: A23J 3/341 of animal proteins
• CPC Class Codes: A23J 3/346 of vegetable proteins
• CPC Class Codes: A23L 13/20 from offal, e.g. rinds, skins, marrow, tripes, feet, ears or snouts glands or bones as ingredients of processed meat A23L13/60
• CPC Class Codes: A23L 13/30 Meat extracts
• CPC Class Codes: A23L 13/424 Addition of non-meat animal protein material, e.g. blood, egg, dairy products, fish; Proteins from microorganisms, yeasts or fungi
• CPC Class Codes: A23L 13/426 Addition of proteins, carbohydrates or fibrous material from vegetable origin other than sugars or sugar alcohols A23L13/422 takes precedence
• CPC Class Codes: A23L 13/72 using additives, e.g. by injection of solutions
• CPC Class Codes: C12Y 304/21 Serine endopeptidases (3.4.21)
• CPC Class Codes: C12Y 304/22 Cysteine endopeptidases (3.4.22)
• CPC Class Codes: C12Y 304/23 Aspartic endopeptidases (3.4.23)
• CPC Class Codes: C12Y 304/24 Metalloendopeptidases (3.4.24)

Abstract

A method for treating an aqueous protein solution so as to kill microorganisms which may be present therein, but without causing coagulation, comprises either (1) mixing the solution with sufficient enzymatically produced protein hydrolysate (H1) to prevent coagulation of the mixture when the mixture is subsequently subjected to a heat treatment to kill microorganisms, and then subjecting the mixture to such a heat treatment, or (2) using one or more enzymes to hydrolyze protein in the aqueous protein solution to an extent sufficient to prevent coagulation of the resulting liquid hydrolysate (H2) when it is subsequently subjected to a heat treatment to kill microorganisms, and then subjecting the liquid hydrolysate (H2) to such a heat treatment. The use of aqueous protein solutions treated in this manner in the production of a foodstuff, such as a meat-based foodstuff, contributes very significantly to reducing any risk of contamination of the foodstuff by harmful microorganisms.

Claims

1. A method for treating an aqueous protein solution for use in the production of a foodstuff so as to kill microorganisms which may be present in said protein solution substantially without causing coagulation of the proteinaceous component(s) of said solution, the method comprising the steps of:

either;

mixing said solution with an amount of an enzymatically produced protein hydrolysate (H1) sufficient to substantially prevent coagulation in the mixture when said mixture is subsequently subjected to a heat treatment so as to kill said microorganisms, and

subjecting said mixture to a heat treatment so as to kill said microorganisms;

or;

subjecting said solution to hydrolysis using one or more enzymes so as to at least partly hydrolyze protein in said solution to an extent sufficient to substantially prevent coagulation of the resulting liquid hydrolysate (H2) when said hydrolysate (H2) is subsequently subjected to a heat treatment to kill said microorganisms, and

subjecting said liquid hydrolysate (H2) to a heat treatment to kill said microorganisms.

2. The method according to claim 1, wherein said microorganisms comprise pathogenic microorganisms.

3. The method according to claim 1, wherein said aqueous protein solution comprises protein of animal and/or vegetable origin.

4. The method according to claim 1, wherein said aqueous protein solution comprises meat juice proteins.

5. The method according to claim 1, wherein said aqueous protein solution comprises soya and/or pea proteins.

6. The method according to claim 1, wherein said hydrolysate (H1) is an animal protein hydrolysate produced by hydrolysis of animal protein using one or more proteases.

7. The method according to claim 6, wherein said one or more proteases are proteases of bacterial, fungal, vegetable or animal origin.

8. The method according to claim 7, wherein said one or more proteases comprise a protease selected from the group consisting of serine endopeptidases (E.C. 3.4.21), cysteine endopeptidases (E.C. 3.4.22), aspartic endopeptidases (E.C. 3.4.23) and metalloendopeptidases (3.4.24).

9. The method according to claim 6, wherein said hydrolysate (H1) is a greaves hydrolysate.

10. The method according to claim 1, wherein said one or more enzymes comprise one or more proteases.

11. The method according to claim 1, wherein said foodstuff is a meat-based foodstuff.

12. The method according to claim 11, wherein said meat-based foodstuff is selected from the group consisting of whole-meat foodstuffs and minced-meat foodstuffs.

13. The method according to claim 11, the method further comprising the step of mixing said aqueous protein solution with whole-meat trimmings and/or insoluble animal protein, whereafter the mixture is subjected to said hydrolysis using one or more proteases so as to at least partly hydrolyze protein in said solution and in said whole-meat trimmings and/or insoluble animal protein to an extent sufficient to substantially prevent coagulation of the resulting liquid hydrolysate (H2) when said hydrolysate (H2) is subsequently subjected to a heat treatment to kill said microorganisms.

14. The method according to claim 13, wherein said insoluble animal protein is added in the form of greaves.

15. The method according to claim 1, further comprising the step of removing solids present in said hydrolysate (H2) before and/or after heat treatment thereof.

16. The method according to claim 1, wherein said heat treatment of said mixture or said hydrolysate (H2) comprises heating said mixture or said hydrolysate (H2) to a temperature of at least about 70°

C.

17. The method according to claim 1, wherein said aqueous protein solution is pork meat juice and said protein hydrolysate (H1) is a pork greaves hydrolysate with a protein content (as defined herein) of about 30%, said mixing taking place in a meat juice:

hydrolysate ratio of at most about 20;

80 by weight.

18. A heat-treated protein solution/hydrolysate (H1) mixture or heat-treated liquid hydrolysate (H2) prepared according to claim 1.

19. A method for producing a meat-based foodstuff in which is incorporated an aqueous solution comprising unhydrolysed and/or hydrolysed soluble protein, and preventing contamination of the meat-based foodstuff by pathogenic microorganisms as a direct result of said incorporation, the method comprising adding to the meat forming the basis of said meat-based foodstuff a heat-treated protein solution/hydrolysate (H1) mixture or heat-treated liquid hydrolysate H2 prepared according to claim 11.

20. The method according to claim 19, wherein said meat-based foodstuff is selected from the group consisting of whole-meat foodstuffs and minced-meat foodstuffs.

21. The method according to claim 19, wherein said meat-based foodstuff is a whole-meat foodstuff selected from the group consisting of:

smoked and/or boiled ham;

bacon;

smoked and/or boiled sirloin of beef;

smoked and/or boiled sirloin of pork;

smoked, cured saddle of pork;

smoked and/or boiled, cured poultry meat; and

rolled-meat sausage.

22. The method according to claim 19, wherein said meat-based foodstuff is a minced-meat foodstuff selected from the group consisting of pork sausage, beef sausage and beef hamburger.

23. The method according to claim 19, wherein said meat-based foodstuff is a whole-meat foodstuff, and wherein said addition of said heat-treated protein solution/hydrolysate (H1) mixture or heat-treated liquid hydrolysate (H2) takes place by needle injection thereof into said meat.

24. The method according to claim 19, wherein said meat-based foodstuff is a whole-meat foodstuff, and wherein said addition of said heat-treated protein solution/hydrolysate (H1) mixture or heat-treated liquid hydrolysate (H2) takes place by tumbling of said meat in said heat-treated protein solution/hydrolysate (H1) mixture or heat-treated liquid hydrolysate (H2).

25. The method according to claim 19, wherein said meat-based foodstuff is a minced-meat foodstuff, and wherein said addition of said heat-treated protein solution/hydrolysate (H1) mixture or heat-treated liquid hydrolysate (H2) comprises mixing thereof with said meat and, optionally, other ingredients of said minced-meat foodstuff, said mixing optionally taking place substantially simultaneously with comminution of said meat.

26. A meat-based foodstuff produced by a method according to claim 19.