Gel matrix with redox purple for testing and characterizing microorganisms
First Claim
1. A method for testing microorganisms comprising the steps of:
- a) providing;
i) a testing means,ii) redox purple,iii) one or more test substrates,iv) one or more gel-initiating agents,v) and a gelling agent,vi) an aqueous suspension comprising microorganisms; and
b) introducing said redox purple, said one or more test substrates, and said one or more gel-initiating agents into said testing means to provide a testing system;
c) introducing microorganisms into said testing system; and
d) detecting the response of said microorganisms to said one or more test substrates.
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Accused Products
Abstract
The present invention is directed to methods and compositions for the characterization of various microorganisms. In particular, the present invention is suited for the characterization of commonly encountered microorganisms (e.g., E. coli, S. aureus, etc.), as well as commercially and industrially important organisms from various and diverse environments. For example, the present invention is particularly suited for the growth and characterization of the actinomycetes and fungi. The methods employ a testing system wherein an aqueous suspension of microorganisms is introduced to one or more test substrates comprising redox purple (8-hydroxy-11-methyldibenz- b,e! 1,4!oxazepin-2-(11H)-one) and a gelling agent. The methods detect the response of the microorganisms to the test substrates. A testing device comprising a plurality of testing wells is well suited for the present invention.
30 Citations
20 Claims
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1. A method for testing microorganisms comprising the steps of:
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a) providing; i) a testing means, ii) redox purple, iii) one or more test substrates, iv) one or more gel-initiating agents, v) and a gelling agent, vi) an aqueous suspension comprising microorganisms; and b) introducing said redox purple, said one or more test substrates, and said one or more gel-initiating agents into said testing means to provide a testing system; c) introducing microorganisms into said testing system; and d) detecting the response of said microorganisms to said one or more test substrates. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for testing microorganisms selected from the group consisting of bacteria and fungi, comprising the steps of:
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a) providing a testing device comprising a plurality of testing wells, wherein said wells contain redox purple, a testing substrate and one or more gel-initiating agents; b) preparing a suspension comprising a pure culture of microorganisms and an aqueous solution comprising a gelling agent, under conditions such that said suspension remains ungelled; c) introducing said suspension into said wells of said testing device under conditions such that said suspension forms a gel matrix within said well, such that said microorganisms are within said gel matrix; and d) detecting the response of said microorganisms to said testing substrate. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A method for testing microorganisms selected from the group consisting of bacteria and fungi, comprising the steps of:
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a) providing a testing device comprising a plurality of testing wells, wherein said wells contain a testing substrate and one or more gel-initiating agents; b) preparing a suspension comprising a pure culture of microorganisms and an aqueous solution comprising a gelling agent and redox purple, under conditions such that said suspension remains ungelled; c) introducing said suspension into said wells of said testing device under conditions such that said suspension forms a gel matrix within said well, such that said microorganisms are within said gel matrix; and d) detecting the response of said microorganisms to said testing substrate. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification