Nucleic acids encoding the hypoxia inducible factor-1

• US 5,882,914 A
• Filed: 06/06/1995
• Issued: 03/16/1999
• Est. Priority Date: 06/06/1995
• Status: Expired due to Term

• Current Assignee: Johns Hopkins University
• Original Assignee: Johns Hopkins University
• Inventors: Semenza, Gregg L.
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): Wai, Thanda
• Application Number:
  US08/480,473
• Time in Patent Office: 1,379 Days
• Field of Search: 435/325, 435/252.3, 435/320.1, 536/23.5
• US Class Current: 435/252.3
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: C07K 14/4702 Regulators; Modulating activity

Abstract

The purified and characterization of hypoxiainducible factor 1 (HIF-1) is described. HIF-1 is composed of subunits HIF-1α and HIF-1β. Purified HIF-1α polypeptide, its amino acid sequence and polynucleotide sequence are provided. A HIF-1α variant that dimerizes to HIF-1β producing a nonfunctional HIF-1 complex is described. Methods for the prevention and treatment of hypoxia-related disorders are provided.

Claims

1. An isolated polynucleotide encoding the human hypoxia-inducible factor-1α

polypeptide of SEQ ID NO;

2.

2. The isolated nucleotide sequence of claim 1 selected from the group consisting of:

(a) SEQ ID NO;

1, wherein the nucleotide T can also be the nucleotide U; and

(b) nucleic acid sequences complementary to SEQ ID NO;

1.

3. The nucleotide of claim 1, wherein the nucleotide is isolated from a mammalian cell.

4. The nucleotide of claim 3, wherein the mammalian cell is a human cell.

5. An expression vector including the nucleotide of claim 1.

6. The vector of claim 5, wherein the vector is a plasmid.

7. The vector of claim 5, wherein the vector is a virus.

8. A in vitro host cell stably transformed with the vector of claim 5.

9. The host cell of claim 8, wherein the cell is prokaryotic.

10. The host cell of claim 8, wherein the cell is eukaryotic.

11. An isolated nucleotide sequence encoding the human variant HIF-1α

polypeptide having the sequence of SEQ ID NO;

4.