Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
First Claim
Patent Images
1. A copolymer of acrylylglycinamide and acrylamide, wherein the weight percent ratio of acrylylglycinamide to acrylamide of said copolymer is in the range from about 15:
- 85 to about 99;
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Abstract
Thermoreversible hydrogels comprising non-ionic, uncrosslinked copolymers, and methods of their use in electrophoresis, are provided. The subject copolymers comprise polyacrylamide backbones, where a portion of the acrylamide monomeric units comprise hydrogen bonding groups as N-substituents. Combination of the subject copolymers with an aqueous phase provides thermoreversible hydrogels which find use as separation media in electrophoretic applications.
190 Citations
14 Claims
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1. A copolymer of acrylylglycinamide and acrylamide, wherein the weight percent ratio of acrylylglycinamide to acrylamide of said copolymer is in the range from about 15:
- 85 to about 99;
1.
- 85 to about 99;
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2. A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
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a non-ionic, uncrosslinked copolymer comprising first and second monomeric units in a weight percent ratio of from about 15;
85 to about 99;
1, wherein said first monomeric unit is acrylylglycinamide;and said second monomeric unit is an acrylamide. a continuous liquid phase acrylylglycinamide; and
said second monomeric unit is an acrylamide.
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3. A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
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a polyacrylamide backbone, wherein a portion of the acrylamide monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding, wherein said hydrogen bonding N-substituent groups comprise a carbamyl group; an additional, non-proteinaceous uncrosslinked polymer; and an aqueous phase. - View Dependent Claims (4)
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5. A non-ionic, uncrosslinked copolymer capable of forming a thermoreversible hydrogel when combined with an aqueous phase, said copolymer comprising a polyacrylamide backbone wherein said copolymer comprises first and second monomeric units in a ratio between about 15:
- 85 and 99;
1 by weight, and wherein said first monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding, said N-substituent hydrogen bonding groups comprising a heterocyclic nitrogen base, and said second monomeric unit is an acrylamide. - View Dependent Claims (6)
- 85 and 99;
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7. A non-ionic, uncrosslinked copolymer capable of forming a thermoreversible hydrogel when combined with an aqueous phase, said copolymer comprising first and second monomeric units in a weight percent ratio of from about 15:
- 85 to about 99;
1, whereinsaid first monomeric unit is of the formula;
##STR4## wherein;
X is H or CH3,Y is a bond or a linking group of from 1 to 6 carbon atoms; and Z is a group comprising a hydrogen bonding moiety, wherein said hydrogen bonding moiety comprises a heterocyclic nitrogen base; and said second monomeric unit is an acrylamide. - View Dependent Claims (8)
- 85 to about 99;
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9. A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
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a non-ionic, uncrosslinked copolymer comprising a polyacrylamide backbone, wherein about 15 to 99 weight percent of the acrylamide monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding, wherein said N-substituent groups comprise a heterocyclic nitrogen base; and a continuous fluid phase. - View Dependent Claims (10, 11, 12)
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13. A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
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a non-ionic, uncrosslinked copolymer comprising first and second monomeric units in a weight percent ratio of from about 15;
85 to about 99;
1, wherein said first monomeric unit is of the formula;
##STR5## wherein;
X is H or CH3,Y is a bond or a linking group of from 1 to 6 carbon atoms; and Z is a group comprising a hydrogen bonding moiety, wherein said hydrogen bonding moiety comprises a heterocyclic nitrogen base; and said second monomeric unit is an acrylamide; and a continuous liquid phase. - View Dependent Claims (14)
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Specification