CD9 antigen peptides and antibodies thereto

• US 5,883,223 A
• Filed: 05/30/1995
• Issued: 03/16/1999
• Est. Priority Date: 11/23/1988
• Status: Expired due to Term

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Gary S. Gray
• Inventors: Gray, Gary S.
• Primary Examiner(s): Adams, D.
• Assistant Examiner(s): Gambel, Phillip
• Application Number:
  US08/453,925
• Time in Patent Office: 1,386 Days
• Field of Search: 530/388.75, 530/388.22, 530/350, 530/330, 530/324, 530/395, 530/387.1, 530/300, 530/388.2, 435/240.27, 435/325, 435/332, 435/343.2
• US Class Current: 530/328
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 2039/5158 Antigen-pulsed cells, e.g. T-cells
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 14/70521 CD28, CD152
• CPC Class Codes: C07K 16/2806 against CD2
• CPC Class Codes: C07K 16/2809 against the T-cell receptor (TcR)-CD3 complex
• CPC Class Codes: C07K 16/2812 against CD4
• CPC Class Codes: C07K 16/2815 against CD8
• CPC Class Codes: C07K 16/2818 against CD28 or CD152
• CPC Class Codes: C07K 16/2833 against MHC-molecules, e.g. HLA-molecules
• CPC Class Codes: C07K 16/2866 against receptors for cytokines, lymphokines, interferons
• CPC Class Codes: C07K 16/289 against CD45
• CPC Class Codes: C07K 16/2896 against molecules with a "CD"-designation, not provided for elsewhere
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: C07K 2317/54 F(ab')2
• CPC Class Codes: C07K 2317/74 Inducing cell proliferation
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
• CPC Class Codes: C12N 2501/51 B7 molecules, e.g. CD80, CD86, CD28 (ligand), CD152 (ligand)
• CPC Class Codes: C12N 2501/515 CD3, T-cell receptor complex
• CPC Class Codes: C12N 2501/599 : with CD designations not provided for elsewhere

Abstract

Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

Claims

1. An isolated peptide having an amino acid sequence (Xaa₁)_n -Gly-Xaa₂ -Trp-Leu-Xaa₃ -Xaa₄ -Asp(Glu)-(Xaa₅)_n (SEQ ID NO:

5), wherein Xaa₄ may or may not be present, Xaa₁, Xaa₂, Xaa₃, Xaa₄ and Xaa₅ are any amino acid residue and n=0-20.

2. The isolated peptide of claim 1, wherein Xaa₂ is Cys, Ile or leu, Xaa₃ is Leu or Arg and Xaa₄, if present, is Arg, Pro or Phe.

4. The monoclonal antibody of claim 2, wherein Xaa₂ is Cys, Ile or Leu, Xaa₃ is Leu or Arg and Xaa4, if present, is Arg, Pro or Phe.

5. A hybridoma designated by ATCC Accession No. HB11374, which produces an antibody of claim 2.

6. A monoclonal antibody produced by the hybridoma of claim 5.

3. A monoclonal antibody which specifically binds to a CD9 antigen present on activated T cells, thereby stimulating or enhancing T cell proliferation, wherein the monoclonal antibody specifically binds a peptide having an amino acid sequence (Xaa₁)_n -Gly-Xaa₂ -Trp-Leu-Xaa₃ -Xaa₄ -Asp(Glu)-(Xaa₅)_n (SEQ ID NO:

5), wherein Xaa₄ may or may not be present, Xaa₁, Xaa₂, Xaa₃, Xaa₄ and Xaa₅ are any amino acid residue and n=0-20.