Apparatus and methods for transmitting electrical signals indicative of optical interactions between a light beam and a flowing suspension of particles

• US 5,883,378 A
• Filed: 07/30/1996
• Issued: 03/16/1999
• Est. Priority Date: 07/30/1996
• Status: Expired due to Fees

• Current Assignee: Bayer Corporation (Bayer AG)
• Original Assignee: Bayer Corporation (Bayer AG)
• Inventors: Cantatore, Luigi, Irish, Joseph, Roach, deceased, Paul J., Chaparian, Leon
• Primary Examiner(s): LE, QUE TAN
• Application Number:
  US08/692,935
• Time in Patent Office: 959 Days
• Field of Search: 250/214 A, 250/214 LA, 250/573, 250/222.2, 128/661.08, 330/59, 330/110, 356/244, 356/246, 356/39-42
• US Class Current: 250/214.00A
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 35/00871 Communications between instruments or with remote terminals
• CPC Class Codes: H01S 5/042 Electrical excitation ; Circuits therefor monolithically integrated laser drive components H01S5/0261
• CPC Class Codes: H01S 5/06825 Protecting the laser, e.g. during switch-on/off, detection of malfunctioning or degradation
• CPC Class Codes: H01S 5/0683 by monitoring the optical output parameters

Abstract

A system and methods for transmitting analog signals, particularly analog signals derived from an optical signal intensity, in a noisy environment over a length of cable. The optical signal is sensed by a photodetector, which is bootstrapped and backbiased to minimize the capacitive effect of the photodetector, and converted to a voltage by a transconductance stage. The voltage signal is then driven over a length of cable by a buffer amplifier having a low impedance output substantially matched to the characteristic impedance of the cable (e.g., twin lead wires having a Z₀ =120), to a second circuit board. The cable length may be, e.g, less than a meter. The second circuit board contains a high gain amplifier stage and circuits for processing the high gain amplifier signal to derive, e.g, a peak pulse amplitude corresponding to the peak intensity of the optical signal sensed at the first circuit board. The processing circuit also may include circuits to discriminate valid pulses from invalid pulses so that only valid pulse data is retained. The processing circuitry also may include automatic gain control, attenuation and DC signal restoration, and a test pulse generator for injecting calibrated test pulses into the first circuit board (or the second circuit board) to test the quality of or to calibrate the signal processing function and noise immunity.

Claims

1. Apparatus for detecting an optical signal representing optical interactions between a light beam and a flowing stream of particles and transmitting a low gain electronic output representative of the optical interactions, comprising:

a first circuit board including a high gain amplifier having an input and an output;

a cable having a characteristic impedance, a first end and a second end, the first end being coupled to the high gain amplifier input; and

a second circuit board including;

a first amplifier having a current output;

a photodetector connected to said first amplifier current output, the photodetector having an optical input and an electrical signal output responsive to an optical signal intensity at the optical input;

a second amplifier coupled to said photodetector electrical signal output having a first amplified voltage output;

an RC filter having as an input the first amplified voltage output and having a filtered voltage output corresponding to the filtered first amplified voltage output; and

a third amplifier having as an input said filtered voltage output, and a second amplified output coupled to the second end of the cable, the second amplified output having a low impedance output to match substantially the characteristic impedance of the cable.

2. The apparatus of claim 1 wherein the photodetector further comprises a pin current photodiode and the first amplifier further comprises a current driven bootstrap amplifier having a unity gain, wherein the second amplifier has a predetermined transconductance to relate the photodetector current to a voltage, and the third amplifier is a buffer driver amplifier having a gain sufficient to drive the filtered signals over the cable.

3. The apparatus of claim 2 wherein the photodiode further comprises an anode and a cathode, wherein the anode is coupled to the current output of the first amplifier and a positive reference voltage, and the cathode is connected to the second amplifier, wherein the second amplified output corresponds to a dark field optical input signal.

4. The apparatus of claim 2 wherein the photodiode further comprises an anode and a cathode wherein the cathode coupled to the first amplifier current output and a negative reference voltage, and the anode is connected to the second amplifier wherein the second amplifier output corresponds to a bright field optical input signal.

5. The apparatus of claim 1 wherein the first circuit board further comprises an automatic gain control circuit coupled to said high gain amplifier, having a gain controlled output, an attenuator circuit, and a DC voltage restoration circuit operable to restore a DC offset of the gain controlled output.

6. The apparatus of claim 5 wherein the DC voltage restoration circuit has a predetermined gain.

7. The apparatus of claim 1 wherein the first circuit board further comprises an analog switch interposed between the cable and the high gain amplifier input, and a test generator having a test signal output, wherein the analog switch has a first input coupled to the test generator and a second input coupled to the high gain amplifier input, a first state for passing the second amplifier output to the high gain amplifier input, and a second state for passing the test signal output to the high gain amplifier input.

8. The apparatus of claim 1 wherein the cable has a length of less than one meter.

9. The apparatus of claim 1 wherein the high gain amplifier further comprises a first gain stage and a second gain stage, and the first circuit board and second circuit board further comprise a total gain that is distributed over the first gain stage, the second gain stage, the third amplifier, and a transconductance stage comprising the first amplifier and the second amplifier.

10. The apparatus of claim 2 wherein each of amplifiers is an operational amplifier configured in an inverting amplification mode.

11. A method of transmitting an electrical signal representative of an interaction between a light beam and a flowing stream of particles comprising:

(a) sensing an optical signal having a variable intensity corresponding to the interaction of particles passing through the light beam with a photodetector having a capacitive effect and a sensed optical signal output;

(b) minimizing the capacitive effect of the photodetector by bootstrapping and backbiasing the photodectector;

(c) converting the sensed optical signal from the photodetector to an analog electrical signal;

(d) low pass filtering the analog signal;

(e) driving the filtered analog signal with a buffer amplifier having a low impedance output over a length of cable;

(f) amplifying the low gain signal transmitted over the cable, thereby to provide a high gain signal; and

(g) processing the high gain signal and determining therefrom a peak amplitude indicative of a peak intensity of the optical signal.

12. The method of claim 11 wherein step (f) further comprises:

iii) identifying a valid pulse in response to the high gain amplified signal presenting a pulse having a pulse height within a preselected range of heights and a pulse width within a preselected range of pulse widths; and

iv) storing the determined peak amplitude for a valid pulse identified in step i).

13. The method of claim 12 further comprising injecting a test signal in place of the filtered signal driven over the cable and determining a quality of the processed analog signal identified in step (f) in response to the test signal.

14. The method of claim 11 wherein step (d) further comprises transmitting the signal over a cable having a length of at less than one meter.

15. The method of claim 11 wherein step (d) further comprises using two inverting stages of amplification.

16. The method of claim 15 wherein step (f) further comprises providing automatic gain control of the high gain amplified signal, attenuating the AGC signal for calibration, amplifying the attenuated signal, and DC restoring the amplified high gain signal prior to determining the peak amplitude.

17. The method of claim 15 further comprising providing each amplification stage with an operational amplifier configured in an inverting amplification mode.