Cloning, expression and characterization of a novel form of phosphatidylinositol-3-kinase

• US 5,885,777 A
• Filed: 04/11/1997
• Issued: 03/23/1999
• Est. Priority Date: 10/13/1994
• Status: Expired due to Term

• Current Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Original Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Inventors: Wetzker, Reinhard, Hanck, Theodor, Stoyanov, Borislav
• Primary Examiner(s): Chambers, Jasemine C.
• Assistant Examiner(s): Priebe, Scott D.
• Application Number:
  US08/817,090
• Time in Patent Office: 711 Days
• Field of Search: 435/6, 435/320.1, 435/172.3, 435/325, 435/252.3, 435/254.11, 435/254.2, 514/44, 514/23.2, 536/23.1, 536/23.5, 536/24.5, 536/24.31, 536/24.3
• US Class Current: 435/6.13
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Abstract

The present invention concerns a new phosphatidyl-inositol-3-kinase (PI3Kγ), a nucleic acid coding for it, an antibody directed against the protein as well as the diagnostic and therapeutic use of the protein, the nucleic acid and the antibody.

Claims

1. An isolated nucleic acid comprising:

(a) a nucleotide sequence encoding an active phosphatidylinositol-3-kinase consisting of the amino acid sequence set forth in SEQ ID NO;

2;

(b) a nucleotide sequence encoding an active phosphatidylinositol-3-kinase consisting of the amino acid sequence set forth in SEQ ID NO;

4;

or

(c) a nucleotide sequence which hybridizes with the nucleotide sequence of (a) or (b) after washing at 55°

C. in an aqueous low salt buffer containing 0.2×

SSC or 0.1% SDS.

2. The nucleic acid of claim 1, wherein the nucleic acid is a recombinant DNA molecule.

3. The nucleic acid of claim 1, wherein the nucleic acid comprises a nucleotide sequence consisting of:

(a) the protein-coding sequence set forth in nucleotides 423-3569 of SEQ ID NO;

1;

(b) the protein-coding sequence set forth in nucleotides 423-3572 of SEQ ID NO;

3;

or

(c) a sequence which hybridizes with the protein-coding sequence of (a) or (b) after washing at 55°

C. in an aqueous low salt buffer containing 0.2×

SSC or 0.1% SDS.

4. The nucleic acid of claim 1, wherein the nucleic acid comprises a nucleotide sequence which hybridizes with the protein-coding sequence of (a) or (b) after washing at 62°

C. in an aqueous low salt buffer containing 0.2×

SSC or 0.1% SDS.

5. The nucleic acid of claim 1, wherein the nucleic acid comprises a nucleotide sequence which hybridizes with the protein-coding sequence of (a) or (b) after washing at 68°

C. in an aqueous low salt buffer containing 0.2×

SSC or 0.1% SDS.

7. A vector, comprising the nucleic acid of one of claims 1-6.

8. A transformed cell, containing the nucleic acid of one of the claims 1-6.

9. A transformed cell, containing the vector of claim 7.

10. A method for determining a phosphatidylinositol-3-kinase mRNA produced in cells, comprising incubating a nucleic acid probe comprising a phosphatidylinositol-3-kinase-mRNA-specific region of the nucleic acid of claim 1 with the phosphatidylinositol-3-kinase mRNA and determining any binding between the probe and the mRNA.

11. The method of claim 10, wherein the cells are human cells.

6. An isolated nucleic acid comprising a nucleotide sequence of at least 20 consecutive nucleotides of the nucleotide sequence of SEQ ID NO:

1 or SEQ ID NO;

3, wherein the nucleotide sequence is specific for nucleic acid encoding phosphatidylinositol-3-kinase γ

.