Method of identifying analytes

• US 5,885,844 A
• Filed: 08/05/1996
• Issued: 03/23/1999
• Est. Priority Date: 09/21/1993
• Status: Expired due to Fees

• Current Assignee: E2V Technologies PLC
• Original Assignee: EEV Ltd. (Teledyne Technologies Incorporated)
• Inventors: Freeman, Neville John, Weir, Donald James, May, Iain Peter
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US08/617,769
• Time in Patent Office: 960 Days
• Field of Search: 436/151, 436/183, 73/24.06
• US Class Current: 436/151
• CPC Class Codes: G01N 2291/0212 Binary gases
• CPC Class Codes: G01N 2291/0222 Binary liquids
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves

Abstract

A method of identifying an analyte, using a chemical sensor (e.g., a piezoelectric crystal oscillator) which produces an output frequency responsive to different analytes by a characteristic change in the output frequency. The sensor is exposed to a step change (to) in concentration of the analyte and the resulting frequency characteristic measured. An expression for this characteristic is derived consisting of two exponential functions (F, S), one (F) derived from the early part of the frequency characteristic and the other (S) from the latter part. The four parameters (A₂, A₃, A₄, A₅) resulting from the functions so obtained are characteristic of the particular analyte.

Claims

1. A method of identifying an analyte using a chemical sensor which is arranged to produce an oscillatory output signal and which responds selectively to different analytes by a characteristic change in the frequency of the output signal, comprising the steps of:

exposing the sensor to step changes in concentration of the analyte corresponding to an increase in the concentration of the analyte, and to a decrease in the concentration of the analyte;

measuring the sensor output frequency over a period following the respective step changes to provide frequency-time characteristics;

deriving expressions for the characteristics comprising a combination of exponential functions having characteristic parameters which are together indicative of the identity of the analyte; and

comparing the characteristic parameters with reference parameters to identify the analyte.

2. The method according to claim 1;

and further comprising the step of forming the sensor of a material having a reversible reaction with the analytes.

3. The method according to claim 2;

and further comprising the step of employing a plurality of additional chemical sensors exhibiting different frequency-time characteristics for the analyte, and the step of deriving, from the plurality of the additional sensors, additional characteristic parameters which are together indicative of the identity of the analyte.

4. The method according to claim 1;

and further comprising the step of determining said characteristic parameters by an iterative method.

5. The method according to claim 4, wherein the determining step is performed by employing the Levenburg-Marquadht algorithm.

6. The method according to claim 4;

and further comprising the steps of obtaining initial estimates of said characteristic parameters by assuming that a slope of an early part of each said characteristic is predominantly attributable to a first exponential function and that a slope of a latter part of each said characteristic is predominantly attributable to a second exponential function, and by determining estimates of the first and second exponential functions from point frequency measurements within the respective parts.

7. The method according to claim 1;

and further comprising forming said sensor by coating a piezo-electric element with a sensor material which interacts with analytes selectively.

8. The method according to claim 1, wherein the step change corresponding to the decrease in the concentration of the analyte is performed by replacing an analyte-carrying gas by a non-analyte-carrying gas.

9. The method according to claim 1, wherein the deriving step is performed to obtain a first set of the characteristic parameters during said increase in concentration, and a second set of the characteristic parameters during said decrease in concentration, said first set of the characteristic parameters being independent of the second set of the characteristic parameters.