Device for measurement of luminescence of a fluid sample

• US 5,888,454 A
• Filed: 02/09/1998
• Issued: 03/30/1999
• Est. Priority Date: 02/07/1997
• Status: Expired due to Term

• Current Assignee: Stratec Elektronik GmbH
• Original Assignee: Stratec Elektronik GmbH
• Inventors: Trump, Martin, Leistner, Hermann, Eppler, Jorg
• Primary Examiner(s): Le, Long V.
• Application Number:
  US09/020,987
• Time in Patent Office: 414 Days
• Field of Search: 422/52, 422/63, 422/65, 422/104, 436/172, 436/805, 436/809, 356/440, 356/244, 356/246, 250/361 C
• US Class Current: 422/52
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

The invention concerns a measuring device for carrying out of luminescence measurements of fluid samples, with a light detector (10) which detects the light entering an aperture opening (36) of a inlet aperture (18) and a positioning device for positioning one of a multiplicity of sample vessels (20) arranged on a micro-test plate (16 with respect to the aperture opening (36). In order to prevent the spill-over of light between the individual sample vessels (20), a transverse shield (42) is provided capable of being brought into engagement with the sample plate, which in the engagement position separates the measurement opening (24) of the sample vessel (20'"'"') found in the measurement position from the measurement openings of the adjacent sample vessels (20).

Claims

1. Measurement device for effecting luminescence measurements of fluid samples, comprising:

a sample plate including a plurality of sample vessels (20) having upper measurement openings (24) through which measurement light passes, said measurement openings having outer rim edges,

a positioning device (14, 14'"'"') for positioning one of said sample vessels (20) in a measurement position,

a transverse shield (42) including an inlet aperture (18) having an aperture opening (36), said transverse shield provided between said sample vessel (20) and a light detector (10),

a light detector (10) for detecting the light passing through the aperture opening (36) of an inlet aperture (18) of said transverse shield (42),

wherein when said transverse shield (42) is brought into engagement with the sample plate (16), it engages behind the outer rim edges of the measurement opening (24) of the sample vessel (20'"'"') found in the measurement position and separates it from the measurement openings (24) of the adjacent sample vessels (20).

2. Measurement device according to claim 1, wherein said light detector (10) is a photo-multiplier.

3. Measurement device according to claim 1, wherein said transverse shield (42) is provided with an inner step (46) which engages around the outer rim edges of the measurement opening (24) of the sample vessel (20'"'"') situated in the measurement position.

4. Measurement device according to claim 1, wherein the transverse shield (42) extends from the measurement opening (24) of the sample vessel (20'"'"') situated in the measurement position at least to the bordering rim edges of the measurement opening (24) of the adjacent sample vessel (20).

5. Measurement device according to claim 1, wherein the transverse shield (42) form fittingly engages in a recess (28) on an upper side of the sample plate (16).

6. Measurement device according to claim 1, wherein the transverse shield includes, on the side of a seating surface (44) facing the sample plate (16), a barrier projection (42) which projects beyond the inlet aperture (18) and surrounds the aperture opening (36).

7. Measurement device according to claim 6, wherein the shield projection (42) exhibits side flanks (50, 52) which are slanted at acute angles to each other.

8. Measurement device according to claim 1, wherein said sample plate is constructed as a micro-test-plate (16), on which in a matrix-form orientation cylindrical sample vessels (20) project with open side end sections (26) above a horizontal or planar connection surface (22) as sample vessels, and wherein the transverse shield (42) is formed essentially form-fitting to a recess defined by an interstitial area (28) between end sections (26) of one of the sample vessels (20'"'"') situated in the measurement position and the surrounding sample vessels (20).

9. Measurement device according to claim 1, wherein the transverse shield (42) carrying inlet aperture (18) is provided moveably between a position raised from the sample plate (16) and an engagement position.

10. Measurement device according to claim 1, wherein the positioning device (14, 14'"'"') includes a pivot device (14'"'"'), by means of which the transverse shield (42) in rigid connection with the inlet aperture (18), and optionally the light detector (10), is pivotable about a horizontal pivot axis (54) with respect to the sample plate (16).

11. Measurement device according to claim 10, wherein the pivot movement into the engagement position is carried out by means of a pull magnet (58) against the force of a return spring (64).

12. Measurement device according to claim 1, wherein the positioning device (14, 14'"'"') is comprised of an automatically operated displacement mechanism (14) operated by means of a drive unit (66), on which the sample plate (16) is mounted.

13. Measurement device for effecting luminescence measurements of fluid samples, comprising:

a sample plate including a plurality of sample vessels (20) having upper measurement openings (24) through which measurement light passes, said measurement openings having outer rim edges,

a positioning device (14, 14'"'"') for positioning one of said sample vessels (20) in a measurement position,

a transverse shield plate (74) having one side capable of being brought into engagement with said measurement openings of said sample plate (16), said transverse shield plate provided between said sample vessel (20) and a light detector (10),

a light detector (10) for detecting the light passing through an aperture opening (36) of an inlet aperture (18) of said transverse shield (42),

wherein said transverse shield plate (74) exhibits a number of apertures (76) corresponding to the sample vessels (20) of the sample plate (16), which apertures engage around and behind the outer rim edges of the measurement opening (24) of the sample vessel (20'"'"') found in the measurement position and separate it from the measurement openings (24) of the adjacent sample vessels (20).

14. Measurement device according to claim 13, wherein the sample plate is formed as a micro-test-plate (16) with ring-shaped end segments (26) of a cylindrical sample chamber encompassing sample vessels (20) following a horizontal surface (22), into which end segments the apertures of the shield plate (74) formed as concentric step apertures (76) engage.

15. Measurement device according to claim 14, wherein the apertures are formed as step bore holes (76), in a cross-section of which are adapted to form-fittingly engage the end segments (26) of the sample vessels (20).

16. Measurement device according to claim 13, wherein the shield plate (74) is pivotably provided as a cover plate on one of transport frames (82) for receiving the sample plate (16).

17. Measurement device according to claim 13, wherein the inlet aperture (18) lies flat upon an upper plan side (84) of the shield plate (74).

18. Measurement device according to claim 13, wherein the positioning device (14, 14'"'"') is comprised of an automatically operated displacement mechanism (14) operated by means of a drive unit (66), on which the sample plate (16) is mounted.