Recombinant PRRSV proteins, diagnostic kits and vaccines containing such recombinant PRRSV proteins
First Claim
1. An isolated protein from the causative virus of porcine reproductive and respiratory syndrome selected from the group consisting of proteins coded by ORFs 2 to 7 of the virus PRRS-Olot.
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Abstract
Recombinant proteins of the causative virus of porcine reproductive and respiratory syndrome (PRRS), corresponding to ORFs 2 to 7 of the PRRSV Spanish isolate (PRRS-Olot), have been produced in baculovirus expression system using Sf9 cell cultures as a permissive host. These recombinant proteins are suitable for the formulation of vaccines capable of efficaciously protecting porcine livestock from PRRS and for the preparation of diagnostic kits adequate for detection of anti-PRRSV antibodies as well as of PRRSV in a pig biological sample. This invention is of interest to Veterinary Medicine.
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52 Claims
- 1. An isolated protein from the causative virus of porcine reproductive and respiratory syndrome selected from the group consisting of proteins coded by ORFs 2 to 7 of the virus PRRS-Olot.
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3. An isolated protein from the causative virus of porcine reproductive and respiratory syndrome comprising at least one amino acid sequence selected from the group consisting of the amino acid sequences depicted in SEQ ID NO:
- 2 through SEQ ID NO;
7. - View Dependent Claims (4, 5, 6, 7)
- 2 through SEQ ID NO;
- 8. An isolated nucleotide sequence comprising at least one ORF selected from the group consisting of ORFs 2 to 7 of the virus PRRS-Olot.
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28. A method of expressing isolated viral subunit proteins from the causative agent of porcine reproductive and respiratory syndrome of the virus PRRS-Olot comprising the steps of:
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i) inserting at least one isolated nucleotide sequence selected from the group consisting of ORFs 2 to 7 of PRRS-Olot into suitable transfer vectors; ii) transfecting permissive host cells with the transfer vectors so obtained; and iii) selecting for recombinant expression systems that express the protein encoded by the corresponding ORF. - View Dependent Claims (29, 30, 31, 32, 33, 34)
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- 35. A vaccine comprising at least one isolated protein encoded by any of ORFs 3, and 5 of PRRS-Olot and a suitable carrier.
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51. A diagnostic kit for the detection of the presence of antibodies that specifically identify PRRSV in a biological sample comprising at least one isolated protein encoded by any of ORFs 2 to 7 of PRRS-Olot and suitable detection means.
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52. A diagnostic kit for the detection of the presence of PRRSV in a biological sample comprising antibodies to at least one isolated protein encoded by ORFs 2 to 7 of PRRS-Olot and a suitable detection means.
Specification