Tumor diagnosis and prognosis

• US 5,888,746 A
• Filed: 03/10/1997
• Issued: 03/30/1999
• Est. Priority Date: 06/10/1994
• Status: Expired due to Fees

• Current Assignee: The Institute Of Molecular And Cell Biology
• Original Assignee: The Institute Of Molecular And Cell Biology
• Inventors: Tabiti, Karim, Pallen, Catherine Jane
• Primary Examiner(s): HUFF, SHEELA JITENDRA
• Application Number:
  US08/750,352
• Time in Patent Office: 750 Days
• Field of Search: 435/7.1, 435/6.6, 435/7.7, 435/7.9-7.95, 435/960, 435/40.5, 435/40.52
• US Class Current: 435/7.1
• CPC Class Codes: G01N 2474/20 Immunohistochemistry assay
• CPC Class Codes: G01N 33/57446 of stomach or intestine

Abstract

A method for diagnosis or prognosis of a cancer is disclosed. The method comprises (i) detecting in a first biological sample protein tyrosine phosphate α (PTPα) or PTPα nucleic acid, and (ii) comparing the level of PTPα or PTPα nucleic acid in the first sample with the level in a second biological sample known to be from normal tissue. Any overexpression of PTPα or PTPα nucleic acid in the first sample compared to the second sample is indicative that the first sample is from a cancerous tissue.

Claims

1. A method for diagnosis or prognosis of a cancer, which method comprises

(i) detecting in a first biological sample protein tyrosine phosphatase α

(PTPα

) or PTPα

nucleic acid, and

(ii) comparing the level of PTPα

or PTPα

nucleic acid in the first sample with the level in a second biological sample known to be from normal tissue,

wherein any overexpression of PTPα

or PTPα

nucleic acid in the first sample compared to the second sample is indicative that the first sample is from a cancerous tissue.

2. The method according to claim 1, wherein the second biological sample is from a pool of normal tissue and is obtained by mixing samples from more than one subject.

3. The method according to claim 1, wherein the cancer is a colorectal carcinoma.

4. The method according to claim 1, wherein in step (i) the PTPα

or PTPα

nucleic acid is detected in situ in a tissue sample.

5. The method according to claim 1, wherein in step (i) the PTPα

or PTPα

nucleic acid is detected in a sample of isolated cellular protein or isolated cellular nucleic acid.

6. The method according to claim 1, wherein in step (i) the PTPα

is detected with an immunoglobulin specific for PTPα

.

7. The method according to claim 1, wherein in step (i) the PTPα

nucleic acid is detected with an oligonucleotide probe to the nucleic acid.