Monoclonal antibodies against human pancreatic islet cells

• US 5,888,813 A
• Filed: 11/07/1995
• Issued: 03/30/1999
• Est. Priority Date: 02/14/1991
• Status: Expired due to Fees

• Current Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Brandt, Michael, Albert, Winfried, Endl, Josef, Kientsch-Engel, Rosemarie, Richter, Wiltrud, Jungfer, Herbert, Scherbaum, Werner, Eiermann, Thomas
• Primary Examiner(s): Wortman, Donna C.
• Application Number:
  US08/554,796
• Time in Patent Office: 1,239 Days
• Field of Search: 435/7.1, 435/7.4, 435/7.5, 435/7.9, 435/70.21, 435/965, 435/338, 435/372.2, 424/85.8, 436/506, 436/548, 530/303, 530/413, 530/845, 530/388.15, 530/387.2
• US Class Current: 435/338
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C07K 16/40 against enzymes
• CPC Class Codes: C07K 16/4241 against anti-human or anti-animal Ig
• CPC Class Codes: C07K 2317/21 from primates, e.g. man

Abstract

The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc γ and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells.

The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.

Claims

1. Human monoclonal antibody which specifically binds to a human pancreatic islet cell, wherein said monoclonal antibody is of isotype IgG and is produced by a cell line selected from the group consisting of ECACC 90121401, ECACC 90121402, and ECACC 90121403.

2. Human monoclonal antibody which specifically binds to a human pancreatic islet cell, wherein said monoclonal antibody is of isotype IgG and wherein said monoclonal antibody binds to the epitope bound by a monoclonal antibody produced by a cell line selected from the group consisting of ECACC 90121401, ECACC 90121402, and ECACC 90121403.

3. Cell line which produces a human monoclonal antibody of class IgG which specifically binds to human islet cells, wherein said cell line is selected from the group consisting of ECACC 90121401, ECACC 90121402, and ECACC 90121403.

4. Process for isolating an islet cell antigen which specifically binds to a monoclonal antibody produced by a cell line selected from the group consisting of ECACC 90121401, ECAC 90121402, and ECACC 90121403, comprising contacting an immobilized monoclonal antibody produced by said cell line with a solution free of insoluble material obtained from a member of the group consisting of human pancreatic islet cell lysate, animal pancreatic islet cell lysate, human pancreatic homogenate, animal pancreatic homogenate, human brain homogenate, animal brain homogenate, human cerebellum homogenate and animal cerebellum homogenate to form a complex between antigen in said solution and said monoclonal antibody, and isolating said antigen from said complex.

5. Human monoclonal antibody which specifically binds to a human pancreatic islet cell wherein said monoclonal antibody is of isotype IgG and is produced by cell line DSM ACC 2017.

6. Human monoclonal antibody which specifically binds to a human pancreatic islet cell, wherein said monoclonal antibody is of isotype IgG and said monoclonal antibody binds to the epitope bound by a monoclonal antibody produced by a cell line DSM ACC 2017.

7. Cell line which produces a human monoclonal antibody of class IgG which specifically binds to human islet cells, wherein said cell line is DSM ACC 2017.

8. Process for isolating an islet cell antigen which specifically binds to a monoclonal antibody produced by cell line DSM ACC 2017, comprising contacting an immobilized monoclonal antibody produced by said cell line with a solution free of insoluble material obtained from a member of the group consisting of human pancreatic islet cell lysate, animal pancreatic islet cell lysate, human pancreatic homogenate, animal pancreatic homogenate, human brain homogenate, animal brain homogenate, human cerebellum homogenate and animal cerebellum homogenate to form a complex between antigen in said solution and said monoclonal antibody, and isolating said antigen from said complex.