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Oxidative method for attachment of glycoproteins or glycopeptides to surfaces of medical devices

  • US 5,891,506 A
  • Filed: 12/04/1997
  • Issued: 04/06/1999
  • Est. Priority Date: 08/09/1996
  • Status: Expired due to Term
First Claim
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1. A method of forming a coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the surface being suitable for contacting tissue, blood and other bodily fluids in or temporarily removed from a living mammalian subject, the method comprising the ordered steps of:

  • (a) combining a periodate with a biomolecule, the biomolecule comprising a 1,2 dihydroxy moiety and an amino acid residue for oxidizing the 1,2 dihydroxy moiety to form an aldehyde-functional material;

    (b) providing the medical device, the device having a suitable biomaterial forming the surface, an amine moiety being disposed on the surface;

    (c) combining the aldehyde-functional material with the amine moiety to bond the aldehyde-functional material to the amine moiety and thereby form an imine moiety; and

    (d) reacting the imine moiety with a reducing agent to form an amine linkage, the amine linkage immobilizing the biomolecule on the surface, the immobilized biomolecule forming the coating.

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