Compositions for cytochrome P450 biotransformation reactions

• US 5,891,696 A
• Filed: 08/01/1997
• Issued: 04/06/1999
• Est. Priority Date: 08/02/1996
• Status: Expired due to Term

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: PanVera Corp. (Vertex Pharmaceuticals Incorporated)
• Inventors: Shaw, Peter M., Thompson, David V., Lowery, Robert G.
• Primary Examiner(s): Hendricks, Keith D.
• Application Number:
  US08/904,376
• Time in Patent Office: 613 Days
• Field of Search: 435/189
• US Class Current: 435/189
• CPC Class Codes: C12N 9/004 Cytochrome-b5 reductase (1.6.2.2)

Abstract

A storable biotransformation mixture that can oxidize a drug, comprising a cytochrome P450 enzyme, an electron donor; and, a buffer solution. In a preferred embodiment the mixture also contains liposomes, cytochrome b₅ and NADPH-P450 reductase as the electron donor. The mixture is storable at a temperature less than 8° C. to for at least two weeks and is able to oxidize a drug after storage.

Claims

1. A biotransformation mixture that can be stored at a temperature less than 8°

C. for at least two weeks and is able to oxidize a drug after storage, comprising;

a) a cytochrome P450 enzyme;

b) an electron donor;

c) a lipid; and

,

d) a buffer solution.

2. The mixture of claim 1 further comprising cytochrome b₅.

3. The mixture of claim 1 wherein the lipid comprises a liposome.

4. The mixture of claim 1 wherein the electron donor comprises a reduced puridine dinucleotide.

5. The mixture of claim 4 wherein the reduced puridine dinucleotide comprises NADPH.

6. The mixture of claim 1 wherein the electron donor comprises a NADPH-P450 oxidoreductase,

7. The mixture of claim 6 wherein the electron donor comprises a human NADPH-P450 oxidoreductase selected from the group consisting of recombinant NADPH-450 and naturally occuring NADPH-P450.

8. The mixture of claim 1 wherein the cytochrome P450 is selected from the group consisting of:

CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A5, CYP4A9, CYP4A11, and CYP7.

9. The mixture of claim 7 wherein the cytochrome P450 comprises a CYP1A2 enzyme.

10. The mixture of claim 7 wherein the cytochrome P450 comprises a CYP2C9 enzyme.

11. The mixture of claim 7 wherein the cytochrome P450 comprises a CYP3A4 enzyme.

12. The mixture of claim 7 wherein the human cytochrome P450 is selected from the group consisting of recombinant cytochrome P450 and naturally occurring cytochrome P450.

13. The mixture of claim 3 wherein the liposome is selected from the group consisting of:

L-α

-dilauroyl-sn-glycero-3-phosphocholine, L-α

-diloleoyl-sn-glycero-3-phosphocholine, and L-α

-dilauroyl-sn-glycero-3-phosphoserine.

14. The mixture of claim 1 wherein the buffer solution comprises sufficient amounts of MgCl₂ and GSH to allow the mixture to oxidize a drug.

15. The mixture of claim 1 wherein such mixture may be frozen at a temperature less than -20°

C. for at least two months and is able to oxidize a drug upon thawing.

16. A biotransformation mixture that may be frozen at a temperature less than 0°

C. and is able to oxidize a drug upon thawing, comprising;

a) a cytochrome P450 enzyme;

b) an electron donor;

c) a lipid; and

,

d) a buffer solution.

17. The mixture of claim 16 wherein the cytochrome P450 is selected from the group consisting of:

CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A5, CYP4A9, CYP4A11, and CYP7.

18. The mixture of claim 17 wherein the cytochrome P450 comprises a human enzyme selected from the group consisting of recombinant cytochrome P450 and naturally occurring cytochrome P450.

19. The mixture of claim 18 wherein the electron donor comprises NADPH.

20. The mixture of claim 18 wherein the electron donor comprises a NADPH-P450 oxidoreductase.

21. The mixture of claim 20 wherein the lipid comprises a liposome.

22. The mixture of claim 21 wherein the liposome is selected from the group consisting of:

L-α

-dilauroyl-sn-glycero-3-phosphocholine, L-α

-diloleoyl-sn-glycero-3-phosphocholine, and L-α

-dilauroyl-sn-glycero-3-phosphoserine.