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Reagent for measuring reticulocytes and a method of measuring them

  • US 5,891,731 A
  • Filed: 04/14/1997
  • Issued: 04/06/1999
  • Est. Priority Date: 04/12/1996
  • Status: Expired due to Term
First Claim
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1. A reagent for measuring reticulocytes comprising at least one dye which specifically stains leukocytes so as to differentiate reticulocytes from leukocytes and at least one dye which specifically stains reticulocytes selected from the group consisting of formula (I), ##STR17## wherein R1 is a hydrogen atom or a lower alkyl group;

  • R2 and R3 are, the same or different, hydrogen atom, a lower alkyl or lower alkoxy group;

    R4 is hydrogen atom, an acyl or lower alkyl group;

    R5 is hydrogen atom or an optionally substituted lower alkyl group;

    Z is sulfur atom, oxygen atom, or carbon atom substituted with a lower alkyl group;

    n is 1 or 2; and

    X- is an anion, formula (II'"'"'), ##STR18## wherein B is selected from the group consisting of --(CH═

    CH)n'"'"' --CH═

    , where n'"'"' is 0, 1 or 2 and --CH═

    C(--R4 '"'"') --CH═

    ;

    C'"'"' is selected from the group consisting of ##STR19## X'"'"' is selected from the group consisting of O, S, Se and --C(CH3)2 ;

    Y'"'"' is selected from the group consisting of Cl, Br and I; and

    R1 '"'"', R4 '"'"', R5 '"'"', R6 '"'"' and R7 '"'"' are, the same or different and selected from the group consisting of a lower alkyl, a lower alkenyl and a halogenated lower alkyl; and

    formula (III'"'"'), ##STR20## wherein B, X'"'"', and Y'"'"'- are as in formula (II'"'"') and, R2 '"'"' and R3 '"'"', are the same or different and selected from the group consisting of a lower alkyl, a lower alkenyl and a halogenated lower alkyl.

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