Amyloid precursor-like protein and uses thereof

• US 5,891,991 A
• Filed: 08/06/1996
• Issued: 04/06/1999
• Est. Priority Date: 04/20/1992
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology, The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: Massachusetts Institute of Technology, The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Wasco, Wilma, Bupp, Keith, Solomon, Frank, Magendantz, Margaret, Tanzi, Rudolph
• Primary Examiner(s): Hutzell, Paula K.
• Assistant Examiner(s): BAKALYAR, HEATHER
• Application Number:
  US08/689,276
• Time in Patent Office: 973 Days
• Field of Search: 530/350, 530/300, 530/810, 424/184.1, 424/185.1, 424/193.1
• US Class Current: 530/300
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/4711 Alzheimer's disease; Amyloid plaque core protein
• CPC Class Codes: Y10S 530/81 Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier

Abstract

The present invention is directed to isolated amyloid precursor-like proteins (APLPs), nucleotide sequences coding for and regulating expression of these protein, antibodies directed against these proteins, and recombinant vectors and host cells containing the genetic sequences coding for and regulating the expression of these protein sequences. The invention is also directed to isolated genomic. DNA, cDNA anti-sense RNA, and RNA containing the protein sequence. Antibodies can be used to detect an APLP in biological specimens, including, for example, fluid, serum or tissue samples. APLP1 and APLP2 are candidate genes for late onset familial Alzheimer'"'"'s disease.

Claims

1. A recombinant amyloid precursor-like protein (APLP), human APLP2, comprising the amino acid sequence of FIG. 9 (SEQ ID NO:

4).

2. A peptide of the formula QQLRELQRH (SEQ ID NO:

1).

3. An immunogenic peptide fragment comprising the following polypeptide sequence, or subsequence thereof:

space="preserve" listing-type="equation">QQLRELQRH(SEQ ID NO;

1)

said sequence, or said subsequence, being immunogenic so as to induce the production of active antibodies specific to APLP1 (SEQ ID NO;

3).

4. The immunogenic peptide fragment of claim 3, coupled to an immunogenic carrier.

5. A recombinant amyloid precursor-like protein (APLP), mouse APLP1, comprising the amino acid sequence of FIG. 2 (SEQ ID NO:

3).