Receptor for oncostatin M

• US 5,891,997 A
• Filed: 04/10/1998
• Issued: 04/06/1999
• Est. Priority Date: 05/26/1994
• Status: Expired due to Term

• Current Assignee: Immunex Corporation (Amgen Inc.)
• Original Assignee: Immunex Corporation (Amgen Inc.)
• Inventors: Cosman, David J., Mosley, Bruce
• Primary Examiner(s): Caputa, Anthony
• Assistant Examiner(s): Gucker, Stephen
• Application Number:
  US09/058,263
• Time in Patent Office: 361 Days
• Field of Search: 435/69.1, 435/69.7, 424/178.1, 530/350
• US Class Current: 530/350
• CPC Class Codes: C07K 14/70503 Immunoglobulin superfamily
• CPC Class Codes: C07K 14/7155 for interleukins [IL]
• CPC Class Codes: C07K 16/248 IL-6
• CPC Class Codes: C07K 16/2866 against receptors for cytokines, lymphokines, interferons
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
• CPC Class Codes: G01N 33/6863 Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors

Abstract

A novel polypeptide functions as the β chain of an oncostatin M receptor and is thus designated OSM-Rβ. Heterodimeric receptor proteins comprising OSM-Rβ and gp130 bind oncostatin M and find use in inhibiting biological activities mediated by oncostatin M.

Claims

1. A purified polypeptide encoded by a DNA selected from the group consisting of:

a) DNA comprising nucleotides 368 to 3304 of SEQ ID NO;

5 or nucleotides 449 to 3304 of SEQ ID NO;

5;

b) DNA capable of hybridizing to the DNA of (a), under highly stringent conditions that include hybridization at 68°

C. followed by washing in 0.1×

SSC/0.1%SDS at 63°

-68°

C.; and

encoding a polypeptide; and

c) DNA that encodes the amino acid sequence presented in SEQ ID NO;

6.

2. An OSM-Rβ

polypeptide according to claim 1, wherein said OSM-Rβ

is a soluble OSM-Rβ

polypeptide.

3. A soluble OSM-Rβ

according to claim 2, comprising amino acids -27 to x of SEEQ ID NO;

6 or 1 to x of SEQ ID NO;

6, wherein x is an integer between 432 and 714, inclusive.

6. An OSM-Rβ

polypeptide according to claim 1, wherein said OSM-Rβ

is encoded by the OSM-Rβ

cDNA in the recombinant vector deposited in strain ATCC 69675.

7. A soluble OSM-Rβ

polypeptide according to claim 2, wherein said polypeptide additionally comprises an Fc polypeptide fused to the C-terminus of said OSM-Rβ

polypeptide.

4. A purified OSM-Rβ

polypeptide, wherein said OSM-Rβ

polypeptide comprises the amino acid sequence Glu Arg Leu Pro Leu Thr Pro Val Ser Leu Lys Val (amino acid residues 1-12 of SEQ ID NO;

6), wherein said amino acid sequence is the N-terminal of a purified OSM-Rβ

polypeptide.

5. An OSM-Rβ

polypeptide comprising amino acids 1 to 952 of SEQ ID NO;

6.