Immunoassay for the detection of ligands
First Claim
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1. An immunoassay for detecting a ligand comprising:
- a) immobilizing a ligand-specific binding material onto a solid phase;
b) reacting the solid phase with a test sample;
c) reacting the solid phase with a biotin-labeled ligand-specific binding material;
d) reacting the solid phase with antibiotin labeled with a marker;
e) separating unreacted reagents from the solid phase; and
f) measuring the amount of the marker in the solid phase or in the unreacted reagents to detect the amount of ligand present in the sample.
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Abstract
A biotin-antibiotin immunoassay comprises coating a solid phase with a ligand-specific binding material, reacting the solid phase with a test sample, reacting the solid phase with the biotin-labeled form of the ligand-specific binding material and antibiotin labeled with a suitable marker. The marker is then measured to detect the amount of ligand present in the test sample.
69 Citations
21 Claims
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1. An immunoassay for detecting a ligand comprising:
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a) immobilizing a ligand-specific binding material onto a solid phase; b) reacting the solid phase with a test sample; c) reacting the solid phase with a biotin-labeled ligand-specific binding material; d) reacting the solid phase with antibiotin labeled with a marker; e) separating unreacted reagents from the solid phase; and f) measuring the amount of the marker in the solid phase or in the unreacted reagents to detect the amount of ligand present in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. An immunoassay for detecting a ligand comprising:
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a) immobilizing a ligand-specific binding material onto a solid phase; b) reacting the solid phase with a human biological fluid; c) reacting the solid phase with a biotin-labeled ligand; d) reacting the solid phase with an enzyme-labeled antibiotin; e) separating unreacted reagents from the solid phase; and f) measuring amount of the enzyme in the solid phase or in the unreacted reagents to detect the amount of ligand present in the human biological fluid. - View Dependent Claims (12, 13)
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14. An immunoassay for detecting a ligand which may be present in a biological fluid test sample, comprising:
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(a) reacting the test sample with a solid phase to which a ligand-specific binding material has been immobilized; (b) reacting said solid phase with a biotin-labeled specific binding material; (c) reacting said solid phase with antibiotin labeled with a marker; (d) separating unreacted reagents from the solid phase; (e) measuring the amount of the marker in the solid phase or in the unreacted reagents to detect the amount of ligand present in the sample. - View Dependent Claims (15, 16, 17, 18)
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19. A competitive immunoassay for detecting a ligand which may be present in a biological fluid sample, comprising:
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(a) reacting the test sample with a solid phase to which a ligand-specific binding material has been attached, a biotin-labeled ligand and antibiotin labeled with a marker; (b) separating unreacted reagents from the solid phase; (c) measuring the amount of the marker in the solid phase or in the unreacted reagents to detect the amount of ligand present in the sample. - View Dependent Claims (20, 21)
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Specification