Controlled sheath flow injection cytometry
First Claim
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1. A method for flow cytometry comprising the steps of:
- a) delivering a rapid sheath flow through a flow cell of a flow cytometer at an initial flow rate;
b) rapidly mixing a sample and a reactant outside said flow cell for delivery to said flow cell;
c) reducing flow of said sheath through said flow cell to a lower flow rate;
d) delivering mixed sample and reactant through said flow cell during the time when the flow of said sheath is reduced;
e) redelivering said rapid sheath flow through said flow cell at said initial flow rate; and
f) measuring a cell fluorescence of said mixed sample and reactant;
whereby focused measurement of said sample is obtained due to reduced turbulent flow of sheath, sample and reactant.
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Abstract
A flow cytometry method and system to regulate both the sheath and sample streams during the period of sample injection. The period of turbulence between the streams is reduced to approximately 100 milliseconds thereby allowing for focused measurement of cell associated fluorescence.
95 Citations
7 Claims
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1. A method for flow cytometry comprising the steps of:
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a) delivering a rapid sheath flow through a flow cell of a flow cytometer at an initial flow rate; b) rapidly mixing a sample and a reactant outside said flow cell for delivery to said flow cell; c) reducing flow of said sheath through said flow cell to a lower flow rate; d) delivering mixed sample and reactant through said flow cell during the time when the flow of said sheath is reduced; e) redelivering said rapid sheath flow through said flow cell at said initial flow rate; and f) measuring a cell fluorescence of said mixed sample and reactant;
whereby focused measurement of said sample is obtained due to reduced turbulent flow of sheath, sample and reactant. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Current AssigneeNew Mexico State University
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Original AssigneeThe University of New Mexico
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InventorsSeamer, Larry C., Sklar, Larry A.
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Primary Examiner(s)WALLENHORST, MAUREEN
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Application NumberUS08/976,637Time in Patent Office512 DaysField of Search436/52, 436/53, 436/63, 436/164, 436/172, 356/317, 356/338, 422/81, 422/82, 422/82.05, 422/82.08US Class Current436/63CPC Class CodesG01N 15/1404 Handling flow, e.g. hydrody...Y10T 436/117497 with a continuously flowing...Y10T 436/118339 with formation of a segment...
