Method for sample injection in microchannel device
First Claim
1. A method for transporting a liquid sample containing at least one sample material into a third microchannel from an intersection of said third microchannel with at least a first, a second, and a fourth microchannel, comprising:
- in a first stage, moving liquid sample in and from said fourth microchannel through said intersection and into said second microchannel and concurrently moving carrier liquid in and from said first microchannel and in and from said third microchannel through said intersection and into said second microchannel;
thereafterin a second stage, moving at least part of the contents of said intersection into said third microchannel and concurrently moving a part of the contents of said second and fourth microchannels through said intersection and into said third microchannel, whereby a quantity of said sample material is moved into said third microchannel; and
thereafterin a third stage, moving carrier liquid from said first microchannel simultaneously through said intersection and into said second, third, and fourth microchannels.
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Accused Products
Abstract
A method and device are provided for transporting a liquid sample into a third microchannel from an intersection of at least a first, a second, and a fourth microchannel, by stages. In a first stage, liquid sample is moved in and from the fourth microchannel through the intersection and into the second microchannel and concurrently carrier liquid is moved in and from the first and third microchannels through the intersection and into the second microchannel. Thereafter in a second stage, at least part of the contents of the intersection is moved into the third channel and concurrently a part of the contents of the second and fourth microchannels is moved through the intersection and into the third microchannel. Thereafter in a third stage, carrier liquid is moved from the first microchannel simultaneously through the intersection and into the second, third, and fourth microchannels. In some embodiments the liquid sample and the carrier liquid are moved electrokinetically, that is, by application of an electric field to segments of the microchannels.
365 Citations
18 Claims
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1. A method for transporting a liquid sample containing at least one sample material into a third microchannel from an intersection of said third microchannel with at least a first, a second, and a fourth microchannel, comprising:
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in a first stage, moving liquid sample in and from said fourth microchannel through said intersection and into said second microchannel and concurrently moving carrier liquid in and from said first microchannel and in and from said third microchannel through said intersection and into said second microchannel;
thereafterin a second stage, moving at least part of the contents of said intersection into said third microchannel and concurrently moving a part of the contents of said second and fourth microchannels through said intersection and into said third microchannel, whereby a quantity of said sample material is moved into said third microchannel; and
thereafterin a third stage, moving carrier liquid from said first microchannel simultaneously through said intersection and into said second, third, and fourth microchannels. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for transporting a liquid sample containing at least one sample material into a third microchannel from an intersection of said third microchannel with at least a first, a second, and a fourth microchannel, comprising:
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in a first stage, moving liquid sample in and from said fourth microchannel through said intersection and into said second microchannel and concurrently moving carrier liquid in and from said first microchannel and in and from said third microchannel through said intersection and into said second microchannel;
thereafterin a second stage, moving at least part of the contents of said intersection into said third microchannel and concurrently moving a part of the contents of said second, fourth and first microchannels through said intersection and into said third microchannel, whereby a quantity of said sample material is moved into said third microchannel; and
thereafterin a third stage, moving carrier liquid from said first microchannel simultaneously through said intersection and into said second, third, and fourth microchannels. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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Specification