Biosensor and method for quantitative measurement of a substrate using the same
First Claim
1. A biosensor comprising an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed on or in the vicinity of said electrode system and contains at least an oxidoreductase and an electron mediator, said counter electrode including at least a reductant of a redox compound or a metal permitting electrolytic oxidation.
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Abstract
A biosensor is disclosed for application to a method for quantitative measurement of a substrate comprising a first step of causing a substrate contained in a sample to react with an oxidoreductase specific to the substrate in the presence of an electron mediator in oxidized state, a second step of applying a potential to a working electrode for reducing the electron mediator in oxidized state that remains not reduced in the first step, and a third step of measuring a reduction current flowing across the working electrode and a counter electrode. The biosensor comprises an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on the base plate, and a reaction layer which is formed on or in the vicinity of the electrode system and contains at least an oxidoreductase and an electron mediator, the counter electrode including at least a reductant of a redox compound or an electrolytically oxidizable metal.
305 Citations
6 Claims
- 1. A biosensor comprising an electrically insulating base plate, an electrode system having a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed on or in the vicinity of said electrode system and contains at least an oxidoreductase and an electron mediator, said counter electrode including at least a reductant of a redox compound or a metal permitting electrolytic oxidation.
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6. A method for quantitative measurement of a substrate by using a biosensor which comprises an electrically insulating base plate, an electrode system having a working electrode and a counter electrode including at least a reductant of a redox compound and an electrically oxidizable metal, both being formed on said base plate, and a reaction layer which is formed on or in the vicinity of said electrode system and contains at least an oxidoreductase and an electron mediator, said method comprising:
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a first step of adding a sample to said reaction layer to cause a substrate contained in said sample to react with an enzyme contained in said reaction layer, a second step of applying a potential to said working electrode for reducing said electron mediator in oxidized state that remains not reduced in the course of said first step, and a third step of measuring a reduction current flowing across said working electrode and said counter electrode.
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Specification