Heated reaction chamber in a unified fluid circuit of a hematology diagnostic instrument

• US 5,908,599 A
• Filed: 07/30/1996
• Issued: 06/01/1999
• Est. Priority Date: 07/30/1996
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Bayer Corporation (Bayer AG)
• Inventors: Carlucci, Joseph, Catania, Loretta, Behringer, Bruce E.
• Primary Examiner(s): ACHUTAMURTHY, PONNATHAPURA N
• Application Number:
  US08/688,518
• Time in Patent Office: 1,036 Days
• Field of Search: 422/50, 422/63, 422/68.1
• US Class Current: 422/50
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K

Abstract

An integral heated reaction chamber for use in a unified fluid circuit having an acrylic body. The acrylic body is preferably formed of first and second acrylic layers bonded or fused together. A chamber half is formed in each of the acrylic layers and the layers are bonded together in opposition. A thinning notch is provided in one of the acrylic layers to form a thin wall of the chamber, and a heater element is secured adjacent the thin wall to heat the contents of the chamber. A temperature sensor is provided to monitor the temperature of the chamber contents. The sensed temperature may be used to control the heating element and the heating time according to a predetermined reaction protocol.

Claims

1. In a unified fluid circuit having a first acrylic layer and a second acrylic layer having a common interface there between, an integral heated reaction chamber comprising:

a hollow surface formed in one of the first and second acrylic layers at the common interface to form a chamber containing a volume;

a thinning notch formed in at least one of the first and second layers proximate to the chamber, defining a thin wall of said chamber bounded on a first side by said chamber and on a second side by the thinning notch; and

a heating element in thermal contact with said second side of said thin wall.

2. The reaction chamber of claim 1 further comprising a sensor operable to determine the temperature in the volume of the chamber.

3. The reaction chamber of claim 2 further comprising a bore extending from said chamber to an external surface of one of said first and second acrylic layers;

and wherein the sensor is disposed through the bore into the cavity.

4. The reaction chamber of claim 3 wherein the temperature sensor further comprises one of a thermocouple and a thermistor probe.

5. The reaction chamber of claim 3 wherein the heating element further comprises a foil heating element in contact with the second side of said thin wall.

6. The reaction chamber of claim 1 wherein the heating element further comprises a wire resistor in thermal contact with the second side of the thin wall.

7. The reaction chamber of claim 1 wherein the volume of the chamber is in a range of between 1000 and 2500 μ

l.

8. The reaction chamber of claim 1 wherein the chamber comprises a generally cylindrical cavity.

9. The reaction chamber of claim 1 further comprising a fluid inlet passage in fluid communication with the chamber.

10. The reaction chamber of claim 1 wherein the chamber further comprises first and second hollow chambers respectively formed in the first and second acrylic layers at the common interface.

11. The reaction chamber of claim 10 wherein the chamber comprises a generally cylindrical cavity.

12. The reaction chamber of claim 11 wherein the fluid passage intersects the chamber at a tangent to the chamber volume.

13. The reaction chamber of claim 10 wherein the chamber comprises a generally cylindrical cavity having a radius and a longitudinal axis, and the fluid inlet passage intersects the chamber at a first angle relative to the longitudinal cavity and a second angle relative to the radius.

14. The reaction chamber of claim 1 wherein the heating element is operable to heat any contents of the reaction temperature to a temperature in the range of from 32°

C. to 35°

C.

15. The reaction chamber of claim 1 further comprising a third acrylic layer fixed in contact connected to the one acrylic layer containing the thinning notch on a side opposite said chamber, wherein the heating element is disposed between the one and third acrylic layers.

16. The reaction chamber of claim 1, wherein the thin wall has a thickness of less than approximately 1.0 mm.

17. In a unified fluid circuit, an integral reaction chamber comprising:

an acrylic body;

a cavity formed in the acrylic body, the cavity defining a reaction chamber;

a thinning notch formed in the acrylic body proximate to said cavity and defining a thin wall of said reaction chamber bounded on a first side by the cavity and on a second side by the thinning notch; and

a heating element in thermal contact with said second side of said thin wall.

18. The reaction chamber of claim 17 further comprising a sensor operable to determine the temperature in the volume of the chamber.

19. The reaction chamber of claim 18 further comprising a bore through the acrylic body wherein the sensor comprises a component passing through the bore into said cavity.

20. The reaction chamber of claim 17 wherein the cavity further comprises a cylindrical section having a top end and a bottom end, and wherein the acrylic body further comprises an inlet fluid passage opening into said cavity at the bottom end of the cylindrical section of the cavity.

21. The reaction chamber of claim 17 wherein the acrylic body further comprises a first acrylic layer and a second acrylic layer fused together, the cavity being formed in opposing surfaces of the first and second acrylic layers.

22. The reaction chamber of claim 17 wherein the thin wall has a thickness of less than 1.0 mm.