Compositions and methods for the delivery of biologically active molecules using genetically altered cells contained in biocompatible immunoisolatory capsules
First Claim
1. A method for delivering a neurotrophic factor to the striatum of a mammalian host, the method comprising implanting at least one biocompatible capsule into the striatum of the host, each capsule comprising(a) a core comprising living mammalian cells secreting said neurotrophic factor, the cells having been transfected with a recombinant DNA molecule comprising a DNA sequence encoding said neutrotrophic factor operably linked to a promoter and wherein said cells stably express said neurotrophic factor;
- and(b) a biocompatible jacket surrounding the core, the jacket comprising a permselective membrane permitting passage of the neurotrophic factor thereacross into the host.
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Abstract
This invention provides improved devices and methods for long-term, stable expression of a biologically active molecule using a biocompatible capsule containing genetically engineered cells for the effective delivery of biologically active molecules to effect or enhance a biological function within a mammalian host. The novel capsules of this invention are biocompatible and are easily retrievable. This invention specifically provides improved methods and compositions which utilize cells transfected with recombinant DNA molecules comprising DNA sequences coding for biologically active molecules operatively linked to promoters that are not subject to down regulation in vivo upon implantation into a mammalian host. Furthermore, the methods of this invention allow for the long-term, stable and efficacious delivery of biologically active molecules from living cells to specific sites within a given mammal. In addition, this invention provides a general means for maintaining, for extended periods of time, the in vivo expression of transgenes.
59 Citations
9 Claims
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1. A method for delivering a neurotrophic factor to the striatum of a mammalian host, the method comprising implanting at least one biocompatible capsule into the striatum of the host, each capsule comprising
(a) a core comprising living mammalian cells secreting said neurotrophic factor, the cells having been transfected with a recombinant DNA molecule comprising a DNA sequence encoding said neutrotrophic factor operably linked to a promoter and wherein said cells stably express said neurotrophic factor; - and
(b) a biocompatible jacket surrounding the core, the jacket comprising a permselective membrane permitting passage of the neurotrophic factor thereacross into the host. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification