Transcription factor regulating MHC expression CDNA and genomic clones encoding same and retroviral expression constructs thereof
First Claim
1. A chimeric DNA molecule comprising a DNA sequence that encodes a polypeptide having a sequence corresponding to SEQ ID NO:
- 5, the sequence of said polypeptide selected from the group consisting of SEQ ID NO;
5, muteins of SEQ ID NO;
5, truncations of SEQ ID NO;
5, and fusion proteins containing them, wherein said polypeptide specifically binds to double stranded DNA having the sequence of SEQ ID NO;
3.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to NF-X1, a novel DNA binding protein which regulates expression of major histocompatibility complex (MHC) class II molecules, and to DNA sequences which encode the protein as well as recombinant expression of the protein. NF-X1 is a newly identified, cysteine-rich polypeptide which interacts sequence-specifically with the conserved X1 box regulatory element found in the proximal promoters of class II MHC genes. A cysteine-rich domain within NF-X1 contains a motif repeated seven times, and this entire region is necessary and sufficient for both sequence specific binding and effector function. The motif is related to but distinct from the previously described metal-binding protein families: LIM domain and RING finger. NFX.1 mRNA is markedly overexpressed late after induction of cells with interferon-gamma, and this overexpression coincides with a reduction in the level of HLA-DRA transcript in these cells. Overexpression of this protein strongly and specifically represses the transcription of the HLA-DRA gene in MHC class II positive cell lines, indicating that the NF-X1 protein is a transcriptional repressor of MHC class II molecules.
13 Citations
11 Claims
-
1. A chimeric DNA molecule comprising a DNA sequence that encodes a polypeptide having a sequence corresponding to SEQ ID NO:
- 5, the sequence of said polypeptide selected from the group consisting of SEQ ID NO;
5, muteins of SEQ ID NO;
5, truncations of SEQ ID NO;
5, and fusion proteins containing them, wherein said polypeptide specifically binds to double stranded DNA having the sequence of SEQ ID NO;
3. - View Dependent Claims (2, 3, 4, 5, 6, 11)
- 5, the sequence of said polypeptide selected from the group consisting of SEQ ID NO;
- 7. A DNA molecule comprising an intron-free DNA sequence encoding the amino acid sequence of SEQ ID NO:
-
10. A single stranded DNA molecule comprising at least 20 sequential nucleotides, wherein said sequential nucleotides comprise a subsequence found in SEQ ID NO:
- 4 or a DNA sequence complementary thereto.
Specification