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Coupled amplification and ligation method

  • US 5,912,148 A
  • Filed: 09/19/1996
  • Issued: 06/15/1999
  • Est. Priority Date: 08/19/1994
  • Status: Expired due to Term
First Claim
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1. A method for detecting a target polynucleotide in a sample comprising:

  • amplifying the target polynucleotide by primer extension to form an amplification product that is complementary to the target polynucleotide, the amplification being performed (a) in the presence of at least one pair of oligonucleotide probes capable of hybridizing to contiguous sequences of the amplification product; and

    (b) at a higher temperature than a melting point temperature of the oligonucleotide probes;

    ligating those oligonucleotide probes which hybridize to contiguous sequences of the amplification product to form a ligation product; and

    detecting the ligation product.

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