Coupled amplification and ligation method

• US 5,912,148 A
• Filed: 09/19/1996
• Issued: 06/15/1999
• Est. Priority Date: 08/19/1994
• Status: Expired due to Term

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Perkin-Elmer Corporation Applied Biosystems
• Inventors: Eggerding, Faye
• Primary Examiner(s): Sisson, Bradley L.
• Application Number:
  US08/975,902
• Time in Patent Office: 999 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 435/183, 435/810, 436/94, 436/800, 536/23.1, 536/23.5, 536/24.31, 536/24.33, 536/25.3, 536/25.32, 536/24.3, 935/5, 935/8, 935/76, 935/77
• US Class Current: 435/91.2
• CPC Class Codes: C12Q 1/6846 Common amplification features
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 1/6862 Ligase chain reaction [LCR]
• CPC Class Codes: C12Q 2527/107 Temperature of melting, i.e. Tm
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2533/10 the purpose being to increase the length of an oligonucleotide strand
• CPC Class Codes: C12Q 2547/101 by confinement to a single tube/container
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

A method based on polymerase chain reaction (PCR) amplification and oligonucleotide ligase assay (OLA) reaction is provided for analyzing complex genetic systems in a single reaction vessel. The method involves simultaneously incubating a sample containing one or more target polynucleotides with PCR primers and OLA probes in a single reaction mixture. The presence of variant polynucleotide sequences in the sample is determined by detecting and identifying the products of the OLA reaction.

Claims

1. A method for detecting a target polynucleotide in a sample comprising:

amplifying the target polynucleotide by primer extension to form an amplification product that is complementary to the target polynucleotide, the amplification being performed (a) in the presence of at least one pair of oligonucleotide probes capable of hybridizing to contiguous sequences of the amplification product; and

(b) at a higher temperature than a melting point temperature of the oligonucleotide probes;

ligating those oligonucleotide probes which hybridize to contiguous sequences of the amplification product to form a ligation product; and

detecting the ligation product.

2. The method according to claim 1 wherein the at least one pair of oligonucleotide probes include a first oligonucleotide probe and a second oligonucleotide probe which hybridizes to the target polynucleotide 3'"'"' relative to the first oligonucleotide probe, the second oligonucleotide probe including a moiety at a 3'"'"' end which prevents extension of the second oligonucleotide probe by a polymerase.

3. The method according to claim 2 wherein the moiety which prevents extension of the second oligonucleotide probe is a detectable marker.

4. The method according to claim 1 wherein the at least one pair of oligonucleotide probes include a first oligonucleotide probe and a second oligonucleotide probe which hybridizes to the target polynucleotide 3'"'"' relative to the first oligonucleotide probe, the first oligonucleotide probe including a moiety attached to a 5'"'"' end which does not hybridize to the amplification product and modifies the mobility of the ligation product in a size dependent separation process, the method further including the step of isolating the ligation product by a size dependent separation process.

5. The method according to claim 4 wherein the mobility modifier is a polymer chain.

6. The method of claim 1 wherein the step of amplifying is performed at a temperature between about 72°

C. and about 84°

C. and the step of ligating is performed at a temperature between about 30°

C. and about 55°

C.

7. The method of claim 1 wherein the step of amplifying is performed at a temperature between about 72°

C. and about 75°

C. and the step of ligating is performed at a temperature between about 40°

C. and about 55°

C.

8. A method for detecting a plurality of target polynucleotides in a sample comprising:

amplifying the plurality of target polynucleotides by primer extension to form a plurality of amplification products that are complementary to the plurality of target polynucleotides, the amplification being performed (a) in the presence of a plurality of pairs of oligonucleotide probes capable of hybridizing to contiguous sequences of the amplification products, and (b) at a higher temperature than a melting point temperature of the oligonucleotide probes;

ligating those oligonucleotide probes which hybridize to contiguous sequences of the plurality of amplification products to form a plurality of ligation products; and

detecting the plurality of ligation products.

9. The method of claim 8 wherein the plurality of ligation products each have a different fluorescent label, and the step of detecting includes detecting a fluorescent signal generated by the different fluorescent labels.

10. The method of claim 9 wherein the fluorescent labels are selected from the group consisting of 5-carboxyfluorescein, 6-carboxyfluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-6-carboxyfluorescein, N,N,N'"'"',N'"'"'-tetramethyl-6-carboxy rhodamine, 6-carboxyrhodamine X, 4,7,2'"'"',4'"'"',5'"'"',7'"'"'-hexachloro-6-carboxyfluorescein, 4,7,2'"'"',4'"'"',5'"'"',7'"'"'-hexachloro-5-carboxyfluorescein, 2'"'"',4'"'"',5'"'"',7'"'"'-tetrachloro-5-carboxyfluorescein, 4,7,2'"'"',7'"'"'-tetrachloro-6-carboxyfluorescein, 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4,7-dichloro-5-carboxyfluorescein, and 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4,7-dichloro-6-carboxyfluorescein.

11. The method of claim 8 wherein the plurality of ligation products each have a distinct electrophoretic mobility and the stop of detecting includes separating the plurality of ligation products by a size dependent separation process.

12. The method according to claim 11 wherein the plurality of pairs of oligonucleotide probes each include a first oligonucleotide probe and a second oligonucleotide probe which hybridizes to the target polynucleotide 3'"'"' relative to the first oligonucleotide probe, the first oligonucleotide probe including a moiety attached to a 5'"'"' end which does not hybridize to the amplification product and modifies the mobility of the ligation product in a size dependent separation process.

13. The method according to claim 12 wherein the mobility modifier is a polymer chain.

14. The method according to claim 13 wherein the polymer chain includes polyethylene oxide or polypropylene subunits.

15. The method of claim 12 wherein the plurality of ligation products each have a different fluorescent label, and the step of detecting includes detecting a fluorescent signal generated by the different fluorescent labels.

16. The method of claim 8 wherein the plurality of target polynucleotides are alleles or mutations of the gene encoding cystic fibrosis transmembrane conductance regulator (CFTR).

17. The method of claim 16 wherein the plurality of target polynucleotides are alleles or mutations of the gene encoding cystic fibrosis transmembrane conductance regulator (CFTR) selected from the group consisting of Δ

F518, G542X, G551D, W1282X, N1303K 3905insT, 3849+10kbCT, 3849+4AG, 3659delC, R117H, R1162X, 1717-1GA, 621+1GT, R553X, 2789+5GA, R347P, 2184delA, 1078delT, R334W, 711+1GT, G85E, 1898+1GA, A455E, S549R, S549N, R560T, Δ

l517, Q493X, V520F and Y122X.

18. The method of claim 8 wherein the step of amplifying is performed at a temperature between about 72°

C. and about 84°

C. and the step of ligating is performed at a temperature between about 30°

C. and about 55°

C.

19. The method of claim 8 wherein the step of amplifying is performed at a temperature between about 72°

C. and about 75°

C. and the step of ligating is performed at a temperature between about 40°

C. and about 55°

C.

20. A kit for detecting a target polynucleotide of known sequence in a sample comprising:

a sufficient quantity of amplification primers to perform an amplification reaction on the target polynucleotide to form an amplification product; and

a sufficient quantity of two isolated oligonucleotide probes capable of hybridizing to contiguous sequences of the amplification product to perform a ligation reaction on the oligonucleotide probes, the amplification primers having a melting point temperature sufficiently high to enable performance of the amplification reaction at a temperature above the melting point temperature of the oligonucleotide probes.

21. The kit according to claim 20 wherein the isolated amplification primers have a sequence of about 30-50 nucleotides and melting point temperature of at least 80°

C. and the isolated oligonucleotide probes have a sequence of about 8-30 nucleotides and a melting point temperature less than 70°

C.

22. The kit according to claim 20 wherein one of the oligonucleotide probes includes a fluorescent label.

23. The kit according to claim 20 where the two oligonucleotide probes include a first oligonucleotide probe and a second oligonucleotide probe which hybridizes to the target polynucleotide 3'"'"' relative to the first oligonucleotide probe, the second oligonucleotide probe including a moiety at a 3'"'"' end which prevents extension of the second oligonucleotide probe by the polymerase.

24. The kit according to claim 20 where the two oligonucleotide probes include a first oligonucleotide probe and a second oligonucleotide probe which hybridizes to the target polynucleotide 3'"'"' relative to the first oligonucleotide probe, the first oligonucleotide probe including a moiety attached to a 5'"'"' end which does not hybridize to the amplification product and modifies the mobility of the ligation product in a size dependent separation process.

25. The kit according to claim 24 wherein the mobility modifier is a polymer chain.

26. The kit according to claim 25 wherein the polymer chain includes polyethylene oxide or polypropylene subunits.

27. A kit for detecting a plurality of target polynucleotides of known sequence in a sample comprising:

sufficient quantities of amplification primers to perform amplification reactions on the plurality of target polynucleotides to form a plurality of amplification products; and

sufficient quantities of a plurality of pairs of isolated oligonucleotide probes capable of hybridizing to contiguous sequences of the plurality of amplification products to perform ligation reactions on the pairs of oligonucleotide probes, each amplification primer having a melting point temperature sufficiently high to enable performance of the amplification reactions at a temperature above a melting point temperature of the oligonucleotide probes.

28. The kit according to claim 27 wherein the isolated amplification primers have a sequence of about 30-50 nucleotides and a melting point temperature of at least 80°

C. and the isolated oligonucleotide probes have a sequence of about 8-30 nucleotides and a melting point temperature less than 70°

C.

29. The kit according to claim 27 where the pairs of oligonucleotide probes include a first oligonucleotide probe and a second oligonucleotide probe which hybridizes to the target polynucleotide 3'"'"' relative to the first oligonucleotide probe, the second oligonucleotide probe including a moiety at a 3'"'"' end which prevents extension of the second oligonucleotide probe by a polymerase.

30. The kit according to claim 27 where the pairs of oligonucleotide probes include a first oligonucleotide probe and second oligonucleotide probe which hybridizes to the target polynucleotide 3'"'"' relative to the first oligonucleotide probe, the first oligonucleotide probe including a moiety attached to a 5'"'"' end which does not hybridize to the amplification product and modifies the mobility of the ligation product in a size dependent separation process.

31. The kit according to claim 30 wherein the mobility modifier is a polymer chain.

32. The kit according to claim 30 wherein the polymer chain includes polyethylene oxide or polypropylene subunits.

33. The kit according to claim 27 wherein the plurality of target polynucleotides are alleles or mutations of a gene encoding cystic fibrosis transmembrane conductance regulator (CFTR), the isolated amplification primers being capable of hybridizing to contiguous sequences of the CFTR allele or mutation, the isolated oligonucleotide probes being capable of hybridizing to contiguous sequences of the plurality of amplification products.

34. The kit according to claim 33 wherein the allele or mutation of a gene encoding cystic fibrosis transmembrane conductance regulator (CFTR) is selected from the group consisting of Δ

F508, G542X, G551D, W1282X, N1303K 3905insT, 3849+10kbCT, 3849+4AG, 3659delC, R117H, R1162X, 1717-1 GA, 621+1GT, R553X, 2789+5GA, R347P, 2184delA, 1078delT, R334W, 711+1GT, G85E, 1898+1GA, A455E, S549R, S549N, R560T, Δ

l507, Q493X, V520F and Y122X.