Method for determining a level of oxidative stress of a tissue sample
First Claim
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1. A method for determining a level of oxidative stress of a tissue sample, comprising:
- (i) irradiating the sample with electromagnetic radiation at a plurality of wavelengths including a reference wavelength and a sample wavelength, wherein absorption of light at the sample wavelength varies with a state of oxidation of unsaturated fatty acids in the tissue sample, and the reference wavelength is sufficiently removed from the sample wavelength so that absorption of light at the reference wavelength is substantially insensitive to concentration of oxidized unsaturated fatty acids in the tissue sample;
(ii) spectrophotometrically detecting radiation absorbed or scattered by the sample at each of the sample and reference wavelengths, wherein absorbance at the sample wavelength by the tissue sample correlates with the state of oxidation of unsaturated fatty acids in the tissue sample;
(iii) determining a ratio of absorbance at the sample wavelength to absorbance at the reference wavelength for the tissue sample, said ratio being correlated to an oxidative stress level of the tissue;
(iv) ascertaining the oxidative stresses level of the tissue by comparing the ratio determined in (iii) with a predetermined ratio value measured in one or more standard tissue samples of known oxidative stress levels.
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Abstract
Systems and methods for material analysis are disclosed in which an organic sample (e.g., a foodstuff, tissue sample or petroleum product) is illuminated at a plurality of discrete wavelengths which are absorbed by fatty acid and fatty acid oxidation products in the sample. Measurements of the intensity of reflected or absorbed light at such wavelengths are taken, and a analysis of absorbance ratios for various wavelengths is performed. Changes in the reflection ratios are correlated with the oxidative state of fatty acids present in the material.
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7 Claims
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1. A method for determining a level of oxidative stress of a tissue sample, comprising:
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(i) irradiating the sample with electromagnetic radiation at a plurality of wavelengths including a reference wavelength and a sample wavelength, wherein absorption of light at the sample wavelength varies with a state of oxidation of unsaturated fatty acids in the tissue sample, and the reference wavelength is sufficiently removed from the sample wavelength so that absorption of light at the reference wavelength is substantially insensitive to concentration of oxidized unsaturated fatty acids in the tissue sample; (ii) spectrophotometrically detecting radiation absorbed or scattered by the sample at each of the sample and reference wavelengths, wherein absorbance at the sample wavelength by the tissue sample correlates with the state of oxidation of unsaturated fatty acids in the tissue sample; (iii) determining a ratio of absorbance at the sample wavelength to absorbance at the reference wavelength for the tissue sample, said ratio being correlated to an oxidative stress level of the tissue; (iv) ascertaining the oxidative stresses level of the tissue by comparing the ratio determined in (iii) with a predetermined ratio value measured in one or more standard tissue samples of known oxidative stress levels. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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