Materials and methods for the immobilization of bioactive species onto polymeric substrates
First Claim
1. A material having immobilized bioactive species comprising:
- a support member;
a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the Support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-liked together to itself in situ;
a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and
at least one type of bioactive species attached to the second layer.
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Accused Products
Abstract
The present invention is directed to support members having surfaces that are rendered hydrophilic for use as a substrate for the immobilization of bioactive species thereon. The hydrophilic surfaces are chemically stable on the support member. The surfaces are also chemically variable to provide a variety of chemically functional groups for immobilization of bioactive species thereto. The surfaces comprise polymeric surfactants attached onto the surfaces of a support member and covalently cross-linked thereon to form a first layer. Hydrophilic polymers are then attached to the first layer on the support member to form a second layer thereon. The second layer is used to enhance the hydrophilicity of a support member, as well as, to provide a substrate for immobilizing bioactive species thereto. Methods for forming the first and second layers are also provided. In addition methods for attaching bioactive substances to the hydrophilic polymers are provided.
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Citations
89 Claims
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1. A material having immobilized bioactive species comprising:
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a support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the Support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-liked together to itself in situ; a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and at least one type of bioactive species attached to the second layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76)
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18. A material having immobilized bioactive species comprising:
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a support member comprised of a polytetrafluoroetylene material; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and at least one type of bioactive species attached to the second layer. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26)
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27. A material having immobilized bioactive species comprising:
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a hydrophobic support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ; a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and at least one type of bioactive species attached to the second layer. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A material having immobilized bioactive species comprising:
a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-linked to itself in situ; and
at least one type of bioactive species attached to the first layer.- View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47)
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48. A material having immobilized bioactive species comprising:
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a support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-linked to itself in situ; a plurality of layers attached to one another wherein each layer is comprised of at least one species of a hydrophilic polymer and wherein at least one layer of the plurality of layers is covalently attached to the first layer; and at least one type of bioactive species attached to at least one layer of the plurality of layers. - View Dependent Claims (49, 50, 51, 52, 53, 54, 55, 56)
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57. A method of making a material having immobilized bioactive species which comprises:
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providing a hydrophobic support member; attaching a first layer comprised of polymeric surfactants to the support member non-covalently; cross-linking the polymeric surfactants in situ with a cross-linking agent; attaching a second layer comprised of at least one type of hydrophilic polymer to the first layer convalently; and attaching a bioactive species to the second layer. - View Dependent Claims (58, 59, 60, 61, 62, 63, 64, 65, 66)
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77. A material having immobilized bioactive species comprising:
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a hydrophobic support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ; and at least one type of bioactive species attached to the first layer. - View Dependent Claims (78, 79, 80, 81, 82)
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83. A material having immobilized bioactive species comprising:
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a hydrophobic support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ; a plurality of layers attached to one another wherein each layer is comprised of at least one species of a hydrophilic polymer and wherein at least one layer of the plurality of layers is covalently attached to the first layer; and at least one type of bioactive species attached to at least one layer of the plurality of layers. - View Dependent Claims (84, 85, 86, 87, 88, 89)
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Specification