Keratin K1 expression vectors and methods of use
First Claim
1. A vector for expression of a nucleic acid sequence in an epidermal cell, wherein said nucleic acid sequence is other than a mammalian K1 keratin gene, said vector comprising, in operable association:
- a 5'"'"' flanking region from a mammalian gene including necessary sequences for expression of said nucleic acid sequence;
a 3'"'"' flanking region from a mammalian K1 keratin gene which regulates expression predominantly in an epidermal cell of said nucleic acid sequence with which it is associated; and
a linker connecting said 5'"'"' flanking region to said nucleic acid sequence, said linker having a position for inserting said nucleic acid sequence, wherein said linker lacks the coding sequences of a gene with which it is naturally associated.
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Abstract
A keratin K1 vector for expression of a nucleic acid sequence in an epidermal cell. The vector includes a 5'"'"' flanking region which includes necessary sequences for expression of a nucleic acid cassette, a keratin K1 3'"'"' flanking region which regulates expression of a nucleic acid sequence, predominantly in the epidermis, and a linker which connects the 5'"'"' flanking region to a nucleic acid. The linker has a position for inserting a nucleic acid cassette. The linker does not contain the coding sequence of a gene that the linker is naturally associated with. That is, the linker is not the normal gene associated with the 5'"'"' and 3'"'"' regions.
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Citations
34 Claims
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1. A vector for expression of a nucleic acid sequence in an epidermal cell, wherein said nucleic acid sequence is other than a mammalian K1 keratin gene, said vector comprising, in operable association:
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a 5'"'"' flanking region from a mammalian gene including necessary sequences for expression of said nucleic acid sequence; a 3'"'"' flanking region from a mammalian K1 keratin gene which regulates expression predominantly in an epidermal cell of said nucleic acid sequence with which it is associated; and a linker connecting said 5'"'"' flanking region to said nucleic acid sequence, said linker having a position for inserting said nucleic acid sequence, wherein said linker lacks the coding sequences of a gene with which it is naturally associated. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. An isolated or cultured mammalian epidermal cell transformed with a vector for expression of a nucleic acid sequence, wherein said nucleic acid sequence is other than a mammalian K1 keratin gene, said vector comprising, in operable association:
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a 5'"'"' flanking region from a mammalian gene including necessary sequences for expression of said nucleic acid sequence; a 3'"'"' flanking region from a mammalian K1 keratin gene which regulates expression predominantly in an epidermal cell of said nucleic acid sequence with which it is associated; and a linker connecting said 5'"'"' flanking region to said nucleic acid sequence, said linker having a position for inserting said nucleic acid sequence, wherein said linker lacks the coding sequences of a gene with which it is naturally associated. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification