Method and arrangement for monitoring a separating liquid stream

• US 5,916,449 A
• Filed: 12/03/1996
• Issued: 06/29/1999
• Est. Priority Date: 12/28/1995
• Status: Expired due to Fees

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Ellwart, Joachim, Karls, Ingolf
• Primary Examiner(s): Drodge, Joseph W.
• Application Number:
  US08/754,036
• Time in Patent Office: 938 Days
• Field of Search: 210/94, 210/745, 422/82.05, 422/82.08, 422/73, 422/105, 436/63, 436/164, 436/172, 356/39, 356/72, 356/73, 382/128, 382/129, 382/133, 382/134, 382/173, 382/180, 209/3.1, 209/3.2, 209/3.3, 209/579, 209/906, 209/576, 209/577
• US Class Current: 210/745
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1406 Control of droplet point
• CPC Class Codes: Y10S 209/906 Pneumatic or liquid stream feeding item

Abstract

A method and an arrangement by which separating liquid streams, such as occur for example in cell sorters, are monitored automatically. An image of the separating liquid streams is recorded and the position of a separation point at which the drops separate from a column of liquid is found using image analysis methods. Various solution possibilities are indicated for the suitable preparation of the image information for easier processing.

Claims

1. An arrangement for monitoring a separating liquid stream, comprising:

a detection device operable for detection of a variable position of a separation point at which individual drops separate from a column of liquid by detecting a pixel image;

an evaluation device operable for evaluation of the detected position of the separation point with respect to at least one predetermined position boundary value by row or column analysis of the image, the evaluation device comparing the detected position with the at least one position boundary value; and

an acting device that is set into action upon the detected position deviating more than a predetermined distance from a predetermined reference position as determined by the evaluation device.

2. The arrangement according to claim 1, wherein the detection device is a monitoring camera that is connected to and that operates together with image storage devices for storing at least one momentary photograph of the separation point.

3. The arrangement according to claim 1, wherein the evaluation device is a computer.

4. The arrangement according to claim 3, wherein the computer has a graphics card with a frame grabber, and wherein the computer is structured such that a graphic evaluation of each of the at least one position boundary value is based on pixel information stored as a momentary image, the position boundary value being predetermined in the form of pixel coordinate values.

5. The arrangement according to claim 1, wherein the acting device is an alarm device.

6. The arrangement according to claim 1, wherein the acting device automatically interrupts the liquid stream.

7. The arrangement according to claim 1, wherein the acting device is an actuator that readjusts the separation point.

8. The arrangement according to claim 1, wherein the separation point of the liquid stream in a cell sorter is monitored.

9. A method for monitoring a separating liquid stream, comprising the steps of:

acquiring a pixel image of a separation point at which individual drops separate from a column of liquid;

determining a position of the separation point in the image by row or column analysis of the image, thereof;

comparing the determined position of the separation point to at least one boundary value for the position; and

introducing a measure dependent on the determined position deviating more than a predetermined distance from a predetermined reference point based on said comparison.

10. The method according to claim 9, wherein the method further comprises:

determining drop direction for the image analysis by examining pixel information of the image row by row or, alternatively, column by column for locations of higher coloration, and connecting these locations by a straight line after averaging, which line forms a line of symmetry of a drop stream that is formed by the individual drops; and

determining a location of lower coloration along the line of symmetry, beginning at a known side of entry of the column of liquid into the image and continuing to the other side of the image, said location of lower coloration being a position of the separation point in the image.

11. The method according to claim 10, wherein an extreme value of color values of the pixels is determined in a row and/or column direction, and wherein only two discrete values are allocated to the color values of the pixels, used for undershooting thereof in allocation of extreme values, so that all values that exceed the extreme value receive one color value, and all other values in the respective row and/or column receive the other color value.

12. The method according to claim 9, wherein a search window is defined around the separation edge, said window being substantially smaller than the image.

13. The method according to claim 9, wherein the measure is an interruption of the liquid stream.

14. The method according to claim 9, wherein the measure is the removing of a small sorting tube from a stream of the individual droplets.

15. An arrangement for monitoring a separating liquid stream, comprising:

a detection device operable for detection of a variable position of a separation point at which individual drops separate from a column of liquid, the detection device being a monitoring camera that is connected to and that operates together with image storage devices for storing at least one momentary photograph of the separation point;

a computer operable for evaluation of the detected position of the separation point in dependence on at least one predetermined position boundary value, the computer comparing the detected position with the at least one position boundary value, the computer being structured such that a graphic evaluation of the position boundary value is based on row or column analysis of pixel information stored as a momentary image, the position boundary value being predetermined in the form of pixel coordinate values;

an alarm device that is activated by a result of the comparing that indicates the detected position deviating more than a predetermined distance from a predetermined reference position as determined by the computer.

16. The arrangement according to claim 15, wherein said result of the comparison effects automatic interruption of the liquid stream.

17. The arrangement according to claim 15, wherein said result of the comparison activates an actuator that readjusts the separation point.

18. The arrangement according to claim 15, wherein the separation point of the liquid stream in a cell sorter is monitored.