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Continuous process for the separation of biologic components from heterogeneous cell populations

  • US 5,916,743 A
  • Filed: 06/06/1995
  • Issued: 06/29/1999
  • Est. Priority Date: 09/13/1990
  • Status: Expired due to Term
First Claim
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1. A method for separating a specific cell population from a cell suspension comprising:

  • establishing fluid communication between the vascular system of a human and a rotating cell processing device;

    withdrawing a cell suspension that includes a specific cell population from the human;

    providing particles comprising a binding site capable of selectively binding to said specific cell population of said suspension;

    continuously introducing at least a portion of said cell suspension including cells of said specific cell population into said rotating cell processing device;

    continuously feeding said particles into said rotating cell processing device;

    forming particle/cell conjugates from said suspension and particles in said rotating cell processing device; and

    separating said particle/cell conjugates from said remainder of said cell suspension within said rotating cell processing device during said introducing and said feeding steps.

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