Continuous process for the separation of biologic components from heterogeneous cell populations
First Claim
1. A method for separating a specific cell population from a cell suspension comprising:
- establishing fluid communication between the vascular system of a human and a rotating cell processing device;
withdrawing a cell suspension that includes a specific cell population from the human;
providing particles comprising a binding site capable of selectively binding to said specific cell population of said suspension;
continuously introducing at least a portion of said cell suspension including cells of said specific cell population into said rotating cell processing device;
continuously feeding said particles into said rotating cell processing device;
forming particle/cell conjugates from said suspension and particles in said rotating cell processing device; and
separating said particle/cell conjugates from said remainder of said cell suspension within said rotating cell processing device during said introducing and said feeding steps.
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Abstract
A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.
165 Citations
61 Claims
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1. A method for separating a specific cell population from a cell suspension comprising:
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establishing fluid communication between the vascular system of a human and a rotating cell processing device; withdrawing a cell suspension that includes a specific cell population from the human; providing particles comprising a binding site capable of selectively binding to said specific cell population of said suspension; continuously introducing at least a portion of said cell suspension including cells of said specific cell population into said rotating cell processing device; continuously feeding said particles into said rotating cell processing device; forming particle/cell conjugates from said suspension and particles in said rotating cell processing device; and separating said particle/cell conjugates from said remainder of said cell suspension within said rotating cell processing device during said introducing and said feeding steps. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 42, 43, 46, 47, 49, 50, 51, 53, 54, 56, 57, 58)
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21. A method for separating a specific cell population from a cell suspension comprising:
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establishing fluid communication between the vascular system of a human and a rotating cell processing device; withdrawing a cell suspension that includes a specific cell population from a human; obtaining a cell concentrate from said cell suspension, said cell concentrate including said specific cell population; providing particles comprising a binding site capable of selectively binding to said specific cell population of said concentrate; continuously introducing at least a portion of said cell concentrate including cells of said specific cell population into said rotating cell processing device; continuously feeding said particles into said rotating cell processing device; forming particle/cell conjugates from said concentrate and particles in said rotating cell processing device; and separating said particle/cell conjugates from said remainder of said concentrate within said rotating cell processing device during said introducing and said feeding steps. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 48, 52, 55, 59, 60, 61)
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Specification