Multifunctional linking reagents for synthesis of branched oligomers

• US 5,916,750 A
• Filed: 01/08/1997
• Issued: 06/29/1999
• Est. Priority Date: 01/08/1997
• Status: Expired due to Term

• Current Assignee: Biogenex Laboratories Incorporated
• Original Assignee: Biogenex Laboratories Incorporated
• Inventors: Inamdar, Anita, Su, Sheng-Hui, Kalra, Krishan L., Iyer, Rajkumar Siva
• Primary Examiner(s): Houtteman, Scott W.
• Application Number:
  US08/780,725
• Time in Patent Office: 902 Days
• Field of Search: 435/6, 544/83
• US Class Current: 435/6.12
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Reagents capable of forming branched oligomers with monomeric units are disclosed, together with oligomers incorporating such reagents, kits containing such reagents and methods for use of such reagents in forming oligomers with monomeric units. The present reagents can advantageously be used to introduce multiple labels or reporter molecules onto oligomers such as oligonucleotides and oligopeptides. In particular, non-nucleosidic phosphoramidites based on 1,3,5-tris(2-hydroxyethyl)cyanuric acid are disclosed. Multiply-labeled, branched DNA oligomer probes constructed using these phosphoramidite reagents showed increased signal intensity relative to singly-labeled oligomer probes.

Claims

1. A reagent which is capable of forming a oligomer with monomeric units, said reagent comprising a compound of the formula:

##STR12## wherein Each of R¹, R² and R³ is independently selected from the group consisting of hydrogen, blocking groups, capping groups, labels, and groups of the formula;

##STR13## wherein X⁴ is halogen or substituted amino,

X⁵ is alkyl, alkoxy, aryloxy, or a cyano derivative thereof,

X⁶ is halogen, amino or O, and

X⁷ is alkyl, alkoxy or aryloxy, or may be H only if X⁶ is O, with the proviso that at least one of R¹, R² and R³ is not hydrogen;

Each of X¹, X², and X³ is independently selected from the group consisting of compounds of the formula

space="preserve" listing-type="equation">--X.sub.r --Y.sup.4 --

where r is an integer at least 1, each X is independently C or O, and each C can independently be substituted or unsubstituted, saturated or unsaturated, and Y⁴ is selected from the group consisting of O, S, NH, N═

N and a direct bond linking X_r with R¹, R², or R³ ; and

Each of Y¹, Y², and Y³ is independently O or S.

2. A reagent as recited in claim 1, wherein R¹ is selected from the group consisting of acid-labile, base-stable blocking groups.

3. A reagent as recited in claim 2, wherein R¹ is dimethoxytrityl.

4. A reagent as recited in claim 1, wherein R² is selected from the group consisting of acid-stable, base-labile blocking groups.

5. A reagent as recited in claim 4, wherein R² is a levulinate ester.

6. A reagent as recited in claim 1, wherein each of X¹, X₂, and X³ is --(CH₂)₂ --Y⁴.

7. A reagent as recited in claim 6, wherein Y⁴ is O.

8. A reagent as recited in claim 1, wherein each of Y¹, Y², and Y³ is O.

9. A reagent as recited in claim 1, wherein R³ is selected from the group consisting of phosphodiesters, phosphotriesters, phosphites, phosphoramidites, H-phosphonates, alkyl-phosphonates, and phosphorothioates.

10. A reagent as recited in claim 1, wherein R³ comprises a bond, either directly or through an intermediate group, to a solid support.

11. A reagent as recited in claim 1, wherein

R¹ is dimethoxytrityl,

R² is dimethoxytrityl,

R³ is β

-cyanoethyl-N,N-diisopropyl phosphoramidite, each of X¹, X², and X³ is --(CH₂)₂ --Y⁴, where

Y⁴ is O for X¹ and X² and is a direct bond linking X_r with R³ for X³, and each of Y¹, Y², and Y³ is O.

12. A reagent as recited in claim 1, wherein

R¹ is dimethoxytrityl,

R² is a levulinate ester,

R³ is β

-cyanoethyl-N,N-diisopropyl phosphoramidite, each of X¹, X², and X³ is --(CH₂)₂ --Y⁴, where

Y⁴ is O for X¹ and X² and is a direct bond linking X_r with R³ for X³, and each of Y¹, Y₂, and Y³ is O.

13. A reagent as recited in claim 1, wherein

R¹ is dimethoxytrityl,

R² is a functional moiety attached through a linker arm,

R³ is phosphoramidite, H-phosphonate or a bond to LCAA-CPG, each of X¹, X², and X³ is --(CH₂)₂ --Y⁴,

Y⁴ is O, and each of Y¹, Y₂, and Y³ is O.

14. A reagent as recited in claim 1, wherein at least one of R¹, R² and R³ comprises a functional moiety selected from the group consisting of detectable labels, intercalating agents, metal chelators, drugs, hornones, proteins, peptides, free radical generators, nucleolytic agents, proteolytic agents, catalysts, specific binding agents, agents which modify DNA transport across a biological barrier, and substances which alter the solubility of a nucleotide oligomer.

15. A dimeric reagent comprising two reagents as recited in claim 1.

16. A dimeric reagent as recited in claim 15, wherein:

each R¹ and R² is dimethoxytrityl or levulinic acid ester,

each R³ is attached either directly or by a linking group to a phosphoramidite, H-phosphonate or LCAA-CPG,

each of X¹, X², and X³ is --(CH₂)₂ --Y⁴,

Y⁴ is O, and each of Y¹, Y², and Y³ is O.

17. A nucleic acid oligomer having nucleotide and non-nucleotide monomeric units, at least one of said non-nucleotide units comprising a compound of the formula:

##STR14## wherein Each of R¹, R² and R³ is independently selected from the group consisting of hydrogen, blocking groups, capping groups, labels, linking groups, bonds to adjacent units and groups of the formula ##STR15## wherein X⁴ is halogen or substituted amino,

X⁵ is alkyl, alkoxy, aryloxy, or a cyano derivative thereof,

X⁶ is halogen, amino or O, and

X⁷ is alkyl, alkoxy or aryloxy, or may be H only if X⁶ is O, with the proviso that at least one of R¹, R² and R³ is not hydrogen;

Each of X¹, X₂, and X³ is independently selected from the group consisting of compounds of the formula

space="preserve" listing-type="equation">--X.sub.r --Y.sup.4 --

where r is an integer at least 1, each X is independently C or O, and each C can independently be substituted or unsubstituted, saturated or unsaturated, and Y⁴ is selected from the group consisting of, O, S, NH, N═

N and a direct bond linking X_r with R¹, R², or R³ ; and

Each of Y¹, Y², and Y³ is independently O or S, said non-nucleotide unit bound to at least one nucleotide monomeric unit by a bond or linking group at either R¹, R² or R³.

18. A method for preparing a nucleic acid oligomer having both nucleotide and non-nucleotide units, comprising

coupling at least one unit comprising a compound of the formula:

##STR16## wherein Each of R¹, R² and R³ is independently selected from the group consisting of hydrogen, blocking groups, capping groups, labels, linking groups, bonds to adjacent units, and groups of the formula ##STR17## wherein X⁴ is halogen or substituted amino,

X⁵ is alkyl, alkoxy, aryloxy, or a cyano derivative thereof,

X⁶ is halogen, amino or O, and

X⁷ is alkyl, alkoxy or aryloxy, or may be H only if X⁶ is O, with the proviso that at least one of R¹, R² and R³ is not hydrogen;

Each of X¹, X₂, and X³ is independently selected from the group consisting of compounds of the formula

space="preserve" listing-type="equation">--X.sub.r --Y.sup.4 --

where r is an integer at least 1, each X is independently C or O, and each C can independently be substituted or unsubstituted, saturated or unsaturated, and Y⁴ is selected from the group consisting of, O, S, NH N═

N and a direct bond linking X_r with R¹, R², or R³ ; and

Each of Y¹, Y², and Y³ is independently O or S, to at least one nucleotide unit by a bond or linking group at either R¹, R² or R³.

19. A method as recited in claim 18 further comprising attaching at least one detectable label to at least one of R¹, R² and R³.

20. A kit for preparing a nucleic acid oligomer having both nucleotide and non-nucleotide units, comprising

a receptacle adapted to hold one or more individual reagent containers;

and

a first container containing a reagent in accordance with the formula;

##STR18## wherein Each of R¹, R² and R³ is independently selected from the group consisting of hydrogen, blocking groups, capping groups, labels, and groups of the formula ##STR19## wherein X⁴ is halogen or substituted amino,

X⁵ is alkyl, alkoxy, aryloxy, or a cyano derivative thereof,

X⁶ is halogen, amino or O, and

X⁷ is alkyl, alkoxy or aryloxy, or may be H only if X⁶ is O, with the proviso that at least one of R¹, R² and R³ is not hydrogen;

Each of X¹, X², and X³ is independently selected from the group consisting of compounds of the formula

space="preserve" listing-type="equation">--X.sub.r --Y.sup.4 --

where r is an integer at least 1, each X is independently C or O, and each C can independently be substituted or unsubstituted, saturated or unsaturated, and Y⁴ is selected from the group consisting of, O, S, NH, N═

N and a direct bond linking X_r with R¹, R², or R³ ; and

Each of Y¹, Y², and Y³ is independently selected from the group consisting of O and S.

21. A kit as recited in claim 20, further comprising a second container containing (1) a reagent used in the synthesis of oligomers, or (2) a reagent used in the detection of a functional moiety associated with said reagent.

22. A kit as recited in claim 20, further comprising a second container containing a reagent used in connection with a functional moiety selected from the group consisting of detectable labels, intercalating agents, metal chelators, drugs, hormones, proteins, peptides, free radical generators, nucleolytic agents, proteolytic agents, catalysts, specific binding agents, agents which modify DNA transport across a biological barrier, and substances which alter the solubility of a nucleotide oligomer.

23. A method for detecting the presence or amount of an analyte in a sample comprising

providing a sample suspected of containing an analyte of interest;

providing a nucleic acid oligomer having both nucleotide and non-nucleotide units, at least one of said non-nucleotide units comprising a compound of the formula;

##STR20## wherein Each of R¹, R² and R³ is independently selected from the group consisting of hydrogen, blocking groups, capping groups, labels, linking groups, bonds to adjacent units, and groups of the formula ##STR21## wherein X⁴ is halogen or substituted amino,

X⁵ is alkyl, alkoxy, aryloxy, or a cyano derivative thereof,

X⁶ is halogen, amino or O, and

X⁷ is alkyl, alkoxy or aryloxy, or may be H only if X⁶ is O, with the proviso that at least one of R¹, R² and R³ further comprises a detectable label,

Each of X¹, X², and X³ is independently selected from the group consisting of compounds of the formula

space="preserve" listing-type="equation">--X.sub.r --Y.sup.4 --

where r is an integer at least 1, each X is independently C or O, and each C can independently be substituted or unsubstituted, saturated or unsaturated, and Y⁴ is selected from the group consisting of, O, S, NH, N═

N and a direct bond linking X_r with R¹, R², or R³ ; and

Each of Y¹, Y², and Y³ is independently O or S, said non-nucleotide unit bound to at least one nucleotide monomeric unit by a bond or a linking group at either R¹, R² or R³, and wherein said oligomer is configured so as to be able to hybridize with said analyte;

exposing said sample to said oligomer for a time and under conditions which permit said oligomer to hybridize with any analyte present in said sample; and

detecting the presence or amount of said analyte in said sample.

24. A method as recited in claim 23, wherein said oligomer is bound to a solid support selected from the group consisting of glass beads, microbeads, resins, polystyrene, membranes, and microtiter plates.

25. A method as recited in claim 24, wherein said solid support comprises controlled pore glass.

26. A method as recited in claim 24, wherein said solid support comprises a microtiter plate.