Attachment of unmodified nucleic acids to silanized solid phase surfaces
First Claim
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1. A method for immobilizing an unmodified nucleic acid molecule to a glass or plastic solid phase, which comprises the steps of:
- (A) coating said solid phase with a mercapto-silane or an epoxy-silane and allowing said silane-coated solid phase to cure; and
(B) coupling said unmodified nucleic acid molecule having either a terminal 3'"'"' OH or a terminal 5'"'"' OH to said silane-coated solid phase.
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Abstract
The invention relates to a simple, cost effective method for immobilizing synthetic nucleic acid molecules onto a solid support. The invention further concerns the use of such immobilized molecules in nucleic acid hybridization assays, sequencing by hybridization assays, and genetic analyses and combinatorial analyses involving nucleic acids or proteins for screening applications.
239 Citations
29 Claims
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1. A method for immobilizing an unmodified nucleic acid molecule to a glass or plastic solid phase, which comprises the steps of:
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(A) coating said solid phase with a mercapto-silane or an epoxy-silane and allowing said silane-coated solid phase to cure; and (B) coupling said unmodified nucleic acid molecule having either a terminal 3'"'"' OH or a terminal 5'"'"' OH to said silane-coated solid phase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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- 28. A method for immobilizing an unmodified nucleic acid molecule to a mercapto-silane or epoxy-silane-coated solid phase comprising coupling said unmodified nucleic acid molecule, wherein said nucleic acid molecule has either a terminal 3'"'"' OH or a terminal 5'"'"' OH, to said silane-coated solid phase.
Specification