Production of cartilage tissue using cells isolated from Wharton's jelly
First Claim
1. A method of isolating a pre-chondrocyte from Wharton'"'"'s jelly of the umbilical cord comprising, collecting Wharton'"'"'s jelly comprising pre-chondrocytes from an umbilical cord, culturing the Wharton'"'"'s jelly in vitro under conditions wherein said pre-chondrocytes proliferate, and isolating the pre-chondrocyte therefrom.
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Abstract
The invention relates to the isolation and use of pre-chondrocytes from the umbilical cord, specifically from Wharton'"'"'s jelly, that give rise to chondrocytes which produce cartilage. The isolated pre-chondrocytes, or the chondrocytes to which they give rise, can be mitotically expanded in culture and used in the production of new cartilage tissue for therapeutic use. "Banks" of pre-chondrocytes or chondrocytes can be stored frozen, and thawed and used to produce new cartilage tissue as needed.
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Citations
6 Claims
- 1. A method of isolating a pre-chondrocyte from Wharton'"'"'s jelly of the umbilical cord comprising, collecting Wharton'"'"'s jelly comprising pre-chondrocytes from an umbilical cord, culturing the Wharton'"'"'s jelly in vitro under conditions wherein said pre-chondrocytes proliferate, and isolating the pre-chondrocyte therefrom.
- 3. A method of isolating a chondrocyte from Wharton'"'"'s jelly of the umbilical cord comprising, collecting Wharton'"'"'s jelly comprising pre-chondrocytes from an umbilical cord, culturing the Wharton'"'"'s jelly in vitro under conditions wherein said pre-chondrocytes proliferate, isolating the pre-chondrocytes therefrom, culturing the pre-chondrocytes under conditions that induce the production of chondrocytes capable of producing cartilage tissue, and isolating the chondrocyte.
Specification