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Assay for glycated blood proteins

  • US 5,919,708 A
  • Filed: 12/11/1995
  • Issued: 07/06/1999
  • Est. Priority Date: 11/14/1990
  • Status: Expired due to Fees
First Claim
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1. A method of assessing a glycated blood protein in a sample, said method comprising the steps of:

  • a) optionally hemolyzing said sample to liberate cell bound glycated blood protein;

    b) separating said glycated blood protein and the corresponding non-glycated blood protein from said sample using a liquid phase precipitation agent, or means substantially specific for the protein being assessed;

    c) contacting said non-immobilized separated proteins with a first signal forming agent capable of binding to said glycated protein with substantially higher binding affinity than for the corresponding non-glycated protein;

    d) optionally, contacting said non-immobilized separated proteins with a second signal forming agent capable of binding to said glycated protein and to said corresponding non-glycated protein; and

    e) assessing the signal forming agents which have bound to said non-immobilized separated proteins or which have not bound to said glycated protein or said corresponding non-glycated protein;

    with the proviso that where said glycated protein comprises glycated hemoglobin said first signal forming agent is a chromophore labelled boronic acid or salt thereof having an absorption maximum at above 600 nm.

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