Biosensor and method for quantitating biochemical substrate using the same
First Claim
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1. A biosensor comprising:
- an insulating substrate;
an electrode system formed on the insulating substrate which comprises a working electrode and a counter electrode; and
a reaction layer formed on the insulating substrate which contains an oxidoreductase and a ferrocene electrolyte as an electron acceptor,wherein the ferrocene electrolyte consists of a ferricinium ion and a mono-anion and the ferrocene electrolyte is selected from the group consisting of ferricinium hexafluorophosphate and ferricinium tetrafluoroborate.
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Abstract
A biosensor includes: an insulating substrate; an electrode system formed on the insulating substrate which has a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate which contains an oxidoreductase and an electron acceptor. The electron acceptor is ferricinium ion derived from ferrocene electrolyte.
169 Citations
9 Claims
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1. A biosensor comprising:
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an insulating substrate; an electrode system formed on the insulating substrate which comprises a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate which contains an oxidoreductase and a ferrocene electrolyte as an electron acceptor, wherein the ferrocene electrolyte consists of a ferricinium ion and a mono-anion and the ferrocene electrolyte is selected from the group consisting of ferricinium hexafluorophosphate and ferricinium tetrafluoroborate. - View Dependent Claims (2, 3, 4)
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5. A method for quantitating the concentration of a biochemical substrate in a sample liquid by using a biosensor comprising an insulating substrate, an electrode system formed on the insulating substrate which has a working electrode and a counter electrode and a reaction layer provided on the insulating substrate which contains an oxidoreductase and a ferrocene electrolyte as an electron acceptor, comprising the steps of:
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adding the sample liquid to the reaction layer; and detecting a response current value by applying a voltage between the working electrode and the counter electrode, wherein the ferrocene electrolyte consists of a ferricinium ion and a mono-anion and the ferrocene electrolyte is selected from the group consisting of ferricinium hexafluorophosphate and ferricinium tetrafluoroborate. - View Dependent Claims (6, 7, 8, 9)
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Specification