Integrated fluorescence-based biochemical sensor

• US 5,922,285 A
• Filed: 10/01/1997
• Issued: 07/13/1999
• Est. Priority Date: 10/01/1996
• Status: Expired due to Term

• Current Assignee: Texas Instruments, Inc.
• Original Assignee: Texas Instruments, Inc.
• Inventors: Melendez, Jose, Arbuthnot, Diane L., Carr, Richard A.
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US08/942,090
• Time in Patent Office: 650 Days
• Field of Search: 422/82.05, 422/82.11, 422/82.08, 422/91, 436/165, 436/172
• US Class Current: 422/82.08
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 2201/022 Casings

Abstract

An integrated biochemical sensor (200) for detecting the presence of one or more specific samples (240) having a device platform (355) with a light absorbing upper surface and input/output pins (375) is disclosed. An encapsulating housing (357) provides an optical transmissive enclosure which covers the platform (355) and has a layer of fluorescence chemistry on its outer surface (360). The fluorophore is chosen for its molecular properties in the presence of the sample analyte (240). The detector (370), light sources (365, 367, 407, 409) are all coupled to the platform (355) and encapsulated within the housing (357). A filter (375) element is used to block out unwanted light and increase the detector'"'"'s (370) ability to resolve wanted emission light.

Claims

1. A sensor assembly comprising:

a platform having an upper surface and a bottom surface;

a light source coupled to said upper surface of said platform;

a detector coupled to said upper surface of said platform adjacent said light source; and

an encapsulating housing extending over said platform and arranged to receive light from said light source and reflect a portion of said light in a direction away from said detector;

wherein said housing has an outer surface, and a portion of said outer surface of said housing comprises a sensing surface;

and wherein the geometric configuration of said housing is such that light from said light source which is reflected off said sensing surface is diverted away from said detector.

2. The sensor assembly according to claim 1 further including an absorbent filter spatially arranged about said detector to minimize light incident upon said detector from said light source.

3. The sensor according to claim 1 wherein said detector comprises a solid-state photodiode.

4. The sensor according to claim 1 wherein said light source is a light emitting diode that emits light that is substantially blue.

5. The sensor according to claim 1 further comprising an interface coupled to said bottom of said platform.

6. The sensor according to claim 1 further comprising a fluorescence emitting chemistry layer predisposed over said sensing surface and having a characteristic quality that interacts with a specific analyte sample to produce a change in the fluorescence emission.

7. The sensor according to claim 6 wherein said light source is a light emitting diode that emits light having an excitation wavelength that interacts with the fluorescence emitting chemistry layer.

8. The sensor according to claim 6 wherein said fluorescence emitting chemistry layer is deposited on the sensing surface by spin coating.

9. The sensor according to claim 6 further comprising a filter interposed between said light source and said fluorescence emitting chemistry layer for blocking light not within the excitation wavelength of the fluorescence emitting chemistry layer.

10. The sensor according to claim 1 further including an optical sink spatially arranged to intercept stray light inside said housing.

11. The sensor according to claim 10 wherein said optical sink is a light absorbing material which coats the upper surface of said platform.

12. The sensor according to claim 10 wherein said a optical sink is an optical sub-assembly which directs light incident upon it outside said housing.