Integrated nucleic acid diagnostic device
First Claim
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1. A miniature fluidic system, comprising:
- a body having at least two discrete reaction chambers, each of said reaction chambers comprising at least one vent port, and wherein each of said reaction chambers is fluidly connected to a common chamber or channel by a fluidic inlet having a controllable diaphragm valve disposed thereacross; and
a pneumatic system for selectively applying a pressure differential between said common channel or chamber and at least a selected one of said at least two discrete chambers, whereby said pressure differential directs a fluid sample in said body between said common channel or chamber and said at least one selected chamber.
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Abstract
The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved in sample acquisition and storage, sample preparation and sample analysis, within a single integrated unit. The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo sequencing applications.
1242 Citations
58 Claims
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1. A miniature fluidic system, comprising:
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a body having at least two discrete reaction chambers, each of said reaction chambers comprising at least one vent port, and wherein each of said reaction chambers is fluidly connected to a common chamber or channel by a fluidic inlet having a controllable diaphragm valve disposed thereacross; and a pneumatic system for selectively applying a pressure differential between said common channel or chamber and at least a selected one of said at least two discrete chambers, whereby said pressure differential directs a fluid sample in said body between said common channel or chamber and said at least one selected chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51)
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52. A miniature fluidic system, comprising:
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a body having at least first and second chambers disposed therein, at least one of said first and second chambers having a vent port with a gas/liquid separator disposed thereacross, each of said at least first and second chambers having a fluid inlet with a controllable diaphragm valve disposed thereacross, said at least first and second chambers being in fluid connection, and at least one of said at least first and second chamber being a hybridization chamber for analyzing a component of a fluid sample, said hybridization chamber including a polymer array, said polymer array including a plurality of different polymer sequences coupled to a surface of a single substrate, each of said plurality of different polymer sequences being coupled to said surface in a different, known location; a sample inlet, fluidly connected to at least one of said first and second chambers, for introducing a fluid sample into said system; a fluid transport system for moving a fluid sample from said at least first chamber to said at least second chamber. - View Dependent Claims (53, 54, 55, 56, 57, 58)
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Specification