Method of producing bilayer lipid membranes

• US 5,922,594 A
• Filed: 03/25/1997
• Issued: 07/13/1999
• Est. Priority Date: 09/26/1994
• Status: Expired due to Term

• Current Assignee: GE Healthcare Bio-Sciences AB (GE Healthcare Technologies, Inc.)
• Original Assignee: Biacore AB (General Electric Company)
• Inventors: Lof.ang.s, Stefan
• Primary Examiner(s): Tsang, Cecilia J.
• Assistant Examiner(s): Lukton, David
• Application Number:
  US08/809,617
• Time in Patent Office: 840 Days
• Field of Search: 435/291, 436/13, 422/57, 264/4.1, 424/420, 424/450, 427/2.14
• US Class Current: 435/287.1
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2610/00 Assays involving self-assembled monolayers [SAMs]
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: Y10S 977/788 Of specified organic or carbon-based composition
• CPC Class Codes: Y10T 436/104165 Lipid, cholesterol, or triglyceride standard or control

Abstract

Methods are disclosed for producing a substrate surface supporting a continuous planar bilayer lipid membrane by covalently binding a plurality of micellar of vesicle liposomes, optionally comprising a membrane protein or other biologically active membrane-bound component, to a substrate surface supporting a self-assembled monolayer (SAM) of essentially straight long chain molecules. In one embodiment, the micellar or vesicle liposomes covantly bind to hydrophilic spacer molecules attached to the functional groups of the self-assembled monolayer.

Claims

1. A method of producing a substrate surface supporting a bilayer lipid membrane, comprising the steps of:

providing a substrate, wherein the substrate has coated thereon a self-assembled monolayer of essentially straight long chain molecules having functional groups;

contacting the substrate with an aqueous solution comprising micellar of vesicle liposomes such that a plurality of the micellar or vesicle liposomes covalently bond to the functional groups of the self-assembled monolayer so as to form the substrate surface supporting the bilayer lipid membrane.

2. The method according to claim 1, wherein the micellar or vesicle liposomes covalently bond to hydrophilic spacer molecules, wherein the hydrophilic spacer molecules are attached to the functional groups of the self-assembled monolayer.

3. The method according to claim 1, wherein the micellar or vesicle liposomes comprise a membrane protein.

4. The method according to claim 1 or 2, wherein the straight long chain molecules are derived from hydrocarbons.

5. The method according to claim 4, wherein the straight long molecules comprise alkane thiols.

6. The method according to claim 5, wherein the alkane thiols have at least ten atoms in their chain.

7. The method according to claim 1, wherein the straight long chain molecules are selected to provide a hydrophilic surface.

8. The method according to claim 7, wherein the straight long chain molecules comprise hydroxyalkane thiols.

9. The method according to claim 1, wherein the straight long chain molecules comprise a mixture of molecules with different functionalities.

10. The method according to claim 1, wherein the straight long chain molecules are bounded to a metal.

11. The method according to claim 10, wherein the metal is gold.

12. The method according to claim 1, wherein the step of contacting the substrate with the aqueous solution comprising micellar or vesicle liposomes is carried out in a flow cell using a controlled laminar flow.

13. The method according to claim 1, wherein the method further comprisis monitoring the formation of the bilayer lipid membrane by a surface technique that detects measurable changes in a property associated with the substrate.

14. The method according to claim 13, wherein the method further comprises monitoring molecular interactions between an analyte and the bilayer lipid membrane by the surface sensing technique.

15. The method according to claim 13 or 14, wherein the surface sensing technique is based on mass sensing.

16. The method according to claim 13 or 14 wherein the surface sensing technique is based on evanescent wave sensing.

17. The method according to claim 13 or 14 wherein the surface sensing technique is based on surface plasmon resonance.