Surface plasmon sensor having a coupler with a refractive index matching liquid

• US 5,923,031 A
• Filed: 02/04/1998
• Issued: 07/13/1999
• Est. Priority Date: 02/07/1997
• Status: Expired due to Term

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Naya, Masayuki
• Primary Examiner(s): LE, QUE TAN
• Application Number:
  US09/018,332
• Time in Patent Office: 524 Days
• Field of Search: 250/227.25, 250/227.24, 250/227.14, 250/216, 356/244, 356/440, 356/445
• US Class Current: 250/227.25
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658

Abstract

A surface plasmon sensor comprises a sensor unit, which is provided with a transparent substrate and a metal film, and a coupler, which carries out light beam coupling and is separate from the sensor unit. A light beam is entered from a light beam entry portion of the coupler, and an attenuated total reflection angle θ_sp is detected from the light beam, which has been totally reflected from an interface between the transparent substrate and the metal film and has then been radiated out of a light beam radiating portion of the coupler. The sensor unit is supported by a sensor unit attachment such that a distance between the transparent substrate and the coupler may be kept to be equal to a predetermined value. The space between the transparent substrate and the coupler is filled with a refractive index matching liquid. Fitting of the sensor unit in the surface plasmon sensor is easy to carry out, and the relationship between positions of the sensor unit and the coupler can be kept constant.

Claims

1. A surface plasmon sensor, comprising:

i) a sensor unit provided with a transparent substrate, which has a predetermined refractive index, and a metal film, which is located on one surface side of said transparent substrate, and

ii) a coupler means located on the other surface side of said transparent substrate, which surface side is opposite to said one surface, with a refractive index matching liquid, which has a refractive index approximately equal to said predetermined refractive index, intervening between said transparent substrate and said coupler means,

said coupler means having a light beam entry portion, which is formed at a portion of said coupler means, and a light beam radiating portion, which is formed at a different portion of said coupler means, such that said coupler means transmit a light beam having been entered from said light beam entry portion, cause the transmitted light beam to impinge upon an interface between said transparent substrate and said metal film, transmit the light beam having been totally reflected from said interface, and then radiate the totally reflected light beam out of said light beam radiating portion, portions of said coupler means, which transmit the light beam, having a refractive index approximately equal to said predetermined refractive index,

the surface plasmon sensor causing the light beam to enter from said light beam entry portion and detecting an attenuated total reflection angle θ

_sp from the light beam, which has been totally reflected from said interface and has then been radiated out of said light beam radiating portion,

wherein a sensor unit support means is provided, said sensor unit support means supporting said sensor unit such that a distance between said transparent substrate and said coupler means be kept to be equal to a predetermined value, and

wherein the space between said transparent substrate and said coupler means is filled with said refractive index matching liquid.

2. A surface plasmon sensor as defined in claim 1 wherein said sensor unit support means is secured to a portion of said coupler means.

3. A surface plasmon sensor as defined in claim 1 wherein the surface plasmon sensor further comprises a matching liquid supply means, which supplies said refractive index matching liquid into said space, and

a vacant member, which communicates with said space and allows said refractive index matching liquid to be introduced up to a position higher than said other surface of said transparent substrate,

said refractive index matching liquid being filled in said space by said matching liquid supply means.

4. A surface plasmon sensor as defined in claim 1 wherein a liquid reservoir for storing said refractive index matching liquid therein is formed on the side of said coupler means, which side stands facing said transparent substrate,

said transparent substrate is provided with a waterproof wall, which surrounds said metal film, and

said sensor unit is supported in said liquid reservoir such that said other surface of said transparent substrate may be immersed in said refractive index matching liquid.

5. A surface plasmon sensor as defined in claim 1 wherein said coupler means is provided with a prism, and

said light beam entry portion and said light beam radiating portion are formed in said prism.

6. A surface plasmon sensor as defined in claim 1 wherein each of said light beam entry portion and said light beam radiating portion of said coupler means is constituted of a diffraction grating.

7. A surface plasmon sensor as defined in claim 1 wherein said coupler means is provided with a convex portion on the side of said coupler means, which side is opposite to the side facing said transparent substrate,

each of one lateral face of said convex portion and the other lateral face thereof, which is opposite to said one lateral face, is constituted of a transparent plate, the region inside of said convex portion being filled with said refractive index matching liquid, and

said one lateral face and said other lateral face of said convex portion respectively serve as said light beam entry portion and said light beam radiating portion.

8. A surface plasmon sensor as defined in claim 1 wherein said transparent substrate of said sensor unit is constituted of a main transparent substrate and a supporting transparent substrate, which have said predetermined refractive index and are in close contact with each other,

said metal film is located on said main transparent substrate, and

said supporting transparent substrate is located such that it stand facing said coupler means with said refractive index matching liquid intervening therebetween.

9. A surface plasmon sensor as defined in claim 8 wherein said main transparent substrate and said supporting transparent substrate are in close contact with each other via a refractive index matching liquid, which has a refractive index approximately equal to said predetermined refractive index.

10. A surface plasmon sensor as defined in claim 1 wherein a bonding reaction film is located on said metal film, and the surface plasmon sensor detects a specific substance, which is capable of undergoing a bonding reaction with said bonding reaction film.

11. A surface plasmon sensor, comprising:

i) a sensor unit provided with a transparent substrate, which has a predetermined refractive index, and a metal film, which is located on one surface side of said transparent substrate, and

ii) a coupler means located on the other surface side of said transparent substrate, which surface side is opposite to said one surface, with a refractive index matching liquid, which has a refractive index approximately equal to said predetermined refractive index, intervening between said transparent substrate and said coupler means,

said coupler means having a light beam entry portion, which is formed at a portion of said coupler means, and a light beam radiating portion, which is formed at a different portion of said coupler means, such that said coupler means transmit a light beam having been entered from said light beam entry portion, cause the transmitted light beam to impinge upon an interface between said transparent substrate and said metal film, transmit the light beam having been totally reflected from said interface, and then radiate the totally reflected light beam out of said light beam radiating portion, portions of said coupler means, which transmit the light beam, having a refractive index approximately equal to said predetermined refractive index,

the surface plasmon sensor causing the light beam to enter from said light beam entry portion and detecting an attenuated total reflection angle θ

_sp from the light beam, which has been totally reflected from said interface and has then been radiated out of said light beam radiating portion,

wherein a sensor unit support means is provided, said sensor unit support means supporting said sensor unit such that a distance between said transparent substrate and said coupler means be kept to be equal to a predetermined value,

wherein the space between said transparent substrate and said coupler means is filled with said refractive index matching liquid, and

wherein the surface plasmon sensor further comprises either one or both of an incidence position shifting means and a sensor unit relative movement means,

said incidence position shifting means shifting the incidence position of the light beam at said light beam entry portion such that the light beam successively impinge upon different portions of said interface, which are taken along a predetermined direction, and under the same incidence conditions,

said sensor unit relative movement means moving said sensor unit with respect to said coupler means and along a predetermined direction such that said distance between said transparent substrate and said coupler means be kept to be equal to the predetermined value.

12. A surface plasmon sensor as defined in claim 11 wherein the surface plasmon sensor comprises both of said incidence position shifting means and said sensor unit relative movement means, and

said predetermined direction, along which said different portions of said interface are taken in the shifting operation carried out by said incidence position shifting means, and said predetermined direction, along which said sensor unit is moved with respect to said coupler means by said sensor unit relative movement means, intersect with each other.

13. A surface plasmon sensor as defined in claim 11 wherein said incidence position shifting means is a telecentric scanning optical system.

14. A surface plasmon sensor as defined in claim 11 wherein said sensor unit support means is secured to a portion of said coupler means.

15. A surface plasmon sensor as defined in claim 11 wherein a light-source optical means for producing the light beam and causing the light beam to enter into said light beam entry portion, a detection means for detecting an attenuated total reflection angle θ

_sp from the light beam having been radiated out of said light beam radiating portion, and said coupler means are located on a base,

said sensor unit support means is located on said base and secured thereto,

said sensor unit is supported by said sensor unit support means such that said sensor unit can be moved with respect to said coupler means, and

said sensor unit relative movement means moves said sensor unit.

16. A surface plasmon sensor as defined in claim 11 wherein said sensor unit support means is located on a base and secured thereto,

said sensor unit is fixedly supported by said sensor unit support means,

an optical system unit is located on said base such that said optical system unit can be moved with respect to said sensor unit, said optical system unit comprising a light-source optical means for producing the light beam and causing the light beam to enter into said light beam entry portion, a detection means for detecting an attenuated total reflection angle θ

_sp from the light beam having been radiated out of said light beam radiating portion, and said coupler means, and

said sensor unit relative movement means moves said optical system unit.

17. A surface plasmon sensor as defined in claim 11 wherein said sensor unit support means is located on a base and secured thereto,

said sensor unit is supported by said sensor unit support means such that said sensor unit can be moved with respect to said coupler means,

said sensor unit relative movement means moves said sensor unit,

an optical system unit is located on said base, said optical system unit comprising an optical means for a light source, which optical means causes the light beam to enter into said light beam entry portion, an optical means for a detection means, which optical means guides the light beam to the detection means for detecting an attenuated total reflection angle θ

_sp from the light beam having been radiated out of said light beam radiating portion, and said coupler means, and

the light source for producing the light beam and said detection means are releasably located on said base and at positions independent of said optical system unit.

18. A surface plasmon sensor as defined in claim 11 wherein said sensor unit support means is located on a base and secured thereto,

said sensor unit is fixedly supported by said sensor unit support means,

an optical system unit is located on said base such that said optical system unit can be moved with respect to said sensor unit, said optical system unit comprising an optical means for a light source, which optical means causes the light beam to enter into said light beam entry portion, an optical means for a detection means, which optical means guides the light beam to the detection means for detecting an attenuated total reflection angle θ

_sp from the light beam having been radiated out of said light beam radiating portion, and said coupler means,

said sensor unit relative movement means moves said optical system unit, and

the light source for producing the light beam and said detection means are releasably located on said base and at positions independent of said optical system unit.

19. A surface plasmon sensor as defined in claim 11 wherein said sensor unit support means is located on a base and secured thereto,

said sensor unit is supported by said sensor unit support means such that said sensor unit can be moved with respect to said coupler means,

said sensor unit relative movement means moves said sensor unit,

an optical system unit is located on said base, said optical system unit comprising an optical means for a light source, which optical means causes the light beam to enter into said light beam entry portion, a detection means for detecting an attenuated total reflection angle θ

_sp from the light beam having been radiated out of said light beam radiating portion, and said coupler means, and

the light source for producing the light beam is independently located at the exterior of said base.

20. A surface plasmon sensor as defined in claim 11 wherein said sensor unit support means is located on a base and secured thereto,

said sensor unit is fixedly supported by said sensor unit support means,

an optical system unit is located on said base such that said optical system unit can be moved with respect to said sensor unit, said optical system unit comprising an optical means for a light source, which optical means causes the light beam to enter into said light beam entry portion, a detection means for detecting an attenuated total reflection angle θ

_sp from the light beam having been radiated out of said light beam radiating portion, and said coupler means,

said sensor unit relative movement means moves said optical system unit, and

the light source for producing the light beam is independently located at the exterior of said base.

21. A surface plasmon sensor as defined in claim 19 wherein the surface plasmon sensor is provided with an optical fiber for guiding the light beam, which has been produced by said light source, to said optical means for the light source.

22. A surface plasmon sensor as defined in claim 20 wherein the surface plasmon sensor is provided with an optical fiber for guiding the light beam, which has been produced by said light source, to said optical means for the light source.

23. A surface plasmon sensor as defined in claim 21 wherein said optical fiber is a polarization plane keeping type of optical fiber.

24. A surface plasmon sensor as defined in claim 22 wherein said optical fiber is a polarization plane keeping type of optical fiber.

25. A surface plasmon sensor as defined in claim 11 wherein the surface plasmon sensor further comprises a matching liquid supply means, which supplies said refractive index matching liquid into said space, and

a vacant member, which communicates with said space and allows said refractive index matching liquid to be introduced up to a position higher than said other surface of said transparent substrate,

said refractive index matching liquid being filled in said space by said matching liquid supply means.

26. A surface plasmon sensor as defined in claim 11 wherein a liquid reservoir for storing said refractive index matching liquid therein is formed on the side of said coupler means, which side stands facing said transparent substrate,

said transparent substrate is provided with a waterproof wall, which surrounds said metal film, and

said sensor unit is supported in said liquid reservoir such that said other surface of said transparent substrate be immersed in said refractive index matching liquid.

27. A surface plasmon sensor as defined in claim 11 wherein said coupler means is provided with a prism, and

said light beam entry portion and said light beam radiating portion are formed in said prism.

28. A surface plasmon sensor as defined in claim 11 wherein each of said light beam entry portion and said light beam radiating portion of said coupler means is constituted of a diffraction grating.

29. A surface plasmon sensor as defined in claim 11 wherein said coupler means is provided with a convex portion on the side of said coupler means, which side is opposite to the side facing said transparent substrate,

each of one lateral face of said convex portion and the other lateral face thereof, which is opposite to said one lateral face, is constituted of a transparent plate, the region inside of said convex portion being filled with said refractive index matching liquid, and

said one lateral face and said other lateral face of said convex portion respectively serve as said light beam entry portion and said light beam radiating portion.

30. A surface plasmon sensor as defined in claim 11 wherein said transparent substrate of said sensor unit is constituted of a supporting transparent substrate and a main transparent substrate, which is located on said supporting transparent substrate,

said metal film is located on said main transparent substrate, and

said supporting transparent substrate is located such that it stand facing said coupler means with said refractive index matching liquid intervening therebetween.

31. A surface plasmon sensor as defined in claim 11 wherein a bonding reaction film is located on said metal film, and the surface plasmon sensor detects a specific substance, which is capable of undergoing a bonding reaction with said bonding reaction film.

32. A surface plasmon sensor as defined in claim 11 wherein different kinds of bonding reaction films are located at different positions on said metal film, and the surface plasmon sensor detects a specific substance, which is capable of undergoing a bonding reaction with each of said bonding reaction films.

33. A surface plasmon sensor as defined in claim 11 wherein said transparent substrate of said sensor unit is constituted of a supporting transparent substrate and a plurality of main transparent substrates, which are located at different positions on said supporting transparent substrate,

metal films are located respectively on the plurality of said main transparent substrates, and

said supporting transparent substrate is located such that it stand facing said coupler means with said refractive index matching liquid intervening therebetween.

34. A surface plasmon sensor as defined in claim 33 wherein the plurality of said main transparent substrates have different substrate sizes.

35. A surface plasmon sensor as defined in claim 33 wherein bonding reaction films are located respectively on said metal films, each of which is located on one of said main transparent substrates, and the surface plasmon sensor detects a specific substance, which is capable of undergoing a bonding reaction with each of said bonding reaction films.

36. A surface plasmon sensor as defined in claim 35 wherein said bonding reaction films are different kinds of reaction films.

37. A surface plasmon sensor as defined in claim 30 wherein said supporting transparent substrate and said main transparent substrate are in close contact with each other via a refractive index matching liquid, which has a refractive index approximately equal to said predetermined refractive index.

38. A surface plasmon sensor as defined in claim 33 wherein said supporting transparent substrate and each of said main transparent substrates are in close contact with each other via a refractive index matching liquid, which has a refractive index approximately equal to said predetermined refractive index.

39. A surface plasmon sensor as defined in claim 30 wherein said main transparent substrate is releasably located on said supporting transparent substrate.

40. A surface plasmon sensor as defined in claim 33 wherein said main transparent substrates are releasably located on said supporting transparent substrate.

41. A surface plasmon sensor, comprising:

i) a sensor unit provided with a transparent substrate, which has a predetermined refractive index, and a metal film, which is located on one surface side of said transparent substrate and brought into contact with a sample,

ii) a coupler means located on the other surface side of said transparent substrate, which surface side is opposite to said one surface, with a refractive index matching liquid, which has a refractive index approximately equal to said predetermined refractive index, intervening between said transparent substrate and said coupler means,

said coupler means having a light beam entry portion, which is formed at a portion of said coupler means, and a light beam radiating portion, which is formed at a different portion of said coupler means, such that said coupler means transmit a light beam having been entered from said light beam entry portion, cause the transmitted light beam to impinge upon an interface between said transparent substrate and said metal film, transmit the light beam having been totally reflected from said interface, and then radiate the totally reflected light beam out of said light beam radiating portion, portions of said coupler means, which transmit the light beam, having a refractive index approximately equal to said predetermined refractive index, and

iii) a light source for producing the light beam,

the surface plasmon sensor collimating the light beam, which has been produced by said light source, causing the collimated light beam to enter from said light beam entry portion, and detecting an attenuated total reflection angle θ

_sp from the light beam, which has been totally reflected from said interface and has then been radiated out of said light beam radiating portion,

wherein each of said light beam entry portion and said light beam radiating portion is constituted of a hologram optical element.

42. A surface plasmon sensor as defined in claim 41 wherein said hologram optical element is designed to converge the incident collimated light beam to a predetermined position,

a light source moving means is provided, said light source moving means moving said light source in a plane, which is parallel to said interface, and in a direction heading towards said predetermined position or in a direction heading away from said predetermined position, and

said light source is moved, whereby the light beam is caused to enter from different positions on said light beam entry portion and caused to impinge upon said interface at various different angles of incidence and at said predetermined position.

43. A surface plasmon sensor as defined in claim 42 wherein said hologram optical element has lens functions and aberration compensating functions, and the light beam is converged to said predetermined position by said lens functions and said aberration compensating functions.

44. A surface plasmon sensor as defined in claim 42 wherein said hologram optical element has cylindrical lens functions and aberration compensating functions, and the light beam is converged to said predetermined position by said cylindrical lens functions and said aberration compensating functions.

45. A surface plasmon sensor as defined in claim 44 wherein the surface plasmon sensor further comprises a position regulating means for regulating the position of said sensor unit such that a distance between said transparent substrate and said coupler means be kept to be equal to a predetermined value, and

a sensor unit moving means for moving said sensor unit in a predetermined direction,

the space between said transparent substrate and said coupler means being filled with said refractive index matching liquid.

46. A surface plasmon sensor as defined in claim 44 wherein the surface plasmon sensor further comprises an incidence position shifting means for shifting the incidence position of the light beam at said light beam entry portion such that the light beam may successively impinge upon different portions of said interface, which are taken along the same direction as an axial direction of said cylindrical lens functions.