Method of identifying nucleotide differences
First Claim
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1. A method of identifying nucleotide differences between the sequence of a target nucleic acid and the sequence of a reference nucleic acid comprising:
- a) providing a substrate having at least 1000 different polynucleotide probes of known sequence at known locations, attached at a density of at least 10,000 probes per square cm;
b) contacting the target nucleic acid with the polynucleotide probes attached to the substrate under conditions for high specificity complementary hybridization;
c) determining which polynucleotide probes have hybridized with the target nucleic acid; and
d) using a computer to (i) compare the sequence of the reference nucleic acid with the sequences of the polynucleotide probes that have hybridized with the target nucleic acid and (ii) identify the nucleotide differences between the sequence of the target nucleic acid and the sequence of the reference nucleic acid.
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Abstract
The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and to characterize their sources.
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Citations
6 Claims
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1. A method of identifying nucleotide differences between the sequence of a target nucleic acid and the sequence of a reference nucleic acid comprising:
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a) providing a substrate having at least 1000 different polynucleotide probes of known sequence at known locations, attached at a density of at least 10,000 probes per square cm; b) contacting the target nucleic acid with the polynucleotide probes attached to the substrate under conditions for high specificity complementary hybridization; c) determining which polynucleotide probes have hybridized with the target nucleic acid; and d) using a computer to (i) compare the sequence of the reference nucleic acid with the sequences of the polynucleotide probes that have hybridized with the target nucleic acid and (ii) identify the nucleotide differences between the sequence of the target nucleic acid and the sequence of the reference nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification