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Bacterial strains which overproduce riboflavin

  • US 5,925,538 A
  • Filed: 08/24/1998
  • Issued: 07/20/1999
  • Est. Priority Date: 06/22/1989
  • Status: Expired due to Fees
First Claim
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1. A method for production of riboflavin comprising, providing a recombinant bacterium comprising an exogenously introduced nucleic acid sequence, which sequence comprises a bacterial ORF3 structural gene and an exogenous promoter, said promoter being positioned to transcribe said ORF3 structural gene, said ORF3 structural gene encoding an amino acid sequence that has ORF3 activity, hybridizing to B. subtilis rib ORF3;

  • culturing said recombinant bacterium under suitable culture conditions such that said ORF3 structural gene is expressed and the recombinant bacterium produces a riboflavin titre of at least 10 g/l when fermented using standard commercial batch and feed conditions; and

    recovering riboflavin produced by said bacterium.

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