Blood test for assessing hepatic function

• US 5,928,167 A
• Filed: 10/20/1997
• Issued: 07/27/1999
• Est. Priority Date: 10/20/1997
• Status: Expired due to Fees

• Current Assignee: Metabolic Solutions Incorporated
• Original Assignee: Metabolic Solutions Incorporated
• Inventors: Wagner, David A., Woolf, Graham M.
• Primary Examiner(s): Hindenburg, Max
• Assistant Examiner(s): MARMOR II, CHARLES ALAN
• Application Number:
  US08/953,893
• Time in Patent Office: 645 Days
• Field of Search: 600/573, 600/584, 128/898
• US Class Current: 600/584
• CPC Class Codes: G01N 2800/08 Hepato-biliairy disorders other than hepatitis
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere

Abstract

Provided herein is a novel blood test for assessing hepatic function. The test involves administration of a labeled methionine or methionine metabolite to a subject and measurement of the expired label in blood.

Claims

1. A method of assessing hepatic mitochondrial function in a subject comprising the steps of:

a) administering to said subject an effective amount of carbon-labeled methionine or methionine metabolite to said subject;

b) collecting a blood specimen from said subject; and

c) measuring the amount of labeled carbon in said blood specimen to assess hepatic mitochondrial function in said subject.

2. The method according to claim 1 wherein said carbon-labeled methionine or methionine metabolite is selected from the group consisting of ¹⁴ C methionine or methionine metabolite and ¹³ C methionine or methionine metabolite, or mixtures thereof.

3. The method according to claim 1 wherein said carbon-labeled methionine is ¹³ C methionine.

4. The method according to claim 1 wherein said carbon-labeled methionine or methionine metabolite is labeled at the 1-position of methionine or the 1-position of the methionine metabolite.

5. The method of claim 1 wherein said carbon-labeled methionine or methionine metabolite comprises a plurality of labeled carbons.

6. The method according to claim 1 wherein said labeled carbon in said blood is labeled carbon dioxide.

7. The method according to claim 6 wherein said labeled carbon dioxide is ¹³ C carbon dioxide.

8. The method according to claim 1 wherein said administering step comprises administering carbon-labeled methionine or methionine metabolite in a pharmaceutically acceptable carrier.

9. The method according to claim 1 further comprising comparing said amount of expired labeled carbon with a standard, whereby said comparing yields a measure of hepatic mitochondrial function.

10. The method according to claim 9 wherein said standard comprises the mean value of expired label in a control population without hepatic disease or hepatic dysfunction.

11. The method according to claim 1 or claim 9 wherein said assessing mitochondrial function is used to diagnose hepatic disease or hepatic dysfunction.

12. The method according to claim 11 wherein said hepatic disease or dysfunction is selected from the group consisting of chronic liver disease, fulminant hepatic failure, viral-induced liver disease, metabolic liver disease, and hepatic dysfunction associated with sepsis or liver trauma.

13. The method according to claim 1 wherein said measuring comprises isotopic measurement of labeled carbon.

14. The method according to claim 13 wherein said measurement is selected from the group consisting of mass spectrometric measurement, laser measurement, infrared detection, nuclear magnetic resonance and liquid scintillation counting of radioactive carbon.

15. The method according to claim 1 wherein said blood specimen is selected from the group consisting of venous, capillary and arterial blood.

16. The method according to claim 15 wherein said blood specimen is treated with an acid prior to analysis of CO₂.

17. The method according to claim 16 wherein said acid is citric acid.

18. The method of claim 1 wherein said carbon-labeled methionine metabolite is selected from the group consisting of carbon-labeled S-adenosylmethionine, S-adenosylhomocysteine, homocysteine, cystathionine, homoserine and α

-ketobutyrate.

19. A kit for assessing hepatic mitochondrial function comprising carbon-labeled methionine or methionine metabolite in a pharmaceutically acceptable carrier.

20. The kit of claim 19 further comprising a means for collecting blood.

21. The kit of claim 20 wherein said means for collecting blood is a Vacutainer tube and an acid.

22. The kit of claim 21 wherein said acid is citric acid.