Compositions for neutralization of lipopolysaccharides
First Claim
1. A method for monitoring or prognoisis of a subject suffering from gram-negative or endotoxin-mediated sepsis comprising:
- a) measuring a first level of lipoprotein particles that contain a lipid exchange protein that is characterized by being capable of facilitating an exchange of lipopolysaccharide into the high density lipoprotein particles in a biological fluid of the subject, wherein the high density lipoprotein particles comprise lipoprotein A-I, and the lipid exchange protein is lipopolysaccharide binding protein;
b) measuring a second level of lipoprotein particles that contain a lipid exchange protein that is characterized by being capable of faciliating an exchange of lipopolysaccharide into the high density lipoprotein particles in the biological fluid of the subject, wherein the high density lipoprotein particles comprise lipoprotein A-I, and the lipid exchange protein is lipopolysaccharide binding protein; and
c) comparing the second level measured in step (h) with the first level measured in step (a), wherein an increase in the level indicates greater probability of a favorable outcome of the sepsis.
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Abstract
The present invention relates to compositions and methods for neutralizing lipopolysaccharide, and treatment of gram-negative sepsis based thereon. Accordingly, the invention is directed to a composition of homogeneous particles comprising phospholipids and a lipid exchange protein, such as phospholipid transfer protein or LPS binding protein. The lipid exchange protein is characterized by being capable of faciliting an exchange of lipopolysaccharide into the particles. In a specific embodiment, exemplified herein, the lipid particles are high density lipoprotein particles comprising apolipoprotein A-I (apo A-I), a phospholipid, and cholesterol or a lipid bilayer binding derivative thereof. In a specific example, the phospholipid is phosphatidylcholine (PC). In a specific example, the ratio of phosphatidylcholine: cholesterol: apolipoprotein A-I is approximately 80:4:1. The level of LPS exchange protein activity in a sample from a patient provides a diagnostic, monitoring, or prognostic indicator for a subject with endotoxemia, gram-negative sepsis, or septic shock.
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Citations
12 Claims
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1. A method for monitoring or prognoisis of a subject suffering from gram-negative or endotoxin-mediated sepsis comprising:
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a) measuring a first level of lipoprotein particles that contain a lipid exchange protein that is characterized by being capable of facilitating an exchange of lipopolysaccharide into the high density lipoprotein particles in a biological fluid of the subject, wherein the high density lipoprotein particles comprise lipoprotein A-I, and the lipid exchange protein is lipopolysaccharide binding protein; b) measuring a second level of lipoprotein particles that contain a lipid exchange protein that is characterized by being capable of faciliating an exchange of lipopolysaccharide into the high density lipoprotein particles in the biological fluid of the subject, wherein the high density lipoprotein particles comprise lipoprotein A-I, and the lipid exchange protein is lipopolysaccharide binding protein; and c) comparing the second level measured in step (h) with the first level measured in step (a), wherein an increase in the level indicates greater probability of a favorable outcome of the sepsis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification