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Mesoscale sample preparation device and systems for determination and processing of analytes

  • US 5,928,880 A
  • Filed: 06/11/1997
  • Issued: 07/27/1999
  • Est. Priority Date: 05/01/1992
  • Status: Expired due to Term
First Claim
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1. A method of separating a target subpopulation of cells in a cell-containing sample fluid comprising the steps of:

  • a) providing a solid substrate microfabricated to define a mesoscale sample fluid flow passage in fluid communication with a mesoscale chamber, said chamber and said flow passage being dissimilar in their cross-sectional dimensions, said chamber having a wall portion defining an enclosure in which is disposed an immobilized binding protein specific for a cell membrane-bound protein characteristic of said target population;

    b) passing a cell-containing sample fluid through said enclosure under conditions to permit capture of members of the cell target subpopulation by reversible cell surface protein-immobilized protein binding, while permitting other cells to pass therethrough; and

    c) controlling the environment in said enclosure to effect release of said target subpopulation of cells.

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