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Methods and compositions for transforming cells

  • US 5,928,914 A
  • Filed: 11/05/1996
  • Issued: 07/27/1999
  • Est. Priority Date: 06/14/1996
  • Status: Expired due to Term
First Claim
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1. A method of causing site-specific recombination in a mammalian cell comprising contacting a recombinase with (a) an acceptor vector which is integrated into the genome of said mammalian cell, said acceptor vector comprising two lox sequences, L1 and L2, which can not recombine with one another, and (b) a donor vector which is not integrated into the genome of said mammalian cell, said donor vector being present in an amount in excess of said integrated acceptor vector, wherein said donor vector comprises a selected DNA flanked by the same L1 and L2 sequences contained in the acceptor vector, or sequences which can recombine with these L1 and L2 sequences, thereby causing transfer of the selected DNA from the donor vector into the acceptor vector by recombination at the L1 and L2 sequences.

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