Quality control for biological decontamination and sterilization

• US 5,932,173 A
• Filed: 10/10/1997
• Issued: 08/03/1999
• Est. Priority Date: 10/03/1996
• Status: Expired due to Fees

• Current Assignee: Microbiology Specialists Incorporated
• Original Assignee: Microbiology Specialists Incorporated
• Inventors: Weissfeld, Alice, Vance, Paula
• Primary Examiner(s): Phasge, Arun S.
• Application Number:
  US08/949,094
• Time in Patent Office: 662 Days
• Field of Search: 435/31, 435/4, 435/34, 435/810, 422/61
• US Class Current: 422/401
• CPC Class Codes: C12Q 1/22 Testing for sterility conditions
• CPC Class Codes: C12Q 1/24 Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

The present invention relates to new methods and new destruction-resistant materials for simple and routine quality control measurement of the effectiveness of biological decontamination and sterilization processes that utilize at least one step involving physical disruption of treated wastes, where such physical disruption would destroy conventional, non-destruction-resistant quality control means. Quantitative and qualitative analysis is provided.

Claims

1. An article for quality control in infectious waste treatment systems comprising:

a destruction resistant medium, said medium containing indicator microorganisms at a given number per unit, said microorganisms being homogeneously seeded throughout the entirety of said medium, such that the number of microorganisms per given unit of medium is substantially constant throughout the entirety of the medium;

said medium being non-brittle and resilient;

said medium being severable, such that said medium is severable into at least two separate pieces upon treatment of said medium in a waste treatment process having a physical disruption step; and

said medium containing a known kind of microorganisms.

2. The article of claim 1 wherein said medium is resistant to physical manipulation and resisting breakage into multiple pieces upon physical manipulation.

3. The article of claim 1 wherein said medium is distinctly marked to visually stand out from treated infectious waste.

4. The article of claim 3 wherein said marked medium is colored.

5. The article of claim 3 wherein said medium is distinctly marked medium being of a pattern sufficient to distinctly stand out from treated infectious waste.

6. The article of claim 1 wherein said medium is resistant to heat disinfection.

7. The article of claim 1 wherein said medium is resistant to chemical disinfection.

8. The article of claim 1, where the destruction resistant medium is a sponge.

9. The article of claim 8, where the sponge is a cellulose and nylon sponge.

10. The article of claim 1, wherein the indicator microorganisms are selected from the group consisting of B. subtilis, B. stearothermophilus and M. bovis.