Microfluidic structure assembly with mating microfeatures

• US 5,932,315 A
• Filed: 04/30/1997
• Issued: 08/03/1999
• Est. Priority Date: 04/30/1997
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Hewlett-Packard Company (HP Inc.)
• Inventors: Greenstein, Michael, Lum, Paul
• Primary Examiner(s): Loney, Donald
• Application Number:
  US08/846,570
• Time in Patent Office: 825 Days
• Field of Search: 428/156, 428/167, 428/172, 428/192, 428/212, 428/178, 428/188, 428/120, 264/138, 264/241, 264/166, 264/167, 264/297.1, 264/220, 264/250, 264/299, 261/108, 261/110, 210/151, 210/294, 210/322, 156/290, 156/292, 156/308.4, 156/244.19, 156/250, 156/297
• US Class Current: 428/172
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B29C 2793/009 after shaping
• CPC Class Codes: B29C 65/02 by heating, with or without pressure
• CPC Class Codes: B29C 65/56 using mechanical means or mechanical connections, e.g. form-fits
• CPC Class Codes: B29C 65/7814 in the form of inter-cooperating positioning features holding or clamping means cooperating with specially formed features of at least one of the parts to be joined B29C65/7844, e.g. tenons and mortises tenon and mortise joints B29C66/126; tongue and groove joints B29C66/124
• CPC Class Codes: B29C 66/1122 Single lap to lap joints, i.e. overlap joints B29C66/45, B29C66/472, B29C66/52272 take precedence
• CPC Class Codes: B29C 66/54 Joining several hollow-preforms, e.g. half-shells, to form hollow articles, e.g. for making balls, containers; Joining several hollow-preforms, e.g. half-cylinders, to form tubular articles
• CPC Class Codes: B29C 66/71 characterised by the composition of the plastics material of the parts to be joined welding bar compositions B29C65/125
• CPC Class Codes: B29C 66/73921 characterised by the materials of both parts being thermoplastics
• CPC Class Codes: B29C 66/73941 characterised by the materials of both parts being thermosets
• CPC Class Codes: B29C 66/8322 Joining or pressing tools reciprocating along one axis
• CPC Class Codes: B29K 2079/08 PI, i.e. polyimides or derivatives thereof
• CPC Class Codes: B29L 2031/756 Microarticles, nanoarticles
• CPC Class Codes: B32B 3/30 characterised by a layer formed with recesses or projections, e.g. hollows, grooves, protuberances, ribs apertured layer B32B3/266; layer with cavities or internal voids B32B3/26
• CPC Class Codes: B81B 2201/058 Microfluidics not provided for in B81B2201/051 - B81B2201/054
• CPC Class Codes: B81C 2203/054 using structural alignment aids, e.g. spacers, interposers, male/female parts, rods or balls
• CPC Class Codes: B81C 3/005 : Passive alignment, i.e. without a detection of the position of the elements or using only structural arrangements or thermodynamic forces
• CPC Class Codes: G01N 27/44791 : Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: G01N 30/6095 : Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: Y10T 156/1089 : of discrete laminae to single face of additional lamina
• CPC Class Codes: Y10T 428/24479 : including variation in thickness
• CPC Class Codes: Y10T 428/2457 : Parallel ribs and/or grooves
• CPC Class Codes: Y10T 428/24612 : Composite web or sheet
• CPC Class Codes: Y10T 428/24661 : Forming, or cooperating to form cells
• CPC Class Codes: Y10T 428/24744 : Longitudinal or transverse tubular cavity or cell

Abstract

A microfluidic structure assembly having a microchannel formed by bonding two plates together is disclosed. The microfluidic structure assembly includes a first plate and a second plate. At least one of these plates has one or more microgrooves. Microdepressions and microprojections are also present in the plates and they connect such that the microprojections of one plate fit into the microdepressions of the other plate. As a result, the two plates are proximate to each other to form an assembly in which the microgrooves to form microchannels. These microdepressions and microprojections securely lock the microchannels into desired positions. Preferably the plates are made by molding to form the microdepressions, microgrooves, and microchannels.

Claims

1. A microfluidic structure assembly, comprising:

(a) a first plate including a plurality of microdepressions on a mating side of the first plate; and

(b) a second plate including a plurality of microprojections on a mating side of the second plate, coupling to said plurality of microdepressions of the first plate such that at least some of the microprojections of one plate fit into at least some of the microdepressions of the other plate sufficiently far to lock the plates from sliding to form an assembly;

wherein in the assembly at least one of the two plates comprising on its mating side microgrooves neighboring to microprojections and microdepressions, the microgrooves not being mated with any microprojections, such that the microgrooves of one plate are covered by the other plate to form microchannels, such that the assembly can be divided into a plurality of identical smaller assemblies each having one or more identical microchannels.

2. A microfluidic structure assembly, comprising:

(a) a first plate having a mating side and having on the mating side a plurality of microdepressions; and

(b) a second plate having a mating side for coupling with the first plate'"'"'s mating side, the second plate'"'"'s mating side having a plurality of microprojections such that the microprojections of one plate fit into the microdepressions of the other plate sufficiently far to lock the two plates from sliding to form an assembly;

wherein in the assembly at least one of the two plates comprising on its mating side one or more microgrooves neighboring microdepressions and microprojections, the microgrooves not mating with any mircroprojections, such that the microgrooves of one plate are covered by the other plate to form microchannels;

where in the microdepressions, microgrooves, and microchannels and are made by molding.

3. The assembly according to claim 2 wherein the microdepressions are smaller and shallower than and at least ten times as numerous as the microgrooves, and at least two of the microdepressions are nearer to the mid point than to an edge on the plate on which the microdepressions are located.

4. The assembly according to claim 2 wherein each plate comprises one or more microgrooves matching the microgrooves of the other plate to form microchanels in the assembly such that a microchannel has a cross section that is the sum of the cross sections of the two matching microgrooves.

5. The assembly according to claim 2 wherein the two coupled plates form a plurality of identical microchannels in the assembly such that the assembly can be divided to result in a plurality of identical pieces each including a microchannel.

6. The assembly according to claim 2 wherein the first and the second plate each comprises a plurality of microgrooves matching a plurality of microgrooves of the other plate to form microchannels in the assembly such that each of the microchannels has a cross section that is the sum of the cross sections of the two matching microgrooves, such that the assembly can be cut to result in a plurality of identical pieces each including a microchannel.

7. The assembly according to claim 6 wherein at least one plate further includes one or more cavities connected to the microchannels enabling fluid communication therewith, said cavities having a volume substantially larger than that of the microchannel.

8. The assembly according to claim 7 wherein the one or more cavities contain one or more chemicals for reacting with an analyte in a fluid to be passed through a microchannel to the one or more cavities.

9. The assembly according to claim 2 wherein the first plate and the second plate each comprises a plurality of microgrooves matching the microgrooves of the other plate to form microchannels in the assembly such that each microchannel has a cross section that is the sum of the cross sections of the two matching microgrooves, the microdepressions are smaller and shallower than and are at least ten times as numerous as the microgrooves, at least four of the microdepressions are nearer to a mid point than to an edge on the plate on which the microdepressions are located, the first and second plates are made of a thermoset polymer and are bonded together, such that the assembly can be cut to result in a plurality of identical pieces each including a microchannel.

10. The assembly according to claim 2 wherein each plate comprises both microprojections and microdepressions that mate with the microprojections and microdepressions of the other plate to lock the plates from sliding.

11. A method of making a microfluidic structure assembly, comprising:

(a) making a mold by micromachining one side of a substrate to form on the substrate a plurality of identical microridges and a plurality of microfingers, the microfingers having a height smaller than and at least ten times as numerous as the microridges, and at least ten of the microfingers are nearer to a mid point than to an edge on the mold on which the microfingers are located, at least some of the microfingers being positioned along the microridges proximate thereto;

(b) depositing on the mold a layer of liquid for forming a polymeric material to cover the microridges and cover said plurality of microfingers and allowing the liquid for forming a polymeric material to solidify to form a molded plate;

(c) releasing the molded plate from the mold such that the solidified plate has one or more microgrooves corresponding to the microridges on the mold, and a plurality of microdepressions corresponding to the microfingers, the molded plate being a first plate of an assembly;

(d) making a second molded plate to form a second plate of the assembly, the second plate including microgrooves mirroring the microgrooves of the first molded plate and microprojections positioned mirroring the positions of the microdepressions of the first plate, such that microdepressions of one molded plate correspond to microprojections of the other plate;

(e) bonding the two molded plates to form the assembly, said plurality of microdepressions and plurality of microprojections of the two plates coupling such that the microprojections of one plate fit sufficiently far into the microdepressions of the other plate to lock the plates from sliding such that the plates are proximate to each other for the microgrooves of the two plates to match to form microchannels for fluid flow; and

(a) dividing the assembly with the first and second plates to result in a plurality of identical smaller assemblies each including a microchannel.

12. A method of making a mnicrofluidic structure assembly, comprising:

(a) making a first mold by micromachining one side of a first substrate to form on the first substrate a plurality of microholes;

(b) making a second mold by micromaching one side of the second substrate to form on the second substrate a plurality of microfingers with positions mirroring to the microholes of the first substrate;

(c) micromachining one or more microridges on at least one of the first substrate and the second substrate, the microridges not having positions mirroring to any microhole of another plate;

(d) depositing on the machined side of each mold a layer of liquid for forming a polymeric material to cover any machined microridges, microfingers and microholes, and allowing the liquid to solidify on each substrate to form a molded plate such that any microridge on the mold will form a microgroove on the molded plate, any microfinger will form a microdepression on the molded plate and any microhole will form a microprojection on the molded plate;

(e) releasing the molded plates from the molds; and

(f) bonding a molded plate from the first substrate with a molded plate from the second substrate such that the two plates form an assembly, the microdepressions and the microprojections of the two plates are coupled by the microprojections of one plate fitting sufficiently far into the microdepressions of the other plate to lock from sliding, the plates are proximate to each other for the microgrooves to form microchannels for fluid flow.

13. The method according to claim 12 further comprising making the microdepressions to be smaller and shallower than and at least ten times as numerous as the microgrooves, and at least two of the microdepressions are nearer to a mid point than to an edge on the plate on which the microdepressions are located.

14. The method according to claim 12 further cormprising making one or more microgrooves on each plate to match one or more microgrooves on the other plate to form the microchannels such that a microchanmel has a cross section that is the sum of the cross sections of two matching microgrooves that face each other.

15. The method according to claim 12 further comprising making the first and second plates with a thermoset polymer and fastening them together by an adhesive.

16. The method according to claim 12 further comprising making the microdepressions of one plate adequately larger than the microprojections of the other plate such that pressing the two plates together will force excess adhesive into the microdepressions, and further comprising pressing the plates and curing the adhesive by heating.

17. The method according to claim 12 further comprising lapping a plate of the assembly on a side facing away from the microgrooves to obtain a flat surface on the plate.

18. The method according to claim 12 further comprising making the two plates of the assembly such that a plurality of identical microchannels are formed such that the assembly can be cut to result in a plurality of identical smaller assemblies each including a microchannel.

19. The method according to claim 18 further comprising dividing the assembly with the first and second plates to result in a plurality of identical smaller assemblies each including a microchannel.

20. The method according to claim 12 further comprising making microgrooves in each plate to match microgrooves of the other plate to form the microchannels such that a microchannel has a cross section that is the sum of the cross sections of the two matching microgrooves, making the microdepressions to be smaller and shallower than and at least ten times as numerous as the microgrooves, making the two plates of the assembly such that a plurality of identical microchannels are formed such that the assembly can be cut to result in a plurality of identical smaller assemblies, and dividing the assembly with the first and second plates to result in a plurality of identical smaller assemblies each including a microchannel.

21. The method according to claim 12 wherein both microdepressions and microprojections are made in each plate and microprojections on one plate are fitted into microdepressions of the other plate to align the two plates.

22. The method according to claim 12 further comprising depositing a release layer on the mold before depositing the liquid on the mold.