Method and apparatus for accurately measuring the transmittance of blood within a retinal vessel

• US 5,935,076 A
• Filed: 02/10/1997
• Issued: 08/10/1999
• Est. Priority Date: 02/10/1997
• Status: Expired due to Term

• Current Assignee: Vectranetics, Inc.
• Original Assignee: University of Alabama in Huntsville (University of Alabama System)
• Inventors: Chipman, Russell A., Minnich, Thomas E., Denninghoff, Kurt R., Hillman, Lloyd W., Smith, Matthew H.
• Primary Examiner(s): Lateef, Marvin M.
• Assistant Examiner(s): MANTIS MERCADER, ELENI M
• Application Number:
  US08/797,414
• Time in Patent Office: 911 Days
• Field of Search: 600/479, 600/504, 600/558, 600/318, 600/319, 600/320, 600/310, 356/41, 351/206, 351/221
• US Class Current: 600/479
• CPC Class Codes: A61B 3/1233 for measuring blood flow, e.g. at the retina

Abstract

The method and apparatus for measuring the transmittance of blood within a retinal vessel detects the intensity of light reflected from illuminated portions of an eye, including the retinal vessel and background fundus, and adjusts the corresponding intensity signals to compensate for reflections from the retinal vessel. In particular, the transmittance measuring method and apparatus constructs an intensity profile function based upon the intensity signals. As a result, the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position. During the construction of the intensity profile function, the transmittance measuring method and apparatus can compensate for at least some of the reflections of light from the retinal vessel which occurred prior to propagation of the light through the retinal vessel, thereby increasing the accuracy with which the transmittance of blood within a retinal vessel is measured. In addition, the transmittance measuring method and apparatus can compensate for the finite point spread function of the light with which the eye is illuminated in order to further increase the resulting measurement accuracy.

Claims

1. A method of measuring the transmittance of blood within a retinal vessel, the method comprising the steps of:

illuminating the retinal vessel and at least a portion of the background fundus within an eye;

detecting the intensity of light reflected from illuminated portions of the eye;

identifying a portion of the detected light intensities as corresponding to light that has been transmitted through a retinal vessel, wherein said identifying step comprises matching a portion of the detected light intensities with a predetermined pattern corresponding to the intensity of light that has propagated through a retinal vessel;

constructing an intensity profile function based upon the identified portion of the detected light intensities, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, and wherein said constructing step comprises compensating for at least some of the reflections of light from the retinal vessel which occurred prior to propagation of the light through the retinal vessel; and

determining the transmittance of blood within the retinal vessel based upon the intensity profile function.

2. A method according to claim 1 wherein said constructing step further comprises determining the intensity of light transmitted through lateral portions of the retinal vessel, wherein said compensating step comprises compensating for reflections of light from a central portion of the retinal vessel which occurred prior to propagation of the light through the retinal vessel, and wherein said compensating step further comprises creating the intensity profile function based upon the intensity of light transmitted through the lateral portions of the retinal vessel such that the intensity profile function approximates the light transmitted through the central portion of the retinal vessel in the absence of reflections from the central portion of the retinal vessel.

3. A method according to claim 2 wherein said step of determining the intensity of light transmitted through lateral portions of the retinal vessel comprises approximating a portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position, and wherein said creating step comprises fitting a curve to the portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position such that the fitted curve defines the intensity profile function.

4. A method according to claim 1 wherein said illuminating step comprises illuminating the retinal vessel and at least a portion of the background fundus with light defined by a finite point spread function, and wherein said constructing step further comprises compensating for the finite point spread function of the light such that the transmittance of blood within the blood vessel is more accurately determined.

5. A method according to claim 1 wherein said matching step comprises matching a portion of the detected light intensities with a W-shaped pattern.

6. A method according to claim 1 wherein said matching step comprises comparing the detected light intensities with a plurality of different patterns corresponding to the intensity of light that has propagated through respective ones of a plurality of different retinal vessels.

7. A method of measuring the transmittance of blood within a retinal vessel, the method comprising the steps of:

illuminating the retinal vessel and at least a portion of the background fundus within an eye;

detecting the intensity of light reflected from illuminated portions of the eye;

constructing an intensity profile function based upon the detected light intensity, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, and wherein said constructing step comprises compensating for at least some of the reflections of light from the retinal vessel which occurred prior to propagation of the light through the retinal vessel;

constructing a second intensity profile function which defines a curve and approximates the intensity of light reflected from the background fundus as a function of retinal position; and

determining the transmittance of blood within the retinal vessel based upon the intensity profile function, wherein said step of determining the transmittance of blood within the retinal vessel is based upon both the intensity profile function and the second intensity profile function.

8. A method according to claim 7 wherein said step of constructing a second intensity profile function comprises compensating for reflections of light at opposed edge portions of the retinal vessel.

9. A method according to claim 7 wherein said step of determining the transmittance of blood within the retinal vessel comprises:

dividing the intensity profile function by the second intensity profile function to form an intermediate transmittance function; and

determining a minimum value of the intermediate transmittance function, wherein the minimum value corresponds to the transmittance of the blood within the retinal vessel.

10. A method of measuring the transmittance of blood within a retinal vessel based upon intensity signals representing the intensity of light reflected from illuminated portions of an eye including the retinal vessel and a background fundus, the method comprising the steps of:

matching a portion of the intensity signals with a predetermined pattern corresponding to the intensity of light that has propagated through a retinal vessel so as to identify that portion of the intensity signals that has been transmitted through a retinal vessel;

constructing an intensity profile function based upon the identified portion of the intensity signals representing the intensity of light reflected from illuminated portions of the eye, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, and wherein said constructing step comprises compensating for at least some of the reflections of light from the retinal vessel which occurred prior to propagation of the light through the retinal vessel;

determining the intensity of light reflected from the background fundus based upon the intensity signals representing the intensity of light reflected from the background fundus; and

determining the transmittance of blood within the retinal vessel based upon the intensity profile function and the intensity of light reflected from the background fundus.

11. A method according to claim 10 wherein said constructing step further comprises determining the intensity of light transmitted through lateral portions of the retinal vessel based upon the intensity signals representing the intensity of light transmitted through lateral portions of the retinal vessel, wherein said compensating step comprises compensating for reflections of light from a central portion of the retinal vessel which occurred prior to propagation of the light through the retinal vessel, and wherein said compensating step further comprises creating the intensity profile function based upon the intensity of light transmitted through lateral portions of the retinal vessel such that the intensity profile function approximates the light transmitted through the central portion of the retinal vessel in the absence of reflections from the central portion of the retinal vessel.

12. A method according to claim 11 wherein said step of determining the intensity of light transmitted through lateral portions of the retinal vessel comprises approximating a portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position, and wherein said creating step comprises fitting a curve to the portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position such that the fitted curve defines the intensity profile function.

13. A method according to claim 10 wherein the retinal vessel and at least a portion of the background fundus are illuminated with light defined by a finite point spread function, and wherein said constructing step further comprises compensating for the finite point spread function of the light such that the transmittance of blood within the blood vessel is more accurately determined.

14. A method according to claim 10 wherein said matching step comprises matching a portion of the detected light intensities with a W-shaped pattern.

15. A method according to claim 10 wherein said matching step comprises comparing the detected light intensities with a plurality of different patterns corresponding to the intensity of light that has propagated through respective ones of a plurality of different retinal vessels.

16. A method of measuring the transmittance of blood within a retinal vessel based upon intensity signals representing the intensity of light reflected from illuminated portions of an eye including the retinal vessel and a background fundus, the method comprising the steps of:

constructing an intensity profile function based upon the intensity signals representing the intensity of light reflected from illuminated portions of the eye, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, and wherein said constructing step comprises compensating for at least some of the reflections of light from the retinal vessel which occurred prior to propagation of the light through the retinal vessel;

determining the intensity of light reflected from the background fundus based upon the intensity signals representing the intensity of light reflected from the background fundus, wherein said step of determining the intensity of light reflected from the background fundus comprises constructing a second intensity profile function which defines a curve and approximates the intensity of light reflected from the background fundus as a function of retinal position; and

determining the transmittance of blood within the retinal vessel based upon the intensity profile function and the second intensity profile function.

17. A method according to claim 16 wherein said step of constructing a second intensity profile function comprises constructing a linear function which approximates the intensity of light reflected from the background fundus as a function of retinal position.

18. A method according to claim 16 wherein said step of constructing a second intensity profile function comprises compensating for reflections of light at opposed edge portions of the retinal vessel.

19. A method according to claim 16 wherein said step of determining the transmittance of blood within the retinal vessel comprises the steps of:

dividing the intensity profile function by the second intensity profile function to form an intermediate transmittance function; and

determining a minimum value of the intermediate transmittance function, wherein the minimum value corresponds to the transmittance of the blood within the retinal vessel.

21. A method according to claim 18 wherein said fitting step comprises fitting a substantially U-shaped curve to the portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position.

20. A method of constructing an intensity profile function based upon intensity signals representing the intensity of light reflected from illuminated portions of an eye including a retinal vessel and a background fundus, wherein the intensity profile function approximates the intensity of light transmitted through a retinal vessel as a function of retinal vessel position, the method comprising the steps of:

determining the intensity of light transmitted through lateral portions of the retinal vessel based upon intensity signals representing the intensity of light reflected from illuminated portions of the eye, wherein said determining step comprises approximating a portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position; and

compensating for reflections of light from a central portion of the retinal vessel which occurred prior to propagation of the light through the retinal vessel, wherein said compensating step comprises creating the intensity profile function based upon the intensity of light transmitted through the lateral portions of the retinal vessel such that the intensity profile function approximates the light transmitted through the central portion of the retinal vessel in the absence of reflections from the central portion of the retinal vessel, wherein said creating step comprises fitting a cubic function to the portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position.

22. A method of analyzing blood within a retinal vessel based upon intensity signals representing the intensity of light reflected from illuminated portions of an eye including the retinal vessel and a background fundus, the method comprising the steps of:

constructing an intensity profile function based upon the intensity signals representing the intensity of light reflected from illuminated portions of the eye, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position;

providing a mathematical model of the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, wherein the mathematical model is a cubic function that includes a plurality of parameters including parameters describing the retinal vessel and parameters describing reflections of light from the retinal vessel which occurred prior to propagation of the light through the retinal vessel;

fitting the mathematical model to a portion of the intensity profile function that describes the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position; and

determining at least one parameter describing the retinal vessel following said fitting step.

23. A method according to claim 22 wherein the mathematical model includes a parameter defining the transmittance of blood within the retinal vessel, and wherein said determining step comprises approximating the transmittance of blood within the retinal vessel.

24. A method according to claim 22 wherein the mathematical model includes a parameter defining the diameter of the retinal vessel, and wherein said determining step comprises approximating the diameter of the retinal vessel.

25. An apparatus for measuring the transmittance of blood within a retinal vessel, the apparatus comprising:

an optical source for illuminating the retinal vessel and at least a portion of the background fundus within an eye;

a detector for measuring the intensity of light reflected from the illuminated portions of the eye;

means, responsive to said detector, for matching a portion of the measured light intensities with a predetermined pattern corresponding to the intensity of light that has propagated through a retinal vessel so as to identify that portion of the intensity signals that has been transmitted through a retinal vessel;

means, responsive to said detector, for constructing an intensity profile function based upon the matching portion of the measured light intensities, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, and wherein said constructing means comprises means for compensating for at least some of the reflections of light from the retinal vessel produced prior to propagation of the light through the retinal vessel; and

means, responsive to said constructing means, for determining the transmittance of blood within the retinal vessel based upon the intensity profile function.

26. An apparatus according to claim 25 wherein said constructing means further comprises means for determining the intensity of light transmitted through lateral portions of the retinal vessel, wherein said compensating means comprises means for compensating for reflections of light from a central portion of the retinal vessel which occurred prior to propagation of the light through the retinal vessel, and wherein said compensating means further comprises means for creating the intensity profile function based upon the intensity of light transmitted through the lateral portions of the retinal vessel such that the intensity profile function approximates the light transmitted through the central portion of the retinal vessel in the absence of reflections from the central portion of the retinal vessel.

27. An apparatus according to claim 26 wherein said means for determining the intensity of light transmitted through lateral portions of the retinal vessel comprises means for approximating a portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position, and wherein said creating means comprises means for fitting a curve to the portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position such that the fitted curve defines the intensity profile function.

28. An apparatus according to claim 25 wherein said optical source illuminates the retinal vessel and at least a portion of the background fundus with light defined by a finite point spread function, and wherein said constructing means further comprises means for compensating for the finite point spread function of the light such that the transmittance of blood within the blood vessel is more accurately determined.

29. An apparatus according to claim 25 wherein said means for matching comprises means for matching a portion of the detected light intensities with a W-shaped pattern.

30. An apparatus according to claim 25 wherein said means for matching comprises means for comparing the detected light intensities with a plurality of different patterns corresponding to the intensity of light that has propagated through respective ones of a plurality of different retinal vessels.

31. An apparatus for measuring the transmittance of blood within a retinal vessel, the apparatus comprising:

an optical source for illuminating the retinal vessel and at least a portion of the background fundus within an eye;

a detector for measuring the intensity of light reflected from the illuminated portions of the eye;

means, responsive to said detector, for constructing an intensity profile function based upon the measured light intensities, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, and wherein said constructing means comprises means for compensating for at least some of the reflections of light from the retinal vessel produced prior to propagation of the light through the retinal vessel;

means, responsive to said detector, for constructing a second intensity profile function which defines a curve and approximates the intensity of light reflected from the background fundus as a function of retinal position; and

means, responsive to said constructing means, for determining the transmittance of blood within the retinal vessel is based upon both the intensity profile function and the second intensity profile function.

32. An apparatus according to claim 31 wherein said means for constructing a second intensity profile function comprises means for compensating for reflections of light at opposed edge portions of the retinal vessel.

33. An apparatus according to claim 31 wherein said means for determining the transmittance of blood within the retinal vessel comprises:

means for dividing the intensity profile function by the second intensity profile function to form an intermediate transmittance function; and

means, responsive to said dividing means, for determining a minimum value of the intermediate transmittance function, wherein the minimum value corresponds to the transmittance of the blood within the retinal vessel.

34. A computer program product for determining the transmittance of blood within a retinal vessel based upon intensity signals representing the intensity of light reflected from illuminated portions of an eye including the retinal vessel and a background fundus, the computer program product comprising a computer-readable storage medium having computer-readable program code means embodied in said medium, said computer-readable program code means comprising:

first computer-readable program code means for constructing an intensity profile function based upon the intensity signals representing the intensity of light reflected from the illuminated portions of the eye, wherein the intensity profile function approximates the intensity of light transmitted through the retinal vessel as a function of retinal vessel position, wherein said first computer-readable program code means comprises computer-readable program code means for compensating for at least some of the reflections of light from the retinal vessel which occurred prior to propagation of the light through the retinal vessel;

second computer-readable program code means for determining the intensity of light reflected from the background fundus based upon the intensity signals representing the intensity of light reflected from the background fundus, wherein said second computer-readable program code means for determining the intensity of light reflected from the background fundus comprises computer-readable program code means for constructing a second intensity profile function which defines a curve and approximates the intensity of light reflected from the background fundus as a function of retinal position; and

third computer-readable program code means for determining the transmittance of blood within the retinal vessel based upon the intensity profile function constructed by said first computer-readable program code means and the second intensity profile function constructed by said second computer-readable program code means.

35. A computer program product according to claim 34 wherein said first computer-readable program code means further comprises computer-readable program code means for determining the intensity of light transmitted through lateral portions of the retinal vessel based upon the intensity signals representing the intensity of light transmitted through lateral portions of the retinal vessel, wherein said computer-readable program code means for compensating for at least some of the reflections from the retinal vessel comprises computer-readable program code means for compensating for reflections of light from a central portion of the retinal vessel which occurred prior to propagation of the light through the retinal vessel, and wherein said computer-readable program code means for compensating for reflections of light from a central portion of the retinal vessel comprises computer-readable program code means for creating the intensity profile function based upon the intensity of light transmitted through the lateral portions of the retinal vessel such that the intensity profile function approximates the light transmitted through the central portion of the retinal vessel in the absence of reflections from the central portion of the retinal vessel.

36. A computer program product according to claim 35 wherein said computer-readable program code means for determining the intensity of light transmitted through lateral portions of the retinal vessel comprises computer-readable program code means for approximating a portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position, and wherein computer-readable program code means for creating the intensity profile function comprises computer-readable program code means for fitting a curve to the portion of the intensity profile function describing the intensity of light transmitted through lateral portions of the retinal vessel as a function of retinal vessel position such that the fitted curve defines the intensity profile function.

37. A computer program product according to claim 34 wherein said computer-readable program code means for constructing a second intensity profile function comprises computer-readable program code means for compensating for reflections of light at opposed edge portions of the retinal vessel.

38. A computer program product according to claim 34 wherein said second computer-readable program code means for determining the transmittance of blood within the retinal vessel comprises:

computer-readable program code means for dividing the intensity profile function by the second intensity profile function to form an intermediate transmittance function; and

computer-readable program code means for determining a minimum value of the intermediate transmittance function, wherein the minimum value corresponds to the transmittance of the blood within the retinal vessel.

39. A computer program product according to claim 34 wherein the retinal vessel and at least a portion of the background fundus are illuminated with light defined by a finite point spread function, and wherein said first computer-readable program code means further comprises computer-readable program code means for compensating for the finite point spread function of the light such that the transmittance of blood within the blood vessel is more accurately determined.